1490 adaptation of renal sulfate (so4) reabsorption to sulfur intake

1490 adaptation of renal sulfate (so4) reabsorption to sulfur intake

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ABSTRACT Restriction of dietary sulfur results in reduced renal SO4 excretion, a finding attributed to decreased sulfur amino acid oxidation and filtered load of SO4. We found serum [SO4] in C57B1/6J adult male mice fed a “low-protein” (LP) diet (8% casein; 0.2% Met., 0.03% Cys) and in those fed a “high-protein” (HP) diet (27% casein; 0.68% Met., 0.1% Cys) for two weeks was not different (1.24±0.11 mM (x±SEM, n=6) vs 1.34±0.11 (n=7)). Reduced fractional SO4 excretion index (FEI SO4) was found in the LP group (LP=0.11±0.04 vs HP=0.50±0.14, p <.01). Evidence for adapted renal reabsorption was confirmed by assaying Na+-dependent uptake of 50 μM SO4 into renal brush border membrane vesicles (BBMV). Na+-dependent uptakes of 5 μM glucose and 50 μM phosphate served as internal markers; initial rates (15s) were normalized to those at equilibrium (60 min.). SO4 uptake was higher (361±39% of equilib. value n=10) in LP BBMV vs HP BBMV (270±16% n=10, p=0.023). No differences were noted with D-glucose (709±47% vs 698±30% n=5) or phosphate (193±17% vs 191±11%, n=11). The response to selective deficiency of dietary SO4 was studied using soybean-based diets with 1% NaCl (diet 1); 0.5% DL-methionine and 0.5% taurine (diet 2); and 1% Na2SO4 (diet 3). FEISO4 was 0.09± 0.01, 0.39±0.09, and 0.56±0.08, respectively. Adaptation was significant only with diet 1 (p <0.01). These findings indicate renal adaptation in BBM to depletion of SO4 _per se._ ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * MRC Genetics Group, McGill Univ.-Montreal Children's Hosp. Res. Inst., Montreal, Quebec, Canada David E C Cole & Charles R Scriver Authors * David E C Cole View author publications You can also search for this author inPubMed Google Scholar * Charles R Scriver View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Cole, D., Scriver, C. 1490 ADAPTATION OF RENAL SULFATE (SO4) REABSORPTION TO SULFUR INTAKE. _Pediatr Res_ 15 (Suppl 4), 691 (1981). https://doi.org/10.1203/00006450-198104001-01519 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01519 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT Restriction of dietary sulfur results in reduced renal SO4 excretion, a finding attributed to decreased sulfur amino acid oxidation and filtered load of SO4. We found serum [SO4] in


C57B1/6J adult male mice fed a “low-protein” (LP) diet (8% casein; 0.2% Met., 0.03% Cys) and in those fed a “high-protein” (HP) diet (27% casein; 0.68% Met., 0.1% Cys) for two weeks was not


different (1.24±0.11 mM (x±SEM, n=6) vs 1.34±0.11 (n=7)). Reduced fractional SO4 excretion index (FEI SO4) was found in the LP group (LP=0.11±0.04 vs HP=0.50±0.14, p <.01). Evidence for


adapted renal reabsorption was confirmed by assaying Na+-dependent uptake of 50 μM SO4 into renal brush border membrane vesicles (BBMV). Na+-dependent uptakes of 5 μM glucose and 50 μM


phosphate served as internal markers; initial rates (15s) were normalized to those at equilibrium (60 min.). SO4 uptake was higher (361±39% of equilib. value n=10) in LP BBMV vs HP BBMV


(270±16% n=10, p=0.023). No differences were noted with D-glucose (709±47% vs 698±30% n=5) or phosphate (193±17% vs 191±11%, n=11). The response to selective deficiency of dietary SO4 was


studied using soybean-based diets with 1% NaCl (diet 1); 0.5% DL-methionine and 0.5% taurine (diet 2); and 1% Na2SO4 (diet 3). FEISO4 was 0.09± 0.01, 0.39±0.09, and 0.56±0.08, respectively.


Adaptation was significant only with diet 1 (p <0.01). These findings indicate renal adaptation in BBM to depletion of SO4 _per se._ ARTICLE PDF AUTHOR INFORMATION AUTHORS AND


AFFILIATIONS * MRC Genetics Group, McGill Univ.-Montreal Children's Hosp. Res. Inst., Montreal, Quebec, Canada David E C Cole & Charles R Scriver Authors * David E C Cole View


author publications You can also search for this author inPubMed Google Scholar * Charles R Scriver View author publications You can also search for this author inPubMed Google Scholar


RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Cole, D., Scriver, C. 1490 ADAPTATION OF RENAL SULFATE (SO4) REABSORPTION TO SULFUR INTAKE. _Pediatr Res_


15 (Suppl 4), 691 (1981). https://doi.org/10.1203/00006450-198104001-01519 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01519 SHARE THIS


ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard


Provided by the Springer Nature SharedIt content-sharing initiative