ABSTRACT MicroRNAs have been involved in insulin resistance (IR). As the mechanism whereby niacin, an anti-dyslipidemic agent, leads to IR remains elusive, we sought to identify

differentially expressed microRNAs in adipose tissue (AT) of individuals receiving niacin and to explore the link between microRNAs, niacin and IR in human adipocytes. In a double-blind

controlled study, 22 obese men received extended-release niacin or placebo over 8 weeks. Bioclinical data and subcutaneous AT biopsies were obtained before and after treatment. AT microRNA

expression profiles were determined using RTqPCR for 758 human-specific microRNAs. hMADS adipocytes were treated with niacin, or acipimox (a niacin-like drug without effect on IR), or

adipocytes led to reduced insulin-induced glucose uptake and lower insulin-stimulated AKT phosphorylation. Long term niacin treatment altered microRNA expression levels in human AT.

Increased miR-502-3p expression may play a role in the mediation of IR due to niacin in adipocytes. The study is registered in Clinical Trials NCT01083329 and EudraCT 2009-012124-85. Access

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technical support. DL is a member of Institut Universitaire de France. FUNDING The work was supported by grants from the National Research Agency ANR-09-GENO-0018-01 (to D. Langin), Inserm

DHOS Recherche Translationnelle 2009 (to D. Langin), AOL-0816302 Hôpitaux de Toulouse (to D. Langin), Glaxo Smith Kline (to D. Langin), and JPI HDHL-miRDiet (to N.Viguerie and D. Langin).

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut National de la Santé et de la Recherche Médicale (Inserm), UMR1048, Obesity Research Laboratory, Institute of Metabolic and

Cardiovascular Diseases (I2MC), Toulouse, France Emilie Montastier, Diane Beuzelin, Frédéric Martins, Lucile Mir, Marie-Adeline Marqués, Jason Iacovoni, Dominique Langin & Nathalie

Viguerie * University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France Emilie Montastier, Diane Beuzelin, Dominique Langin & Nathalie Viguerie * Departments of

Endocrinology, Toulouse University Hospitals, Metabolism and Nutrition, and Clinical Biochemistry, Toulouse, France Emilie Montastier & Dominique Langin * Institut National de la Santé

et de la Recherche Médicale (Inserm), UMR1048, Plateforme GeT (Génome et Transcriptome) du Génopole, Toulouse, France Frédéric Martins * Institut National de la Santé et de la Recherche

Médicale (Inserm), Clinical Investigation Center 1436, Toulouse, France Claire Thalamas * Toulouse University Hospitals, Clinical Investigation Center 1436, Toulouse, France Claire Thalamas

Authors * Emilie Montastier View author publications You can also search for this author inPubMed Google Scholar * Diane Beuzelin View author publications You can also search for this author

inPubMed Google Scholar * Frédéric Martins View author publications You can also search for this author inPubMed Google Scholar * Lucile Mir View author publications You can also search for

this author inPubMed Google Scholar * Marie-Adeline Marqués View author publications You can also search for this author inPubMed Google Scholar * Claire Thalamas View author publications

You can also search for this author inPubMed Google Scholar * Jason Iacovoni View author publications You can also search for this author inPubMed Google Scholar * Dominique Langin View

author publications You can also search for this author inPubMed Google Scholar * Nathalie Viguerie View author publications You can also search for this author inPubMed Google Scholar

CORRESPONDING AUTHOR Correspondence to Nathalie Viguerie. ETHICS DECLARATIONS CONFLICT OF INTEREST The authors declare that they have no conflict of interest. ELECTRONIC SUPPLEMENTARY

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ABOUT THIS ARTICLE CITE THIS ARTICLE Montastier, E., Beuzelin, D., Martins, F. _et al._ Niacin induces miR-502-3p expression which impairs insulin sensitivity in human adipocytes. _Int J

Obes_ 43, 1485–1490 (2019). https://doi.org/10.1038/s41366-018-0260-5 Download citation * Received: 09 April 2018 * Revised: 14 September 2018 * Accepted: 27 September 2018 * Published: 27

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