Rapid clearance of surfactant-associated palmitic acid from the lungs of developing and adult animals

Rapid clearance of surfactant-associated palmitic acid from the lungs of developing and adult animals

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ABSTRACT ABSTRACT: Palmitic acid is a minor component of natural surfactant and has been used to modify lipid extracts of natural surfactants to optimize their _in vitro_ surface properties. The metabolic fate of palmitic acid in surfactant is unknown. The clearance of surfactant-associated radiolabeled palmitic acid after intratracheal administration was investigated with trace doses of surfactant in the adult rabbit and with trace and treatment doses in the 28-d fetal rabbit and the 132-d fetal sheep. Palmitic acid was cleared rapidly from the airways, with less than 2% of the radiolabel recovered as free palmitic acid in the alveolar wash by l h in all models. Recovery as free palmitic acid in the total lung at 2 h was 2% in the adult rabbit and 3% both doses in the preterm rabbit. In the preterm sheep, the recovery as free palmitic acid in the total lung was approximately 2% of the trace dose and 1 % of the treatment dose by 5 h. Between 5 and 15% of the instilled palmitic acid was used as substrate for phospholipid synthesis by the lung in the different models. About 30% of the palmitate derived label was recovered in lipid extracts of liver 30 min after trachéal instillation of labeled surfactant in adult rabbits, whereas only 5—10% of the palmitate derived label was found in liver lipids in the preterm animals. In contrast to palmitic acid, radiolabeled triglycéride was cleared much more slowly from the airspaces and lungs of preterm sheep. Inasmuch as large amounts of palmitic acid are cleared rapidly from airspaces and lung tissue, it will not have a prolonged effect on the surface properties of surfactant but it may serve as a precursor for lung lipid metabolism. SIMILAR CONTENT BEING VIEWED BY OTHERS AEROSOL, CHEMICAL AND PHYSICAL PROPERTIES OF DRY POWDER SYNTHETIC LUNG SURFACTANT FOR NONINVASIVE TREATMENT OF NEONATAL RESPIRATORY DISTRESS SYNDROME Article Open access 12 August 2021 RESTORATION OF SURFACTANT ACTIVITY BY POLYMYXIN B IN LIPOPOLYSACCHARIDE-POTENTIATED INJURY OF IMMATURE RABBIT LUNGS Article Open access 08 January 2021 SURFACTANT LUNG DELIVERY WITH LISA AND INSURE IN ADULT RABBITS WITH RESPIRATORY DISTRESS Article 15 January 2021 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departments of Obstetrics and Pediatrics, Harbor-UCLA Medical School, UCLA School of Medicine, Terranee, 90509, California Bannie Tabor, Machiko Ikegami, Takako Yamada & Alan Jobe Authors * Bannie Tabor View author publications You can also search for this author inPubMed Google Scholar * Machiko Ikegami View author publications You can also search for this author inPubMed Google Scholar * Takako Yamada View author publications You can also search for this author inPubMed Google Scholar * Alan Jobe View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tabor, B., Ikegami, M., Yamada, T. _et al._ Rapid Clearance of Surfactant-Associated Palmitic Acid from the Lungs of Developing and Adult Animals. _Pediatr Res_ 27, 268–273 (1990). https://doi.org/10.1203/00006450-199003000-00013 Download citation * Received: 31 July 1989 * Accepted: 13 October 1989 * Issue Date: 01 March 1990 * DOI: https://doi.org/10.1203/00006450-199003000-00013 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT ABSTRACT: Palmitic acid is a minor component of natural surfactant and has been used to modify lipid extracts of natural surfactants to optimize their _in vitro_ surface properties.


The metabolic fate of palmitic acid in surfactant is unknown. The clearance of surfactant-associated radiolabeled palmitic acid after intratracheal administration was investigated with


trace doses of surfactant in the adult rabbit and with trace and treatment doses in the 28-d fetal rabbit and the 132-d fetal sheep. Palmitic acid was cleared rapidly from the airways, with


less than 2% of the radiolabel recovered as free palmitic acid in the alveolar wash by l h in all models. Recovery as free palmitic acid in the total lung at 2 h was 2% in the adult rabbit


and 3% both doses in the preterm rabbit. In the preterm sheep, the recovery as free palmitic acid in the total lung was approximately 2% of the trace dose and 1 % of the treatment dose by 5


h. Between 5 and 15% of the instilled palmitic acid was used as substrate for phospholipid synthesis by the lung in the different models. About 30% of the palmitate derived label was


recovered in lipid extracts of liver 30 min after trachéal instillation of labeled surfactant in adult rabbits, whereas only 5—10% of the palmitate derived label was found in liver lipids in


the preterm animals. In contrast to palmitic acid, radiolabeled triglycéride was cleared much more slowly from the airspaces and lungs of preterm sheep. Inasmuch as large amounts of


palmitic acid are cleared rapidly from airspaces and lung tissue, it will not have a prolonged effect on the surface properties of surfactant but it may serve as a precursor for lung lipid


metabolism. SIMILAR CONTENT BEING VIEWED BY OTHERS AEROSOL, CHEMICAL AND PHYSICAL PROPERTIES OF DRY POWDER SYNTHETIC LUNG SURFACTANT FOR NONINVASIVE TREATMENT OF NEONATAL RESPIRATORY


DISTRESS SYNDROME Article Open access 12 August 2021 RESTORATION OF SURFACTANT ACTIVITY BY POLYMYXIN B IN LIPOPOLYSACCHARIDE-POTENTIATED INJURY OF IMMATURE RABBIT LUNGS Article Open access


08 January 2021 SURFACTANT LUNG DELIVERY WITH LISA AND INSURE IN ADULT RABBITS WITH RESPIRATORY DISTRESS Article 15 January 2021 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *


Departments of Obstetrics and Pediatrics, Harbor-UCLA Medical School, UCLA School of Medicine, Terranee, 90509, California Bannie Tabor, Machiko Ikegami, Takako Yamada & Alan Jobe


Authors * Bannie Tabor View author publications You can also search for this author inPubMed Google Scholar * Machiko Ikegami View author publications You can also search for this author


inPubMed Google Scholar * Takako Yamada View author publications You can also search for this author inPubMed Google Scholar * Alan Jobe View author publications You can also search for this


author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tabor, B., Ikegami, M., Yamada, T. _et al._ Rapid Clearance of


Surfactant-Associated Palmitic Acid from the Lungs of Developing and Adult Animals. _Pediatr Res_ 27, 268–273 (1990). https://doi.org/10.1203/00006450-199003000-00013 Download citation *


Received: 31 July 1989 * Accepted: 13 October 1989 * Issue Date: 01 March 1990 * DOI: https://doi.org/10.1203/00006450-199003000-00013 SHARE THIS ARTICLE Anyone you share the following link


with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt


content-sharing initiative