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ABSTRACT Muscle histochemistry and ultrastructure was studied on percutaneous muscle biopsies from 3 patients with Reye's syndrome 3, 11 and 14 years old. Two or 3 sequential samples
were obtained from each patient using 4.0 and 5.0 mm Bergstrom needles. No complications were observed in spite of prolonged PT and PTT. H & E sections were normal but significant lipid
accumulation was seen on oil red 0 stained sections of tissue obtained between the first and tenth days of illness. The lipid was distributed fairly evenly throughout type I muscle fibers.
Lipid content decreased slowly and was normal after about 6 weeks. Oxidative enzyme and other histochemical stains (NADH, succinic dehydrogenase, ATPase, acid phosphatase, modified
trichrome) were normal. The major ultrastructural abnormality observed was loss of cristae in most mitochondria along with vacuolization and pleomorphic shapes. Lipid vacuoles were prominent
in the cytoplasm. One biopsy revealed numerous electron-dense crystalline arrays of 100-120 A particles, similar in appearance to virus particles. Ultrastructural changes returned to normal
after several weeks. Lipid accumulation and mitochondrial abnormalities in muscle tissue closely parallel those seen in liver tissue. Percutaneous muscle biopsy appears to be a safe and
effective technique for the diagnosis of Reye's syndrome. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departments of Pediatrics, Emanuel Hospital and University of Oregon
Health Sciences Center, Portland, Oregon John H Di Liberti & Robert C Neerhout Authors * John H Di Liberti View author publications You can also search for this author inPubMed Google
Scholar * Robert C Neerhout View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS
ARTICLE Di Liberti, J., Neerhout, R. 1567 MUSCLE ULTRASTRUCTURE AND HISTOCHEMISTRY IN REYE'S SYNDROME. _Pediatr Res_ 15 (Suppl 4), 704 (1981).
https://doi.org/10.1203/00006450-198104001-01584 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01584 SHARE THIS ARTICLE Anyone you share the
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