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Abstract 59 Introduction: Lipid A is responsible for the endotoxic activities of lipopolysaccharides in gram-negative infection. Since there are no tests to prove direct endotoxic intraction
with membranes, we analyzed lipid A by measuring the content of hydroxymyristic acid (HMA). The HMA is not found in human tissues but only in bacterial endotoxins. Materials: Washed red
blood cells (RBC) from 5 neonates and 5 adults were incubated with 100 µg of lipid A for 30 and 60 minutes. RBC membranes were isolated and the hydoxymyristic acid content was qualitatively
and quantitatively analysed by gas chromatography. Results: Lipid A was detectable in all RBC membranes. After 30 min of incubation the HMA content was 459.7 ± 16.8 pmol/ml RBC in neonates
and 423.3 ± 12.7 pmol/ml RBC in adults. After 60 min of incubation 518.9 ± 99.9 in neonates and 491.5 ± 99.2 in adults(Means ± SEM). Conclusion: This study shows, that lipid A interacts
directly with RBC membranes. The effect is time dependent. Neonatal RBC are slightly more susceptible for lipid A insertion into their membranes than adult RBC. Approximately 1% of the lipid
A was detected in the RBC membranes after incubation indicating that this method is effective to detect minimal amounts of endotoxin in membranes. Further studies should be done to detect
lipid A in endothelial cells during infection. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Neonatology, Dept. of Pediatrics, University of Heidelberg, Germany JMB Pöschl &
O Linderkamp * Etablissement de Transfuion Sanguine, Strasbourg, France C Leray & J P Cazenave Authors * JMB Pöschl View author publications You can also search for this author inPubMed
Google Scholar * C Leray View author publications You can also search for this author inPubMed Google Scholar * J P Cazenave View author publications You can also search for this author
inPubMed Google Scholar * O Linderkamp View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE
CITE THIS ARTICLE Pöschl, J., Leray, C., Cazenave, J. _et al._ Lipid A from gram-negative bacteria is detectable in neonatal and adult erythrocyte membranes. _Pediatr Res_ 44, 429 (1998).
https://doi.org/10.1203/00006450-199809000-00092 Download citation * Issue Date: 01 September 1998 * DOI: https://doi.org/10.1203/00006450-199809000-00092 SHARE THIS ARTICLE Anyone you share
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