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ABSTRACT An assay for carbonic anhydrase activity was developed which measures pH deflection rate from the hydration of dissolved CO2. Enzymatic activity is expressed as ml CO2 hydration
catalysis per minute per mg Hgb or lung tissue protein at 22° C. 5 to 10 fetuses per litter at 25 through 31 days gestation were studied. Over this range amniotic fluid chloride fell from
114 ±2 to 110 ±1 meq/L and bicarbonate fell from 19 ±2 to 11 ±4 meq/L. The lung liquid chloride ranged from 144 ±10 to 158 ±2 , peaking at 27 days gestation. Bicarbonate ranged from 0.3 ±0.2
to 6.1 ±1, lowest at 27 days. Fetal RBC carbonic anhydrase activity correlated significantly with gestational age, rising from 14.9 ±3 to 34.9 ±4 ml CO2 hydration per minute/mg Hgb at 22°
C. Adult red cell activity was 84.6 ±13. Lung tissue carbonic anhydrase activity was independant of that in RBC, and ranged from 3.2 ±1.3 to 6.1 ±1.8 ml CO2/min per mg lung protein, and
directly correlated with fetal lung liquid Cl−/HCO3− ratios, peaking at 27 days gestation then decreasing to the levels found at term and in the adult rabbit lung. ARTICLE PDF AUTHOR
INFORMATION AUTHORS AND AFFILIATIONS * U. of Calif. San Diego, Dept. Pediatrics, La Jolla, Calif. J A Canfield & Louis Gluck Authors * J A Canfield View author publications You can also
search for this author inPubMed Google Scholar * Louis Gluck View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and
permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Canfield, J., Gluck, L. 229 CARBONIC ANHYORASE ACTIVITY IN ADULT AND FETAL RABBIT RED BLOOD CELLS AND LUNG TISSUE: A SIMPLE ASSAY AND
CORRELATION DURING DEVELOPMENT WITH LUNG LIQUID AND AMNIOTIC FLUID ANION COMPOSITION. _Pediatr Res_ 15 (Suppl 4), 478 (1981). https://doi.org/10.1203/00006450-198104001-00239 Download
citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-00239 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:
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