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ABSTRACT De novo fatty acid synthesis in fetal lung is probably an important source of the long chain fatty acid constituents of pulmonary surfactant and membrane phospholipids. In some
tissues, de novo fatty acid synthesis is regulated, in part, by the availability of exogenous fatty acids. Utilizing fetal rat lung explants, we measured de novo fatty acid synthesis by
determining the rate of 3H2O incorporation in saponifiable lipids. During a 48 hr. incubation in synthetic medium, 3H2O incorporation in explants from 19 day fetal rat lung increased from
15.1 nmoles/mg protein/hr to 34.2 nmoles/mg protein/hr. Incorporation into glycerides increased from 5.81 nmoles/mg protein/hr to 8.58 nmoles/mg protein/hr. 14C-glucose incorporation into
fatty acids also increased 2 fold during this time. Addition of 2.5mM palmitic acid to the incubation medium for 24 hrs. resulted in a 45% decrease in 3H2O and 14C-glucose incorporation into
fatty acids. There was,however,a 2.5 fold increase in 3H2O incorporation into glycerides. After 48 hrs. of culture in palmitate,3H2O incorporation into fatty acids was not different from
the control explants, while incorporation into glycerides by the explants cultured in palmitate continued to be 2.5 fold greater than controls. These data suggest that de novo fatty acid
synthesis in fetal lung explants is regulated in the short term by the availability of exogenous fatty acid. In addition, exogenous fatty acid results in an increase in the incorporation at
3H2O into the glyceride moiety. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, University of Rochester School of Medicine, Strong Memorial Hospital,
Rochester, NY. William M Maniscaico, Anita R Parkhurst & Donald L Shapiro Authors * William M Maniscaico View author publications You can also search for this author inPubMed Google
Scholar * Anita R Parkhurst View author publications You can also search for this author inPubMed Google Scholar * Donald L Shapiro View author publications You can also search for this
author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Maniscaico, W., Parkhurst, A. & Shapiro, D. 1690 EFFECTS OF EXOGENOUS
PALMITATE ON DE NOVO FATTY ACID SYNTHESIS IN FETAL LUNG EXPLANTS. _Pediatr Res_ 15 (Suppl 4), 725 (1981). https://doi.org/10.1203/00006450-198104001-01709 Download citation * Issue Date: 01
April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01709 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a
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