ABSTRACT Pancreatic neoplasms, including pancreatic ductal adenocarcinoma (PDAC), intraductal papillary mucinous neoplasm (IPMN) and pancreatic cystic neoplasms (PCNs), are the most puzzling

diseases. Numerous studies have not brought significant improvements in prognosis and diagnosis, especially in PDAC. One important reason is that previous studies only focused on

differences between patients and healthy individuals but ignored intratumoral heterogeneity. In recent years, single-cell sequencing techniques, represented by single-cell RNA sequencing

(scRNA-seq), have emerged by which researchers can analyse each cell in tumours instead of their average levels. Herein, we summarise the new current knowledge of single-cell sequencing in

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HETEROGENEITY, PLASTICITY AND RESPONSE TO THERAPY Article 23 March 2023 SINGLE-CELL PROFILING REVEALS DIFFERENCES BETWEEN HUMAN CLASSICAL ADENOCARCINOMA AND MUCINOUS ADENOCARCINOMA Article

Open access 23 January 2023 TUMOR ORGANOIDS IMPROVE MUTATION DETECTION OF PANCREATIC DUCTAL ADENOCARCINOMA Article Open access 26 October 2024 DATA AVAILABILITY Not applicable. REFERENCES *

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https://doi.org/10.1038/s41419-020-02787-1. Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS The authors thank Dr. Le Li for assistance with his comments and proof reading

that greatly improved the manuscript. This paper was supported by grants from the National Natural Scientific Foundation of China (81770639,82070657), Applied technology research and

development project of Heilongjiang Province (GA20C019), Outstanding youth funds of the first affiliated hospital of Harbin Medical University (HYD2020JQ0006), and Research projects of

Chinese Research Hospital Association (Y2019FH-DTCC-SB1). AUTHOR INFORMATION Author notes * These authors contributed equally: Gaoyuan Lv, Liang Zhang. AUTHORS AND AFFILIATIONS * Department

of Pancreatic and Biliary Surgery, The First Affiliated Hospital of Harbin Medical University, 150000, Harbin, Heilongjiang Province, China Gaoyuan Lv, Liang Zhang, Lei Gao, Jitao Cui, 

Ziying Liu, Bei Sun & Gang Wang * Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, China Gaoyuan Lv 

& Gang Wang * Chinese Journal of Practical Surgery, Chinese Medical University, Shenyang, China Qiushi Tang Authors * Gaoyuan Lv View author publications You can also search for this

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permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Lv, G., Zhang, L., Gao, L. _et al._ The application of single-cell sequencing in pancreatic neoplasm: analysis, diagnosis and treatment. _Br

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