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Access through your institution Buy or subscribe PARAFFIN-EMBEDDED TISSUE IS LESS ACCURATE THAN FROZEN SECTION ANALYSIS FOR DETERMINING VHL MUTATIONAL STATUS IN SPORADIC RENAL CELL CARCINOMA
Verhoest, G. _ et al_. _Urol. Oncol._ doi:10.1016/j.urolonc.2010.07.005 Determining whether a patient's renal tumor harbors mutated variants of the Von-Hippel Lindau (VHL) gene could
aid prognostication. A new study highlights the importance of the mode by which tissue is processed prior to extraction and sequencing of DNA. A team based in France analyzed clear-cell
renal cell carcinoma samples from 76 patients. Although DNA could be extracted from formalin-fixed, paraffin-embedded tissue, much of it was severely degraded. Subsequent amplification and
sequencing detected VHL mutations in just 52% of paraffinized samples, whereas analysis of frozen tissue yielded a 98% mutation rate. This is a preview of subscription content, access via
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* Learn about institutional subscriptions * Read our FAQs * Contact customer support RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE In brief. _Nat Rev
Urol_ 7, 593 (2010). https://doi.org/10.1038/nrurol.2010.181 Download citation * Published: 10 November 2010 * Issue Date: November 2010 * DOI: https://doi.org/10.1038/nrurol.2010.181 SHARE
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