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ABSTRACT ABSTRACT: Tissue responsiveness to growth hormone is likely to be regulated by local concentrations and availability of the membrane-bound growth hormone receptor (GHR) and perhaps
by the actions of the soluble growth hormone binding protein (GHBP). To determine whether the developmental regulation of the GHR and GHBP might vary among tissues, we have measured the
relative abundance of the 4.3-kb GFR and 1.3-kb GHBP mRNA in rat fetal and postnatal liver, kidney, lung, and ileum by Northern hybridization of polyadenylated RNA with a 32P-labeled
antisense riboprobe prepared from a rat GHR cDNA. The GHR and GHBP mRNA were both present in the four tissues studied at fetal age 19 d (E19). In postnatal liver, both transcripts increased
in abundance 3− to 4-fold after 14 d to mature levels at 42 d (_p_ = 0.0001). Similar changes were seen in postnatal kidney for GHR mRNA abundance; however, GHBP mRNA abundance increased
only 2− to 3-fold to mature levels by 28 d (kidney GHR _versus_ GHBP mRNA profile, _p_ = 0.0001). In lung, a 2-fold linear increase in GHR mRNA abundance was observed (_p_ = 0.0019), but the
GHBP mRNA did not change (GHR _versus_ GHBP mRNA profile, _p_ = 0.0006). Both transcripts decreased in abundance by 2− to 3-fold from E19 to 42 d in ileum (_p_ < 0.05). The abundance of
both transcripts was three to 10 times greater in 60-d liver than in the other three tissues at 60 d. The variation in abundance and in the developmental profiles of the GHR and GHBP mRNA
observed in these fetal and postnatal tissues suggests that the GHR and GHBP could mediate differences within and between tissues in the responsiveness to growth hormone. The differential
regulation of the two transcripts evident in kidney and lung supports the emerging evidence that the GHBP may have a funciton distinct from that of the GHR. SIMILAR CONTENT BEING VIEWED BY
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ETHANOLAMINE PLASMALOGENS IN OLD BOVINE BRAINS Article Open access 26 February 2021 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Pediatric Endocrinology, University
of North Carolina at Chapel Hill, 27599–7220, North Carolina Jan L Walker, Billie M Moats-Staats & Louis E Underwood * Division of Neonatology, University of North Carolina at Chapel
Hill, 27599–7220, North Carolina Alan D Stiles Authors * Jan L Walker View author publications You can also search for this author inPubMed Google Scholar * Billie M Moats-Staats View author
publications You can also search for this author inPubMed Google Scholar * Alan D Stiles View author publications You can also search for this author inPubMed Google Scholar * Louis E
Underwood View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Walker,
J., Moats-Staats, B., Stiles, A. _et al._ Tissue-Specific Developmental Regulation of the Messenger Ribonucleic Acids Encoding the Growth Hormone Receptor and the Growth Hormone Binding
Protein in Rat Fetal and Postnatal Tissues. _Pediatr Res_ 31, 335–339 (1992). https://doi.org/10.1203/00006450-199204000-00006 Download citation * Received: 17 September 1991 * Accepted: 04
December 1991 * Issue Date: 01 April 1992 * DOI: https://doi.org/10.1203/00006450-199204000-00006 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this
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