ABSTRACT Intentional tooth replantation (ITR) is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions. ITR

is defined as the deliberate extraction of a tooth; evaluation of the root surface, endodontic manipulation, and repair; and placement of the tooth back into its original socket. Case

reports, case series, cohort studies, and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage

with root canal treatment or endodontic microsurgery. However, variations in clinical protocols for ITR exist due to the empirical nature of the original protocols and rapid advancements in

periodontitis or anatomical aberration, and the main complications of this treatment, aiming to refine the clinical management of ITR in accordance with the progress of basic research and

clinical studies; the findings suggest that ITR may become a more consistent evidence-based option in dental treatment. SIMILAR CONTENT BEING VIEWED BY OTHERS EXPERT CONSENSUS ON

REGENERATIVE ENDODONTIC PROCEDURES Article Open access 01 December 2022 MANAGING FAILED VITAL PULP THERAPIES IN MATURE PERMANENT TEETH IN A RETROSPECTIVE COHORT STUDY, WITH SUCCESS AND

SURVIVAL RATES OF MANAGING PROTOCOLS Article Open access 21 May 2024 ENDODONTIC AND DENTAL IMPLANT TREATMENT: KEY CONSIDERATIONS AND COMPARISONS Article Open access 23 May 2025 INTRODUCTION

Pupal and periradicular pathosis, caused by caries, trauma, or dental abnormalities, is a common disease requiring dental treatment in the clinic. Root canal treatment (RCT) is the

conventional option for managing endodontic diseases, with a pooled success rate of 92.6%.1,2 For teeth with persistent pain or periapical lesions after RCT, nonsurgical retreatment and

endodontic microsurgery (EMS) should always be considered. However, in specific cases with inaccessible endodontic or resorptive lesions or lesions adjacent to vital structures, endodontic

retreatment and EMS are impractical. In these situations, intentional tooth replantation (ITR) may be the last resort for preserving natural teeth.3 ITR is one of the oldest known methods

for the treatment of endodontic diseases, dating as far back as the 10th century, when the Chinese doctor Huaiyin Wang described it in his medical classic “_General Records of Holy Universal

Relief_”. Specifically, he stated that avulsed teeth should be replanted into the alveolar socket and fixed with wrought copper for approximately five days for stabilization. Subsequently,

Abulcasis, a famous Arab surgeon, performed a classic ITR procedure in the 11th century, and since then, multiple accounts of ITR have been reported. ITR was formally defined as “the

purposeful removal of a tooth and its almost immediate replacement, with the objective of obturating the canals apically when the tooth is out of the alveolar socket” by Grossman in 1982.4

The evolution of this procedure has involved the modification of techniques for tooth extraction, root-end resection and preparation; the handling of the tooth during surgical manipulation;

and the materials used for root-end filling. Notably, with the development of stem cell therapy, microtechnology and osteo-inductive biomaterials, the overall success rate of ITR has

significantly increased to 89.1% according to rigorous evidence-based analysis.5 Choi reported that the success rates of ITR at 4 years post-operation were 91.2%,6 while Pisano

systematically evaluated the overall success rate of ITR in single and multiple teeth as 86.7%.7 The efficacy of ITR in the retention of refractory and untreatable teeth that cannot be

managed with conventional methods has gradually been accepted by more clinicians.8,9 To date, more than 300 articles have been published, as found in PubMed through a search with the

keywords “ITR”, “tooth replantation”, “tooth transplantation” and “autotransplantation of tooth”. The article types included basic studies, case reports, case series, retrospective studies,

prospective clinical trials, reviews and guidelines; the most common article types were case reports and reviews. At present, two guidelines on ITR are available for dental practitioners.

The American Association of Endodontists (AAE) published clinical guidelines for ITR in 2019, briefly outlining the indications, clinical procedures, and treatment goals of ITR. The European

Society of Endodontology (ESE) position statement was published in 2021 and provides additional procedure details, including considerations in case selection, clinical protocols, and

follow-up requirements.10 Chinese scholars have conducted extensive exploration of ITR in both preclinical and clinical studies, gradually guiding primary care physicians to implement this

technique.11,12,13,14,15 This expert consensus systematically described the biological foundation, case selection, clinical protocol and outcome management of ITR, aiming to standardize the

clinical practice of this technique and extend tooth longevity. BIOLOGICAL FOUNDATION OF ITR ETIOLOGY Bacteria within the root canal are the etiological basis for periapical tissue lesions.

When the affected tooth exhibits abnormal dental structures or complex root canal systems, conventional endodontic treatment often fails to eradicate infection inside and outside the root

canal, leading to treatment failure.16 The advantages of ITR include the ability to thoroughly inspect and treat the root surface and periradicular area under direct vision and the ability

to address areas that are difficult to manage with conventional root canal treatment or EMS, thus eliminating various etiological factors, controlling infection, and preserving the affected

tooth.17 Advancements in modern EMS have provided substantial technical possibilities for enhancing the efficacy of ITR.18 TISSUE PRESERVATION AND REGENERATION The proper preservation and

regeneration of periodontal tissues are critical for healing after ITR.19 The periodontal ligament is a dense connective tissue located between the tooth root and alveolar bone, with one end

embedded in the cementum and the other extending into the alveolar bone, anchoring the tooth root and alleviating chewing pressure. The periodontal ligament is mainly composed of collagen

fibers, matrix, periodontal ligament cells, cementoblasts, and neurovascular components. When a tooth is extracted, its periodontal ligament is usually bisected, with the inner layer

attaching to the root surface and the rest remaining within the alveolar socket. Healing of the traumatized periodontal ligament involves two processes: revascularization of injured tissues

and formation of new tissue. Initially, after extraction, macrophages, endothelial cells, fibroblasts, and other cells migrate in a coordinated manner to the injured area, forming vascular

buds predominantly composed of immature type III collagen and proliferating fibroblasts in the matrix and repairing periodontal tissues at a rate of approximately 0.5 mm/day. After one week,

the fractured collagen fibers reconnect, stabilizing the replanted tooth. By the second week, most of the principal fibers are repaired, and the periodontal ligament regains mechanical

strength equivalent to approximately 2/3 of that of the original ligament. Cell proliferation, migration, and differentiation are the foundations of tissue regeneration. Periodontal ligament

cells constitute a heterogeneous population of cells, including osteoblasts, odontoblasts, cementoblasts, fibroblasts, and various precursor cells. Precursor cells possess multidirectional

differentiation potential and, upon differentiating into osteoblasts, cementoblasts, and fibroblasts, participate in maintaining the stability of the periodontal microenvironment for soft

and hard tissues. After tooth extraction, residual periodontal tissue remains at both the alveolar socket and the root surface, with more periodontal ligament stem cells (PDLSCs) on the

alveolar socket side. Studies have shown that PDLSCs derived from the alveolar socket possess stronger differentiation and bone regeneration capabilities than those derived from the root

surface, highlighting the importance of preserving residual periodontal ligament tissue within the alveolar socket in establishing new periodontal attachments during ITR.20 BIOMECHANISM OF

MATERIALS The materials used for root-end filling and perforation repair during ITR directly contact periodontal tissues. The sealing ability, biocompatibility, and potential biological

regulatory and antibacterial properties of these materials also influence the process of tissue repair and regeneration.21 Calcium silicate cements (CSCs), with formulations such as mineral

trioxide aggregate (MTA), iRoot BP Plus, and Biodentine, are currently the most commonly used materials for root-end filling and perforation repair. CSCs exhibit excellent sealing properties

and morphological stability and can release calcium, silicon and other inorganic ions in the fluid environment, affecting the biological behavior of surrounding cells.22,23 Research has

shown that MTA can enhance the osteogenic differentiation potential of PDLSCs by activating the NF-κB and MAPK signaling pathways,24 while iRoot BP Plus can upregulate the autophagy level of

bone marrow mesenchymal stem cells (BMSCs) and promote their osteogenic differentiation capacity.25 EMSs were performed in beagle models of apical periodontitis with MTA and iRoot BP Plus

as root-end filling materials, and postoperative cone-beam computed tomography (CBCT) revealed that both materials achieved good apical sealing. However, iRoot BP Plus promoted better

healing of the periapical bone and the formation of cementum-like, periodontal ligament-like, and bone tissue on the apical resection surface.26 Furthermore, during the healing process, CSCs

release hydroxyl ions that permeate into the dentin, increasing the pH value of the surrounding environment and thereby exerting certain antibacterial effects against common pathogenic

bacteria in infected root canals, such as _Enterococcus faecalis_ and _Candida albicans_.27,28 It has been suggested that MTA exhibits certain antibacterial properties before complete

healing, but after healing, the release of hydroxyl ions decreases, leading to the loss of its antibacterial properties.29 Notably, the antibacterial properties of other CSCs, such as iRoot

BP and Biodentine, do not differ significantly from those of MTA.30,31 Currently, the assessment of the antibacterial properties of CSCs is limited to in vitro studies, with few reports on

changes in pathogenic bacterial populations following in vivo application, thereby restricting their clinical significance. CASE SELECTION INDICATIONS Teeth with pulpal and periapical

diseases that remain unresponsive to nonsurgical RCT. * Cases where the canal cannot be accessed for retreatment, such as teeth restored with crowns, post-and-core crowns, or bridges. *

Cases where the efficacy of nonsurgical treatment is uncertain, such as blocked canals and untreatable root canal perforations.30,31 Teeth unsuitable for EMS. * Cases where establishing a

surgical pathway for EMS is difficult, due to: those with restricted mouth opening, thick buccal fat pad, shallow vestibular sulcus, prominent external oblique ridge of the mandible, lingual

inclination of the root apex, or posterior tooth positioning.32,33 * Cases where teeth are adjacent to vital anatomical structures, such as the maxillary sinus, inferior alveolar nerve

canal, mental foramen, or lingual artery branches, in which EMS poses a risk of damaging, leading to postoperative complications such as paralysis or bleeding.34,35 * Cases where teeth have

unrepairable root perforations, external root resorption, or cervical resorptive lesions, where apical surgery may cause excessive damage to alveolar bone and roots.36,37 Teeth with severe

developmental abnormalities of anatomical structures, such as invaginated lingual grooves.38,39 _Patients strongly requesting retention of a single-rooted tooth with a vertical root fracture

that does not extend through the entire root transection. In these cases, the tooth may be extracted and replanted after resin repair of the fracture_.40,41 CONTRAINDICATIONS *

Uncontrollable periodontal disease: Traditional guidelines consider periodontitis a contraindication for ITR42; however, current views suggest that root planning can be performed under

direct vision during ITR to achieve better access and debridement than during conventional periodontal flap surgery. Some scholars believe that severe periodontitis is not an absolute

contraindication for ITR.43 Comprehensive preoperative analysis of periodontal support is recommended to assess therapeutic efficacy.44 * Complete vertical root fracture. * Inadequate

supporting bone due to buccal or lingual alveolar bone defects, or insufficient alveolar bone in the furcation area, etc. * Extensive loss of tooth structure in the affected tooth that

cannot be restored functionally through restorative treatment. * Difficulties in complete extraction due to a large root bifurcation angle or root deformities. * Elderly patients with

systemic complications and compromised tissue healing and regenerative capabilities.13 PREOPERATIVE ASSESSMENT GENERAL HEALTH ASSESSMENT Patients should be evaluated for cardiovascular

disease, diabetes, and immune diseases; long-term use of oral or intravenous bisphosphonates; long-term oral or intravenous injection of high-dose corticosteroids; and the presence of

artificial implants such as cardiac valves and artificial joints. ORAL ASSESSMENT The condition of the prosthesis, root, periapical area, periodontal condition and adjacent structures should

be assessed. CBCT examinations are recommended for assessing the number, distribution, and curvature of roots, especially in cases of root fractures. Preoperative RCT is generally

recommended. TREATMENT PLAN For patients who have unsatisfactory results with nonsurgical retreatment or apical surgery, ITR is not the only option; extraction followed by implant

restoration is also a viable option (Table 1 and Fig. 1). CLINICAL PROTOCOL PATIENT INFORMED CONSENT Prior to any treatment, patients should be provided with complete information on their

medical condition, treatment alternatives, cost, and prognosis. The routine informed consent includes a comprehensive overview of the procedures, risks and benefits of the treatment, as well

as alternative treatment modalities. Once patients have been fully informed, the informed consent form should be signed before initiating treatment. Generally, the affected teeth are

pulpless which are brittle and susceptible to fracture during the process of extraction and inevitably cannot be retained. Thus, the operator must ensure the patient’s complete understanding

of possible complications, including tooth fracture and loosening of crown restoration, based on complete communication prior to surgery. MANAGEMENT OF SURGICAL AREA PREOPERATIVE

PERIODONTAL MAINTENANCE In preparation for surgery, comprehensive supragingival scaling and root planning are performed approximately one week prior to the scheduled procedure. Additionally,

the administration of 0.12% chlorhexidine is recommended to minimize the risk of bacterial infection. A thorough evaluation of the gingival and periodontal condition of the affected teeth

is conducted before surgical intervention. ANTIBIOTIC PROPHYLAXIS Antibiotic prophylaxis is not routinely recommended but is mandatory for medically compromised patients. The ESE guidelines

recommend the use of prophylactic antibiotics for patients with a history of congenital heart disease, prosthetic heart valves, or infective endocarditis, as well as those who have received

intravenous bisphosphonate therapy or undergone joint surgery within the past three months.10 DISINFECTION OF THE SURGICAL AREA The ESE guidelines recommend the use of an oral mucosa

disinfectant, usually 0.12% or 0.2% chlorhexidine, for 1 minute.10 EXTRACTION OF THE AFFECTED TOOTH Tooth extraction is the most technically challenging aspect of the ITR procedure (Fig. 2).

The tooth should be extracted intact with minimal damage to the surrounding periodontium. This critical step is essential for subsequent periodontium regeneration and successful

implantation. INSTRUMENTS AND TECHNIQUES Apical elevators, which can result in significant damage to the alveolar bone, periodontal ligament, and root surface cementum, are not recommended

due to the greater risk of external root resorption and other postoperative complications. Instead, tooth extraction tools that cause less trauma to the periodontium, such as dental forceps,

should be used.45 Preoperative CBCT is helpful for accurately assessing the shape and orientation of the roots. The appropriate type of extracting forceps should be selected based on tooth

position and morphology. Placing the beak of the forceps coronal to the cementoenamel junction without contact with the cementum and alveolar fossa can prevent damage to the periodontal

ligament. Approximately 2 mm of attached tissue above the alveolar crest is left, which allows the tooth forceps to slide downward.46 The tooth forceps should be used with careful and gentle

pressure.47 A rubber band on the handle can aid in maintaining a constant pressure during the extraction process. It is not necessary to rush the extraction, and the alveolar socket can be

slowly enlarged to the buccal-lingual side with a minor rotation before performing the dislocation. In cases where there are significant overfilling or separating instruments extend beyond

the apical foramen, periapical radiographs should be taken after extraction to confirm that no gutta-percha or separating instruments remain in the alveolar fossa. ADVANCED MINIMALLY

INVASIVE INSTRUMENTS AND TECHNIQUES When a tooth exhibits cervical malabsorption and subgingival decay, traditional forceps may cause root fractures. Krug and colleagues investigated a

minimally invasive tooth extraction system for anterior teeth and premolars, which involved placing a retainer drill in the root canal of the affected tooth, installing a retainer device on

the adjacent tooth, and using a wheel shaft force to pull and dislocate the affected tooth. These procedures can avoid applying pressure on the periodontium of the affected tooth, and the

incidence of postoperative complications is only 9.7%.48,49 However, the use of the minimally invasive tooth extraction system is currently limited to anterior teeth and premolars. Further

research is needed to evaluate its effectiveness for molars. Studies have demonstrated that prior disruption of the periodontal ligament connection can significantly reduce the difficulty of

tooth extraction and improve the success rate. For instance, orthodontic extrusion can be performed 2–3 weeks before extraction for teeth with multiple roots and thick buccal cortical

bone.6 During tooth extraction, Jang et al. first inserted a 15-gauge blade parallel to the periodontal ligament space along the direction of the root. By tapping the handle, the blade was

wedged in the direction of the root tip to cut off the periodontal ligament and reduce resistance during tooth extraction. Additionally, it is suggested that an O-shaped rubber band be

placed between the affected tooth and adjacent tooth 3–4 days before surgery to facilitate the release of the anchored connection between the affected tooth and the alveolar socket.50

However, retrospective analyses of the relationship between preoperative destruction of the periodontal ligament connection and postoperative healing of the periodontium are lacking.

Meticulous planning is required before deciding on the optimal extraction method, including a comprehensive evaluation of the difficulty of tooth extraction and the periodontal status.

MANAGEMENT OF THE ALVEOLAR SOCKET The key to effective alveolar socket management is maintaining the integrity of the periodontal ligament while properly removing diseased tissue. The extent

and techniques of curettage in the alveolar socket depend on specific cases. Generally, apical granulation can be removed during tooth extraction, and residual minor granulomas can be

tolerated especially when the lesion has been approximated to or invaded anatomic structures. Socket curettage can be performed under the magnification of a dental operating microscope with

appropriate excavators or tissue forceps when large granulomas or cysts need to be removed. Scratching the alveolar socket with a curette is not recommended. During extraoral procedures, the

protection of the alveolar socket with wet sterile gauze can help to prevent contamination with saliva or food residue.10 MANAGEMENT OF THE EXTRACTED TOOTH After complete extraction,

removal of the granulation tissue on the tooth with tissue forceps is recommended. To minimize damage to critical periodontal cells on the root surface, it is advisable to refrain from

direct scraping of the root surface. The root surface should be stained with methylene blue and carefully examined under high magnification with an operating microscope for the presence of

anatomical structures such as fractures, perforations, and additional root canals or isthmuses. During the examination, dental tweezers or forceps should be held on the coronal side of the

tooth to avoid damaging the periodontal ligament and cementum on the root surface. The effect of extraoral management of teeth is dependent on the operation time and preservation medium,

which are critical factors for determining the prognosis of replantation.51 The total extraoral operation time should be minimized and ideally controlled within 10-15 minutes to reduce the

risk of periodontal ligament cell necrosis and root resorption after

replantation.10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55 Pohl et al. identified extraoral

operation time as a predictor of major complications, with the risk of postoperative complications increasing by approximately 1.7 times when the procedure time exceeded 15 minutes.56,57 The

use of a liquid medium that preserves the activity of the periodontal ligament during the entire extraoral process is recommended. Hank’s balanced salt solution (HBSS) is the preferred

preservative, with a pH close to 7 and osmolality of 270-290 mOsm/L, which provides suitable conditions for the survival of periodontal ligament cells.58,59 However, HBSS is relatively

expensive and not widely available in some countries. Sterile normal saline (0.9% sodium chloride solution) and other electrolyte solutions, such as milk, are commonly used as preservation

media, although they lack the trace elements and nutrients necessary for cell survival and can only be used as short-term storage media (approximately 2 hours).60 Systematic reviews have

shown that HBSS, electrolyte solution, and milk can maintain more than 80% of periodontal ligament activity when used for up to 2 hours.61 During extraoral operations, the high-speed

handpiece water outlet should be turned off to prevent contamination of the tooth. The crown of the tooth should be held with wet sterile gauze and dental forceps while continuously rinsing

with the preservation medium to keep the root surface wet throughout the entire extraoral procedure. CSC root-end filling materials can be set and hardened in a humid environment,

eliminating the need to dry the tooth during the root-end filling process. MANAGEMENT OF THE ROOT APEX APICOECTOMY This procedure should be carried out with a magnification system. To resect

the root end effectively, a Lindemann bur should be applied with a 45° angled handpiece, and piezosurgery is also suggested.62 Previous studies have shown that when the operating frequency

of ultrasonic bone curettage coincides with the natural frequency of hard tissue, hard tissue is selectively resected without damaging soft tissue. The cavitation effect produced by

ultrasonic bone curettage can reduce bleeding in the operation area while resulting in a smooth root-cutting surface that is conducive to the formation of new periodontal attachments. Root

resection 3 mm from the apex is generally recommended to reduce most of the apical ramifications and lateral canals. However, some cases require more than 3 mm of root-end resection, such as

an apex in proximity to adjacent anatomical structures such as the mandibular canal and maxillary sinus, the presence of multiple accessory canals at the level of root resection, the

presence and location of vertical microcracks, or excess lingual inclination of the root.63 Other factors, such as the level of crestal bone, the shape of the root, the thickness of the

dentinal walls, and the apical extent of a post, may dictate the level of apical resection. The occurrence of apical root resorption may require less root resection. The apical extent of a

post no more than 5 mm from the root apex may necessitate less coronal resection based on an evaluation of the accessory canals to ensure the depth of root-end preparation and root-end

filling. Furthermore, the root section should be perpendicular to the long axis of the tooth, with a bevel angle of less than 10°, to ensure optimal removal of anatomic variations, such as

apical ramifications, accessory canals, and severe curves that are difficult to access during RCT. It is also important to completely remove the apical pathologic processes, reduce the

exposure of dentinal tubules at the resected root surface, and preserve more buccal supporting bone. ROOT-END PREPARATION During root-end preparation, an ultrasonic tip selected according to

the tooth position is aligned parallel to the long axis of the root and along the root prominence to make a box-like cavity at least 3 mm deep with a specific retention form.64 During

preparation, it is crucial to maintain gentle and intermittent movements while avoiding excessive pressure on the lateral walls to prevent microcracks. Additionally, it is important to

maintain a dental wall thickness of approximately 2 mm. Notably, root canal depressions, isthmuses, or variant anatomies also require preparation to ensure complete sealing of the root canal

system and periapical tissue. Spraying with a preservation medium such as HBSS and sterile saline is recommended during preparation to prevent tissue overheating. After preparation, any

remaining gutta-percha should be compacted with a microcondenser. ROOT-END FILLING The root-end filling materials should be at least 3–4 mm thick. After the root-end filling material is

compacted with a microplugger, excess material is removed with a wet cotton pellet to clean the resected root surface. The sealing properties, structural stability, and biocompatibility of

root-end filling materials are crucial for the prognosis of ITR.65 MTA is regarded as the gold standard for root-end filling materials due to its excellent sealing ability and

biocompatibility.66 However, some studies have shown that the success rate of MTA is limited in ITR teeth with C-shaped canals, which may be attributed to the difficulty in achieving

hemostasis of the replanting area, the presence of anatomic variations such as the isthmus in the C-shaped canals, and the lengthy setting time of MTA.50 Blood fluids in the apical sealing

area may affect MTA setting, mechanical properties, or sealing of microstructures such as the isthmus, which may lead to secondary infection. As a result, white MTA with a shorter setting

time is recommended over gray MTA to avoid being washed out by the gingival sulcus. Furthermore, restricted mobility of the replanted tooth can reduce compression to the sealing material

during occlusion to maintain a stable sealing effect.63,64,65,66,67 The development of new CSCs, such as iRoot BP Plus, has partially addressed the limitations of MTA, such as its long

healing time and ability to handle difficulties, and it is currently widely

used.26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69 Studies have demonstrated that iRoot BP Plus and MTA

have no significant differences in terms of their biocompatibility or sealing ability.70 However, iRoot BP Plus exhibits superior marginal adaptability,68 and in animal models of periapical

periodontitis and clinical trials, it has been shown to have a better healing effect than MTA.71 This result may be attributed to the anti-inflammatory properties of the nanoparticles in

iRoot BP Plus, such as calcium silicate. RESTORATION AND STABILIZATION OF THE AFFECTED TOOTH TOOTH REPLANTATION AND OCCLUSION ADJUSTMENT When a blood clot in the alveolar socket hinders

replantation, it can be gently removed with an aspirator, although direct contact with the alveolar socket should be avoided. The tooth should be reinserted into the alveolar socket with

digital pressure along the long axis of the tooth. A gauze ball is placed on the occlusal surface if replantation is restricted, and the patient is asked to gently bite the tooth together to

help the tooth settle in place.72 A 3 mm root-end resection generally leads to infraocclusion, so occlusal reduction is unnecessary. If there is premature contact with the affected tooth,

whether the affected tooth is fully in place should be assessed first, and then the tooth should be reinserted if necessary. All occlusal interference should be removed.10 When the tooth is

fully in place, the alveolar socket is compressed in a buccolingual direction to better fit the root. The patient is asked to gently bite on gauze or a cotton ball for five minutes to

stabilize the affected tooth in cases with good periodontal health and interradicular bone intervals. STABILIZATION TECHNIQUE FOR THE AFFECTED TOOTH The requirement and duration of

stabilization depend on the mobility of the affected tooth after replantation. Currently, a periodontal splint is recommended for teeth with short roots or loss of alveolar bone when the

degree of tooth mobility is II or III. However, this approach is not recommended for teeth with a degree of mobility of I. The ESE guidelines state that periodontal splints help promote

healing of the periodontium of the affected tooth, and fine steel wires no greater than 0.3–0.4 mm in length should be bonded to the labial surface for stabilization.10 The duration of

splinting varies from two weeks for most patients to up to 6 weeks for teeth with high mobility after surgical extrusion. Some researchers have raised concerns that the use of periodontal

splints with steel wires may affect physiologic tooth mobility and lead to ankylosis. In such cases, elastic fixation is recommended. This procedure involves a resin-bonded glass span seated

in a prepared microgroove on the occlusal surface of the affected tooth and a dovetail on the adjacent tooth.73 This elastic splint can be maintained for more than a year without affecting

normal physiological tooth mobility. ADDITIONAL MANAGEMENT BEFORE REPLANTATION Treating the root surface with specific drugs can improve periodontal reconstruction. Enamel matrix derivative

(EMD) is a commercial product that contains 90% enamel matrix protein and promotes the reattachment of periodontal tissue, including the periodontal ligament, acellular cementum layer, and

alveolar bone.74,75,76 Studies have shown that EMD can also decrease the survival of odontoclasts on the root surface and lower the risk of root resorption.77 The application of EMD

significantly affects the success rate of intentional replantation.65 Radiographic examination revealed that root surfaces treated with tetracycline hydrochloride solution for five minutes

and saline irrigation are free from root resorption.78 Some studies have found that preoperative treatment with minocycline hydrochloride promotes the regeneration of perforating fibers,

which may be attributed to the antibacterial and anti-resorptive effects of tetracycline drugs on bone tissue.78 Overall, these laboratory and clinical studies verified that tetracyclines

have antibacterial effects, promote alveolar bone formation and inhibit root resorption after replantation. Some studies have demonstrated that pretreatment with enriched platelet products

can also improve periodontal regeneration. Autologous platelet-rich plasma used for socket medicament of teeth affected with severe periodontitis can promote alveolar bone regeneration.79

The preoperative use of platelet-rich fibrin covered with a collagen membrane in a central incisor with severe periodontitis increased bone mass by approximately 87% at the one-year

follow-up.80 Some reports have shown that platelet-rich fibrin can promote the regeneration of periodontal tissue and prevent postoperative ankylosis.81 TREATMENT OF SPECIAL CASES VERTICAL

ROOT FRACTURES In ITR cases where vertical fractures are restricted in the root cross-section, sealing the fractures with resin cement extraorally is recommended to preserve the

tooth.82,83,84 Moderate drying is performed to enhance the bonding effect of resin cement. Vertical root fractures involve the destruction of dentin, cementum, and the periodontal ligament.

Resin can physically block communication between the pulp cavity and periodontium by sealing the fracture line without forming new cementum or periodontal ligament, which does not indicate

true regeneration of dental and periodontal tissue. Fortunately, the development of bioactive materials such as CSC has improved the restoration of vertical root fractures. The microhardness

and modulus of elasticity of Biodentine closely resemble those of natural dentin, making it an ideal fracture repair material. Furthermore, the initial setting time of Biodentine is only 15

 minutes, and the final setting time is 45 minutes, resulting in a shorter extraoral operation period than that of MTA.85 Additionally, inorganic ions such as Ca/Si released from Biodentine

create a beneficial environment for the attachment, proliferation, and differentiation of periodontal ligament stem cells, promoting biomineralization and the formation of new periodontal

attachments.86 Many studies have shown the promising effect of ITR in treating vertical root fractures, but these studies are limited to individual case reports. A retrospective study

revealed that the 12-month and 24-month survival rates of teeth with vertical root fractures after ITR were 83.3% and 36.3%, respectively.87 CERVICAL RESORPTION LESIONS Cervical resorption,

which involves the loss of hard tooth tissue at the cementoenamel junction due to various factors, poses a challenge in avoiding resorption progression and achieving functional and

aesthetically pleasing restoration. Intraoral conditions limit the field of vision and operating space, making it difficult to fully expose the lesion area. Periodontal flap surgery is often

necessary but can cause further damage. ITR allows for direct visualization of the entire tooth, enabling treatment and a more definite prognosis.48 Treatment involves mechanical curettage

and chemical debridement to remove granulation tissue and irregular parts of the absorption interface, exposing healthy dentin. The choice of repair material depends on the lesion location.

For lesions located coronal to the cementoenamel junction, resin cement or glass ionomer is recommended, while CSC is recommended for apical lesions. Biodentine is a promising material for

repairing dental tissue defects due to its mechanical properties that closely resemble those of natural dentin, faster setting, and superior biocompatibility and bioregulation, as mentioned

above.88 POSTOPERATIVE MANAGEMENT AND FOLLOW-UP In the early stages following ITR, the periodontal tissue of the replanted tooth is in an open traumatic state, requiring consistent

maintenance of oral hygiene. Apart from the replanted tooth and its adjacent teeth, routine cleaning should be performed on all other teeth. Typically, within the first week postoperatively,

mouth rinses with 0.12% chlorhexidine solution should be used 2–3 times daily. A soft diet and avoiding chewing on the replanted tooth are recommended during the first week after surgery.

Standard pain management protocols can be employed postoperatively, such as oral ibuprofen (300–600 mg/time, bid).88 The use of antibiotics postoperatively remains controversial. Some

studies suggest that systemic use of antibiotics postoperatively is beneficial for preventing infection, with patients not receiving antibiotics having a 1.4-fold greater probability of

developing complications such as root resorption.89 However, more studies and clinical guidelines advocate the cautious use of antibiotics.10 If an RCT is not performed prior to the ITR

procedure, it must be carried out within 2 weeks postoperatively. Restoration and orthodontic treatments may commence after 6 months once the replanted tooth stabilizes. DURATION AND CONTENT

OF FOLLOW-UP Follow-up appointments are recommended at 2 weeks, 1 month, 3 months, 6 months, and 1 year postoperatively, with the option of extending follow-up intervals if necessary.

During follow-up visits, any symptoms should be documented, and oral examination should include percussion, mobility, occlusion, palpation, periodontal probing, and assessment for sinus

tract or fistula formation. Radiographic imaging of the periapical area is mandatory. Importantly, periodontal probing should not be performed prematurely after surgery to avoid disrupting

the reconstruction of periodontal tissue.90 EVALUATION OF OUTCOMES SHORT-TERM OUTCOMES (1 WEEK AFTER SURGERY) * Improvement of preoperative clinical symptoms, including reduction or

disappearance of pain, and healing of the gingival fistula. * Assessment of periodontal tissue healing, including the ability to chew with the replanted tooth, pain or discomfort during

chewing, clinical examination of gingival inflammation, tooth mobility, sensitivity to percussion, and tenderness. * Detection of premature contacts between the replanted tooth and opposing

teeth. LONG-TERM OUTCOMES (6 MONTHS AFTER SURGERY AND BEYOND) * The absence of clinical symptoms, such as chewing pain and tooth mobility, and normal masticatory function. * Clinical

examination revealing the absence of gingival inflammation, significant recession, sensitivity to percussion, or tooth mobility. * Periapical X-rays showing healing of the periapical

lesions, absence of new lesions, and normalization of the periodontal ligament space. * No signs of root resorption or ankylosis. PROGNOSIS AND COMPLICATIONS The types of periodontal tissue

healing following ITR include periodontal ligament healing, surface resorption healing, inflammatory resorption, and replacement resorption.63 (1) Periodontal ligament healing is the best

outcome that frequently occurs after immediate replantation and involves either reattachments or new attachments, both of which rely on the presence of surviving periodontal ligament cells

on the root surface and within the alveolar

socket.38,39,40,41,42,43,44,45,46,47,48,49,50,51,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93 (2) Surface resorption

healing occurs when there is limited damage to the root surface and periodontal ligament, and healing occurs after repair by adjacent intact periodontal ligaments. Pure surface resorption is

self-limiting and can be repaired by the formation of new cementum and periodontal ligaments, resulting in new reattachment. (3) Inflammatory resorption occurs when inflammation persists

after tooth replantation, leading to significant resorption of alveolar bone and progressive root resorption. The rapid progression of inflammatory resorption can result in the failure of

ITR. A meta-analysis revealed that among 838 replanted teeth, the overall success rate was 88%, with 11% failing due to root resorption, which was the primary cause of replantation

failure.94 (4) Tooth ankylosis and replacement resorption occur when severe damage to the root surface or periodontal ligament leads to direct contact between the root and alveolar bone,

resulting in fusion and bone repair and characterized clinically by the absence of tooth mobility and radiographically by the disappearance of the periodontal ligament space. Prolonged

extraoral time, root desiccation, and reduced vitality of periodontal ligament cells can impact tissue healing, leading to complications such as external root resorption, root fusion, and

postoperative infection.95 Additionally, factors such as the extent of periapical lesions, periodontal condition, patient age, level of expertise in extraction technique, alveolar socket

preparation, and choice of root-end filling materials can affect the prognosis of replantation.43,45,46,47,48,49,50,51,55,56,57,58,59,60,61,62,63,64,65 Recent systematic reviews and

meta-analyses indicate an overall average survival rate of approximately 88% for ITR,96 with the success rate varying over time. The short-term success rate (< 6 months) is approximately

90%, decreasing to 65% to 80% at 12 to 36 months and stabilizing beyond 36 months of follow-up.57 Asymptomatic replanted teeth may continue to be observed, and their impact on subsequent

alternative implant treatment planning should be considered. CONCLUSION AND EXPECTATIONS As a last resort for preserving teeth with periapical lesions, ITR provides a viable option for

maximizing the retention of natural teeth. The affected teeth are generally brittle and are susceptible to fracture during the process of extraction, therefore, endodontists should be

well-versed in the indications and clinical management of this technique to enhance its long-term success. It is worth noting that current research on ITR mainly relies on case reports and

retrospective studies, and there is a lack of high-level evidence such as randomized controlled trials. In order to obtain more convincing conclusions, further studies with larger sample

sizes and longer observation periods are needed. Meanwhile, it is necessary to explore the key factors that affect the treatment outcomes and develop artificial intelligence evaluation

systems, so as to improve the success rate and predictability of ITR. REFERENCES * Fernandez, R., Cardona, J. A., Cadavid, D., Alvarez, L. G. & Restrepo, F. A. Survival of Endodontically

Treated Roots/Teeth Based on Periapical Health and Retention: A 10-year Retrospective Cohort Study. _J. Endod._ 43, 2001–2008 (2017). Article  PubMed  Google Scholar  * Chybowski, E. A. et

al. Clinical Outcome of Non-Surgical Root Canal Treatment Using a Single-cone Technique with Endosequence Bioceramic Sealer: A Retrospective Analysis. _J. Endod._ 44, 941–945 (2018). Article

  PubMed  Google Scholar  * Rouhani, A., Javidi, B., Habibi, M. & Jafarzadeh, H. Intentional replantation: a procedure as a last resort. _J. Contemp. Dent. Pr._ 12, 486–492 (2011).

Article  Google Scholar  * Grossman, L. I. Intentional replantation of teeth: a clinical evaluation. _J. Am. Dent. Assoc._ 104, 633–639 (1982). Article  PubMed  CAS  Google Scholar  *

Mainkar, A. A Systematic Review of the Survival of Teeth Intentionally Replanted with a Modern Technique and Cost-effectiveness Compared with Single-tooth Implants. _J. Endod._ 43, 1963–1968

(2017). Article  PubMed  Google Scholar  * Choi, Y. H. et al. Clinical outcome of intentional replantation with preoperative orthodontic extrusion: a retrospective study. _Int. Endod. J._

47, 1168–1176 (2014). Article  PubMed  CAS  Google Scholar  * Pisano, M. et al. Intentional Replantation of Single-Rooted and Multi-Rooted Teeth: A Systematic Review. _Healthcare (Basel)_ 11

(2022). * Praveen, K. V. et al. Current Trends in Intentional Replantation Treatment Among Endodontists and Postgraduate Students in India, the United States of America, and the United

Kingdom: A Cross-Sectional Study. _Cureus_ 15, e39742 (2023). Google Scholar  * Plotino, G., Abella, S. F., Bastos, J. V. & Nagendrababu, V. Effectiveness of intentional replantation in

managing teeth with apical periodontitis: A systematic review. _Int. Endod. J._ 56, 499–509 (2023). Article  PubMed  Google Scholar  * Plotino, G. et al. European Society of Endodontology

position statement: Surgical extrusion, intentional replantation and tooth autotransplantation. _Int. Endod. J._ 54, 655–659 (2021). Article  PubMed  CAS  Google Scholar  * Huang, D. M., Li,

J. Y. & Xu, X. [Clinical management of the intentional tooth replantation]. _Zhonghua Kou Qiang Yi Xue Za Zhi_ 53, 392–397 (2018). PubMed  CAS  Google Scholar  * Han-Yi, Z. &

Zheng-Wei, H. Clinical considerations for intentional replantation of teeth. _Chin. J. Practical Stomatol._ 14, 275–281 (2021). Google Scholar  * Wei, C. & Zhi-Fang, C. Research progress

of intentional replantation for periodontally involved teeth. _Beijing J. Stomatol._ 30, 296–299 (2022). Google Scholar  * Tingting, G., Jianhua, L. & Yunxia, W. Research Status of

Prognosis Factors of Intentional Tooth Replantation. _Med. Recapitulate_ 26, 4684–4688 (2020). Google Scholar  * Shumin, T. & Huiming, W. Research progress of intentional replantation.

_Electron. J. Gen. Stomatol._ 6, 8–10 (2019). Google Scholar  * Kai-Da, C. & Ming, X. Reasons for failure of root canal therapy and the diagnosis and managementstrategy of retreatment.

_Chin. J. Practical Stomatol._ 12, 201–207 (2019). Google Scholar  * Plotino, G. et al. Clinical procedures and outcome of surgical extrusion, intentional replantation and tooth

autotransplantation - a narrative review. _Int. Endod. J._ 53, 1636–1652 (2020). Article  PubMed  CAS  Google Scholar  * Kim, S. & Kratchman, S. Modern endodontic surgery concepts and

practice: a review. _J. Endod._ 32, 601–623 (2006). Article  PubMed  Google Scholar  * Emmertsen, E. & Andreasen, J. O. Replantation of extracted molars. A radiographic and histological

study. _Acta Odontol. Scand._ 24, 327–346 (1966). Article  PubMed  CAS  Google Scholar  * Wang, L. et al. Characterization of stem cells from alveolar periodontal ligament. _Tissue Eng. Part

A_ 17, 1015–1026 (2011). Article  PubMed  CAS  Google Scholar  * Wang, X. et al. Clinical application of calcium silicate-based bioceramics in endodontics. _J. Transl. Med._ 21, 853 (2023).

Article  PubMed  PubMed Central  CAS  Google Scholar  * Sanz, J. L., Guerrero-Girones, J., Pecci-Lloret, M. P., Pecci-Lloret, M. R. & Melo, M. Biological interactions between calcium

silicate-based endodontic biomaterials and periodontal ligament stem cells: A systematic review of in vitro studies. _Int. Endod. J._ 54, 2025–2043 (2021). Article  PubMed  Google Scholar  *

Harinkhere, C. et al. Comparison of the sealing ability amongst orthograde apical plugs of mineral trioxide aggregate plus, mineral trioxide aggregate repair HP, and Biodentine after root

resection: a bacterial leakage study. _Odontology_ 112, 364–371 (2024). Article  PubMed  CAS  Google Scholar  * Wang, Y. et al. Mineral trioxide aggregate enhances the osteogenic capacity of

periodontal ligament stem cells via NF-kappaB and MAPK signaling pathways. _J. Cell. Physiol._ 233, 2386–2397 (2018). Article  PubMed  CAS  Google Scholar  * Lu, J. et al. iRoot BP Plus

promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy. _Stem Cell Res. Ther._ 10, 222 (2019). Article  PubMed  PubMed Central 

Google Scholar  * Chen, I. et al. Healing after root-end microsurgery by using mineral trioxide aggregate and a new calcium silicate-based bioceramic material as root-end filling materials

in dogs. _J. Endod._ 41, 389–399 (2015). Article  PubMed  PubMed Central  Google Scholar  * Viswanath, G., Tilakchand, M., Naik, B. D., Kalabhavi, A. S. & Kulkarni, R. D. Comparative

evaluation of antimicrobial and antifungal efficacy of bioactive root-end filling materials: An in vitro study. _J. Conserv Dent._ 24, 148–152 (2021). Article  PubMed  PubMed Central  Google

Scholar  * Ravindran, V. & Jeevanandan, G. Comparative Evaluation of the Physical and Antimicrobial Properties of Mineral Trioxide Aggregate, Biodentine, and a Modified Fast-Setting

Mineral Trioxide Aggregate Without Tricalcium Aluminate: An In Vitro Study. _Cureus_ 15, e42856 (2023). PubMed  PubMed Central  Google Scholar  * Morgental, R. D. et al. Antibacterial

activity of two MTA-based root canal sealers. _Int. Endod. J._ 44, 1128–1133 (2011). Article  PubMed  CAS  Google Scholar  * Ashi, T. et al. Physicochemical and Antibacterial Properties of

Bioactive Retrograde Filling Materials. _Bioengineering (Basel)_ 9 (2022). * Liu, M., He, L., Wang, H., Su, W. & Li, H. Comparison of in vitro biocompatibility and antibacterial activity

of two calcium silicate-based materials. _J. Mater. Sci. Mater. Med_ 32, 52 (2021). Article  PubMed  PubMed Central  CAS  Google Scholar  * Kim, D., Ha, J. H., Jin, M. U., Kim, Y. K. &

Kim, S. K. Proximity of the mandibular molar root apex from the buccal bone surface: a cone-beam computed tomographic study. _Restor. Dent. Endod._ 41, 182–188 (2016). Article  PubMed 

PubMed Central  Google Scholar  * Wang, Y., Hofmann, M., Ruf, S., Zhang, J. & Huang, Q. Intentional replantation and dental autotransplantation of mandibular posterior teeth: Two case

reports. _Med. (Baltim.)_ 102, e35822 (2023). Article  Google Scholar  * Mainkar, A., Zhu, Q. & Safavi, K. Incidence of Altered Sensation after Mandibular Premolar and Molar Periapical

Surgery. _J. Endod._ 46, 29–33 (2020). Article  PubMed  Google Scholar  * Santos, J. M. et al. Intentional Replantation as a Starting Approach for a Multidisciplinary Treatment of a

Mandibular Second Molar: A Case Report. _J. Clin. Med._ 11 (2022). * Pruthi, P. J., Dharmani, U., Roongta, R. & Talwar, S. Management of external perforating root resorption by

intentional replantation followed by Biodentine restoration. _Dent. Res. J. (Isfahan)_ 12, 488–493 (2015). Article  PubMed  Google Scholar  * Asgary, S. & Talebzadeh, B. Intentional

replantation of a molar with several endodontic complications. _J. Stomatol Oral. Maxillofac. Surg._ 120, 489–492 (2019). Article  PubMed  Google Scholar  * Garrido, I., Abella, F.,

Ordinola-Zapata, R., Duran-Sindreu, F. & Roig, M. Combined Endodontic Therapy and Intentional Replantation for the Treatment of Palatogingival Groove. _J. Endod._ 42, 324–328 (2016).

Article  PubMed  Google Scholar  * Tan, X. et al. Palatal Radicular Groove Morphology of the Maxillary Incisors: A Case Series Report. _J. Endod._ 43, 827–833 (2017). Article  PubMed  Google

Scholar  * Hayashi, M., Kinomoto, Y., Takeshige, F. & Ebisu, S. Prognosis of intentional replantation of vertically fractured roots reconstructed with dentin-bonded resin. _J. Endod._

30, 145–148 (2004). Article  PubMed  Google Scholar  * Salihu, B. & Stavileci, M. Review of Approaches to the Management of Vertical Dental Root Fractures and Tooth Preservation. _Med.

Sci. Monit._ 30, e943100 (2024). Article  PubMed  PubMed Central  Google Scholar  * Peer, M. Intentional replantation - a ‘last resort’ treatment or a conventional treatment procedure? nine

case reports. _Dent. Traumatol._ 20, 48–55 (2004). Article  PubMed  Google Scholar  * Luo, Y. et al. Comparison of the outcome of intentional replantation in teeth with or without

periodontal involvement: a retrospective study. _J. Endod._ (2024). * Cho, S. Y., Lee, S. J. & Kim, E. Clinical Outcomes after Intentional Replantation of Periodontally Involved Teeth.

_J. Endod._ 43, 550–555 (2017). Article  PubMed  Google Scholar  * Javed, F., Zafar, K. & Khan, F. R. Outcome of intentional replantation of endodontically treated teeth with periapical

pathosis: A systematic review and meta-analysis. _Aust. Endod. J._ 49, 494–507 (2023). Article  PubMed  Google Scholar  * Caton, J. G. et al. A new classification scheme for periodontal and

peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. _J. Clin. Periodontol._ 45 SUPPL 20, S1–S8 (2018). PubMed  Google Scholar  * Becker, B. D.

Intentional Replantation Techniques: A Critical Review. _J. Endod._ 44, 14–21 (2018). Article  PubMed  Google Scholar  * Krug, R., Soliman, S. & Krastl, G. Intentional Replantation with

an Atraumatic Extraction System in Teeth with Extensive Cervical Resorption. _J. Endod._ 45, 1390–1396 (2019). Article  PubMed  Google Scholar  * Krug, R. et al. Surgical extrusion with an

atraumatic extraction system: A clinical study. _J. Prosthet. Dent._ 120, 879–885 (2018). Article  PubMed  Google Scholar  * Jang, Y., Lee, S. J., Yoon, T. C., Roh, B. D. & Kim, E.

Survival Rate of Teeth with a C-shaped Canal after Intentional Replantation: A Study of 41 Cases for up to 11 Years. _J. Endod._ 42, 1320–1325 (2016). Article  PubMed  Google Scholar  *

Nasjleti, C. E., Caffesse, R. G. & Castelli, W. A. Replantation of mature teeth without endodontics in monkeys. _J. Dent. Res._ 57, 650–658 (1978). Article  PubMed  CAS  Google Scholar 

* Moradi, M. N., Arvin, A., Darvish, A., Aflaki, S. & Homayouni, H. Treatment of necrotic calcified tooth using intentional replantation procedure. _Case Rep. Dent._ 2014, 793892 (2014).

Google Scholar  * Cunliffe, J., Ayub, K., Darcey, J. & Foster-Thomas, E. Intentional replantation - a clinical review of cases undertaken at a major UK dental school. _Brit. Dent. J._

229, 230–238 (2020). Article  PubMed  Google Scholar  * Cai, M., Xiang, S., Xie, C., Ouyang, C. & Tong, F. Intentional replantation for the retreatment of mandibular second molar: a case

report. _Hua Xi Kou Qiang Yi Xue Za Zhi_ 41, 471–477 (2023). PubMed  Google Scholar  * Grzanich, D., Rizzo, G. & Silva, R. M. Saving Natural Teeth: Intentional Replantation-Protocol and

Case Series. _J. Endod._ 43, 2119–2124 (2017). Article  PubMed  Google Scholar  * Pohl, Y., Filippi, A. & Kirschner, H. Results after replantation of avulsed permanent teeth. II.

Periodontal healing and the role of physiologic storage and antiresorptive-regenerative therapy. _Dent. Traumatol._ 21, 93–101 (2005). Article  PubMed  Google Scholar  * Wang, L. et al.

Clinical outcomes after intentional replantation of permanent teeth: A systematic review. _Bosn. J. Basic Med. Sci._ 20, 13–20 (2020). PubMed  PubMed Central  CAS  Google Scholar  * Hupp, J.

G., Mesaros, S. V., Aukhil, I. & Trope, M. Periodontal ligament vitality and histologic healing of teeth stored for extended periods before transplantation. _Endod. Dent. Traumatol._

14, 79–83 (1998). Article  PubMed  CAS  Google Scholar  * Levin, L., Bryson, E. C., Caplan, D. & Trope, M. Effect of topical alendronate on root resorption of dried replanted dog teeth.

_Dent. Traumatol._ 17, 120–126 (2001). Article  PubMed  CAS  Google Scholar  * Udoye, C. I., Jafarzadeh, H. & Abbott, P. V. Transport media for avulsed teeth: a review. _Aust. Endod. J._

38, 129–136 (2012). Article  PubMed  Google Scholar  * Osmanovic, A., Halilovic, S., Kurtovic-Kozaric, A. & Hadziabdic, N. Evaluation of periodontal ligament cell viability in different

storage media based on human PDL cell culture experiments-A systematic review. _Dent. Traumatol._ 34, 384–393 (2018). Article  PubMed  CAS  Google Scholar  * Liu, H., Peng, X., Sun, H.

& Li, X. Clinical and histopathological characterization of root resorption in replanted teeth: Two case reports. _Med. (Baltim.)_ 99, e18869 (2020). Article  Google Scholar  * Cho, S.

Y. et al. Retention and Healing Outcomes after Intentional Replantation. _J. Endod._ 42, 909–915 (2016). Article  PubMed  Google Scholar  * Deotale, S. P., Shewale, A. H., Gattani, D. R.,

Nathani, A. I. & Banode, A. M. Reliability of two different protocols used for intentional re-implantation of periodontally hopeless teeth. _J. Indian Soc. Periodontol._ 22, 353–359

(2018). Article  PubMed  PubMed Central  Google Scholar  * Wu, S. Y. & Chen, G. A long-term treatment outcome of intentional replantation in Taiwanese population. _J. Formos. Med.

Assoc._ 120, 346–353 (2021). Article  PubMed  Google Scholar  * Bodrumlu, E. Biocompatibility of retrograde root filling materials: a review. _Aust. Endod. J._ 34, 30–35 (2008). Article 

PubMed  Google Scholar  * Watts, J. D., Holt, D. M., Beeson, T. J., Kirkpatrick, T. C. & Rutledge, R. E. Effects of pH and mixing agents on the temporal setting of tooth-colored and gray

mineral trioxide aggregate. _J. Endod._ 33, 970–973 (2007). Article  PubMed  Google Scholar  * Shokouhinejad, N., Nekoofar, M. H., Ashoftehyazdi, K., Zahraee, S. & Khoshkhounejad, M.

Marginal adaptation of new bioceramic materials and mineral trioxide aggregate: a scanning electron microscopy study. _Iran. Endod. J._ 9, 144–148 (2014). PubMed  PubMed Central  Google

Scholar  * Willershausen, I. et al. Influence of a bioceramic root end material and mineral trioxide aggregates on fibroblasts and osteoblasts. _Arch. Oral. Biol._ 58, 1232–1237 (2013).

Article  PubMed  CAS  Google Scholar  * Jiang, Y., Zheng, Q., Zhou, X., Gao, Y. & Huang, D. A comparative study on root canal repair materials: a cytocompatibility assessment in L929 and

MG63 cells. _ScientificWorldJournal_ 2014, 463826 (2014). Article  PubMed  PubMed Central  Google Scholar  * Shinbori, N., Grama, A. M., Patel, Y., Woodmansey, K. & He, J. Clinical

outcome of endodontic microsurgery that uses EndoSequence BC root repair material as the root-end filling material. _J. Endod._ 41, 607–612 (2015). Article  PubMed  Google Scholar  *

Roudsari, R. V., Jawad, S., Taylor, C., Darcey, J. & Qualtrough, A. Modern Endodontic Principles. Part 8: The Future of Endodontics. _Dent. Update_ 43, 430-432, 435–438, 441 (2016).

Article  Google Scholar  * Kahler, B., Hu, J. Y., Marriot-Smith, C. S. & Heithersay, G. S. Splinting of teeth following trauma: a review and a new splinting recommendation. _Aust. Dent.

J._ 61, 59–73 (2016). Article  PubMed  Google Scholar  * Hirooka, H. The biologic concept for the use of enamel matrix protein: true periodontal regeneration. _Quintessence Int_ 29, 621–630

(1998). PubMed  CAS  Google Scholar  * Zeichner-David, M. Is there more to enamel matrix proteins than biomineralization? _Matrix Biol._ 20, 307–316 (2001). Article  PubMed  CAS  Google

Scholar  * Miron, R. J. et al. Twenty years of enamel matrix derivative: the past, the present and the future. _J. Clin. Periodontol._ 43, 668–683 (2016). Article  PubMed  Google Scholar  *

Wang, H. H. et al. Application of Enamel Matrix Derivative (Emdogain) in Endodontic Therapy: A Comprehensive Literature Review. _J. Endod._ 44, 1066–1079 (2018). Article  PubMed  Google

Scholar  * Demiralp, B., Nohutcu, R. M., Tepe, D. I. & Eratalay, K. Intentional replantation for periodontally involved hopeless teeth. _Dent. Traumatol._ 19, 45–51 (2003). Article 

PubMed  Google Scholar  * Tozum, T. F., Keceli, H. G., Serper, A. & Tuncel, B. Intentional replantation for a periodontally involved hopeless incisor by using autologous platelet-rich

plasma. _Oral. Surg. Oral. Med Oral. Pathol. Oral. Radio. Endod._ 101, e119–e124 (2006). Article  Google Scholar  * Ryana, H. K., Srinath, R. & Prakash, S. Surgical Re-entry of an

Intentionally Replanted Periodontally Compromised Tooth Treated with Platelet Rich Fibrin (PRF): Hopeless to Hopeful. _J. Clin. Diagn. Res_. 10, D1–D4 (2016). Google Scholar  * Deshpande, N.

M., Shah, D. & Wadekar, S. Maintenance of cell viability in extraoral conditions for a case of intentional replantation to retrieve a separated endodontic instrument. _J. Conserv Dent._

22, 207–212 (2019). Article  PubMed  PubMed Central  Google Scholar  * Kawai, K. & Masaka, N. Vertical root fracture treated by bonding fragments and rotational replantation. _Dent.

Traumatol._ 18, 42–45 (2002). Article  PubMed  Google Scholar  * Okaguchi, M., Kuo, T. & Ho, Y. C. Successful treatment of vertical root fracture through intentional replantation and

root fragment bonding with 4-META/MMA-TBB resin. _J. Formos. Med. Assoc._ 118, 671–678 (2019). Article  PubMed  Google Scholar  * Ozer, S. Y., Unlu, G. & Deger, Y. Diagnosis and

treatment of endodontically treated teeth with vertical root fracture: three case reports with two-year follow-up. _J. Endod._ 37, 97–102 (2011). Article  PubMed  Google Scholar  *

Hadrossek, P. H. & Dammaschke, T. New treatment option for an incomplete vertical root fracture-a preliminary case report. _Head. Face Med_. 10, 9 (2014). Article  PubMed  PubMed Central

  Google Scholar  * Dawood, A. E. et al. The physical properties and ion release of CPP-ACP-modified calcium silicate-based cements. _Aust. Dent. J._ 60, 434–444 (2015). Article  PubMed  CAS

  Google Scholar  * Hayashi, M. et al. Short-term evaluation of intentional replantation of vertically fractured roots reconstructed with dentin-bonded resin. _J. Endod._ 28, 120–124 (2002).

Article  PubMed  Google Scholar  * Rajasekharan, S., Martens, L. C., Cauwels, R. & Anthonappa, R. P. Biodentine material characteristics and clinical applications: a 3-year literature

review and update. _Eur. Arch. Paediatr. Dent._ 19, 1–22 (2018). Article  PubMed  CAS  Google Scholar  * Chung, W. C., Tu, Y. K., Lin, Y. H. & Lu, H. K. Outcomes of autotransplanted

teeth with complete root formation: a systematic review and meta-analysis. _J. Clin. Periodontol._ 41, 412–423 (2014). Article  PubMed  Google Scholar  * Hatakeyama, Y., Uzel, M. I.,

Santana, R. B. & Ruben, M. P. Relationship between position of probe tip and periodontal tissues after periodontal surgery in dogs. _Int J. Periodontics Restor. Dent._ 25, 247–255

(2005). Google Scholar  * Wang, F., Li, Q. & Wang, Z. A comparative study of the effect of Bio-Oss((R)) in combination with concentrated growth factors or bone marrow-derived mesenchymal

stem cells in canine sinus grafting. _J. Oral. Pathol. Med._ 46, 528–536 (2017). Article  PubMed  CAS  Google Scholar  * Zanjir, M. et al. Efficacy and Safety of Postoperative Medications

in Reducing Pain after Nonsurgical Endodontic Treatment: A Systematic Review and Network Meta-analysis. _J. Endod._ 46, 1387–1402 (2020). Article  PubMed  Google Scholar  * Nizam, N., Kaval,

M. E., Gurlek, O., Atila, A. & Caliskan, M. K. Intentional replantation of adhesively reattached vertically fractured maxillary single-rooted teeth. _Int. Endod. J._ 49, 227–236 (2016).

Article  PubMed  CAS  Google Scholar  * Torabinejad, M. et al. Survival of Intentionally Replanted Teeth and Implant-supported Single Crowns: A Systematic Review. _J. Endod._ 41, 992–998

(2015). Article  PubMed  Google Scholar  * Weine, F. S. The case against intentional replantation. _J. Am. Dent. Assoc._ 100, 664–668 (1980). Article  PubMed  CAS  Google Scholar  * Chogle,

S., Chatha, N. & Bukhari, S. Intentional Replantation of Teeth is a Viable and Cost-effective Alternative Treatment to Single-Tooth Implants. _J. Evid. Based Dent. Pr._ 19, 86–88 (2019).

Article  Google Scholar  Download references AUTHOR INFORMATION Author notes * These authors contributed equally: Zhengmei Lin, Dingming Huang, Shuheng Huang AUTHORS AND AFFILIATIONS *

Department of Operative Dentistry and Endodontics, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University & Guangdong Provincial Key Laboratory of Stomatology,

Guangzhou, China Zhengmei Lin, Shuheng Huang & Junqi Ling * State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology

and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China Dingming Huang, Jiyao Li, Lan Zhang & Xuedong Zhou * State Key Laboratory of Oral &

Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan

University, Wuhan, China Zhi Chen * State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shanxi Key

Laboratory of Oral Diseases, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, China Qing Yu * Center for Microscope

Enhanced Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China Benxiang Hou * Department of Endodontics, School of Stomatology, China Medical University,

Shenyang, China Lihong Qiu * College & Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China Wenxia Chen * Department of Cariology and Endodontology, Peking

University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research of Oral Biomaterials and Digital Medical Devices

& Beijing Key Laboratory of Digital Stomatology, Peking University, Beijing, China Xiaoyan Wang & Lin Yue * Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao

Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Clinical Research Center for Oral Diseases, National Center for Stomatology, Shanghai Key

Laboratory of Stomatology, Shanghai, China Zhengwei Huang * Department of Endodontics, Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical

University, Nanjing, China Jinhua Yu * Department of Endodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China Jin Zhao * Department of Endodontics, School and

Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China Yihuai Pan * Department of Endodontics, The First Affiliated Hospital of Harbin Medical University & Department of

Endodontics, School of Stomatology, Harbin Medical University, Harbin, China Shuang Pan * Department of Conservative Dentistry and Endodontics, Shanghai Stomatological Hospital & School

of Stomatology, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China Deqin Yang * School of Stomatology, Dalian Medical University,

Dalian, Liaoning, China Weidong Niu * Department of Endodontics, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and

Regeneration, Shanghai, China Qi Zhang * Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases,

Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, China Shuli Deng * Department of Stomatology, Union Hospital, Tongji Medical

College, Huazhong University of Science and Technology, Wuhan, China Jingzhi Ma * Hospital of Stomatology, Jilin University, Changchun, China Xiuping Meng * Department of Endodontics, The

Affiliated Stomatological Hospital of Nanchang University, Nanchang, China Jian Yang * Key Laboratory of Oral Disease Research, School of Stomatology, Zunyi Medical University, Zunyi, China

Jiayuan Wu * Shandong University, School of Stomatology, Jinan, Shandong, China Jin Zhang * Department of Endodontology, Hunan Xiangya Stomatological Hospital, Central South University,

Changsha, China Xiaoli Xie * The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China Jinpu Chu * Department of Endodontics, College of Stomatology, Tianjin Medical

University, Tianjin, China Kehua Que * Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan,

Shanxi, China Xuejun Ge * Fujian Key Laboratory of Oral Disease & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and

University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China Xiaojing Huang * Department of Preventive Dentistry, Hebei Key Laboratory of Stomatology, Hebei

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L.Z., J.Z., X.X., J.C., K.Q., X.G., X.H., Z.M. CORRESPONDING AUTHORS Correspondence to Lin Yue, Xuedong Zhou or Junqi Ling. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no

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CITE THIS ARTICLE Lin, Z., Huang, D., Huang, S. _et al._ Expert consensus on intentional tooth replantation. _Int J Oral Sci_ 17, 16 (2025). https://doi.org/10.1038/s41368-024-00337-5

Download citation * Received: 14 May 2024 * Revised: 08 November 2024 * Accepted: 08 November 2024 * Published: 03 March 2025 * DOI: https://doi.org/10.1038/s41368-024-00337-5 SHARE THIS

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