Metabolomic insights into the arabica-like flavour of stenophylla coffee and the chemistry of quality coffee

Metabolomic insights into the arabica-like flavour of stenophylla coffee and the chemistry of quality coffee

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ABSTRACT Stenophylla coffee, an undomesticated species from Upper West Africa, is of commercial interest due to its high heat tolerance and Arabica-like flavour. To investigate the chemical


basis of flavour similarity, we analysed unroasted coffee bean samples using liquid chromatography–mass spectrometry (LC–MS) and applied metabolomics approaches to compare chemical profiles.


We report similarities between Arabica and stenophylla in the relative levels of several key compounds linked to coffee flavour, including caffeine, trigonelline, sucrose and citric acid.


Differences in their chemical profiles were also observed, especially in their diterpenoid and hydroxycinnamic acid profiles. We report the additional novel finding that theacrine occurs in


stenophylla, which is the first record of this alkaloid in coffee beans. For stenophylla, the dissimilarities in chemical compound composition (compared to Arabica) may offer opportunities


for a better understanding of the chemical basis of high-quality coffee and sensory diversification. SIMILAR CONTENT BEING VIEWED BY OTHERS GC-MS BASED NUTRITIONAL AND AROMA PROFILING OF


DATE PALM SEEDS COLLECTED FROM DIFFERENT EGYPTIAN CULTIVARS FOR VALORIZATION PURPOSES Article Open access 13 May 2025 VOLATILE ORGANIC COMPOUNDS (VOCS) FINGERPRINTING COMBINED WITH COMPLEX


NETWORK ANALYSIS AS A FORECASTING TOOL FOR TRACING THE ORIGIN AND GENETIC LINEAGE OF ARABICA SPECIALTY COFFEES Article Open access 21 April 2025 INFLUENCE OF COFFEE BREWING METHODS ON THE


CHROMATOGRAPHIC AND SPECTROSCOPIC PROFILES, ANTIOXIDANT AND SENSORY PROPERTIES Article Open access 01 November 2021 INTRODUCTION Consumer demand supports a multi-billion-dollar coffee


sector. At least 80 countries produce coffee at scale, resulting in global exports of over 10 billion kg per year1. The appreciation of coffee is driven by sensory pleasure, its stimulatory


properties (mainly due to caffeine), and myriad cultural associations, but above all, a coffee-like flavour is required. For most coffee consumers, Arabica coffee (_Coffea arabica_) is the


first choice, and then robusta or conilon (_Coffea canephora_), which together comprise at least 99.9% of global coffee trade. Liberica (_Coffea liberica_) and excelsa (_Coffea dewevrei_)


are minor coffee crop species, although their popularity with farmers and consumers is experiencing a revival2. Other coffee species are farmed, but only at small scale with negligible


production volumes3. The long-term sustainability of the coffee farming sector is a major concern in an era of accelerated climate change4. A range of adaptation pathways have been


suggested3, but one of the most pressing requirements is to provide alternative coffee crop options for farmers that are no longer able to produce economically viable Arabica or robusta


coffee due to climate change5. Whereas improved Arabica and robusta variants may provide potential, switching coffee species entirely is likely to provide greater gains in terms of climate


change resiliency3,5. Key coffee species candidates include stenophylla (_Coffea stenophylla_)3,6, Liberica (_C. liberica_)2, and excelsa (_C. dewevrei_)2,7,8. Stenophylla coffee has been


the subject of intense interest, due to its high-quality Arabica-like flavour3,9. This is remarkable because stenophylla and Arabica are neither closely related phylogenetically10,11, nor


similar morphologically3. Moreover, their indigenous distributions and climate envelopes do not overlap3. Arabica is a cool-tropical species, indigenous to the highland forests of Ethiopia


and South Sudan at an elevation of 1000–2200 m12,13,14; stenophylla occurs in the lowland forests of Guinea, Sierra Leone, Ivory Coast and possibly Liberia6 at c. 400 m. Over its indigenous


range, Arabica receives c. 1600 mm of rainfall per year and a mean annual temperature of c. 18.7 °C3, and stenophylla 1500–2288 mm and 25–26 °C3,15. Naturally occurring stenophylla has a


substantially higher heat tolerance compared to Arabica (mean annual temperature 6.2–6.8 °C higher) even under similar rainfall conditions3. Among the approximately 130 _Coffea_ species5,16,


stenophylla is the only species known to have an Arabica-like flavour (other than Arabica itself)3,9. Indeed, the cup profile and flavour of stenophylla coffee has even been considered as


indistinguishable to specific regional variants of Arabica, and particularly high elevation, Rwanda _C. arabica_ ‘Bourbon’3. This is compelling given the phylogenetic, geographical, and


environmental differences between the two species. The common perception for paramount coffee quality is that it should be Arabica, grown on farms at high elevations (e.g., 1600 m or more),


which experience cool-tropical temperatures with a wide diurnal variation (i.e., considerable difference between day and night temperatures), and high UV levels17,18,19. Thus, stenophylla


breaks the orthodoxy for fundamental quality coffee requirements. Given the global demand for the Arabica flavour profile, the threat posed by climate change to Arabica4,20, and the


incongruity between perceived genetic and environmental parameters for high-quality coffee, understanding the chemical relationships between Arabica and stenophylla is a key aim for coffee


sensory research. The chemistry underpinning coffee flavour and quality is highly complex. More than 700 compounds (in ‘green’, unroasted coffee) have been considered to influence coffee


flavour and aroma: key compounds modulating flavour and quality include caffeine, trigonelline, sugars, hydroxycinnamic (including chlorogenic) acids, and other acids21,22,23. Moreover,


coffee chemistry, and hence flavour, may be influenced by many other factors including the species or cultivar (‘variety’), geographic origin of the beans, climate factors17,18,19,24, and


post-harvest processing methods22. Roasting also substantially influences coffee chemistry, due to the complex conversion of many compounds (discussed here) by processes such as the Maillard


reaction, caramelisation and the production of numerous volatiles via pyrolysis25. Regardless of these variables, green bean coffee chemistry can be correlated with the sensory quality of


brewed coffee22,26 and used to characterise _Coffea_ species27,28,29,30. In this article, we elucidate the chemical profile of green (unroasted) stenophylla coffee beans using liquid


chromatography–mass spectrometry (LC–MS) and metabolomics approaches to understand the chemical relationships between this poorly-studied species and Arabica coffee. For comparison, we


include the other major commercial coffee species, robusta, as many differences in green coffee chemistry between Arabica and robusta have been reported31. We focus on the comparison of


compounds that are considered to be important for coffee flavour, including alkaloids such as caffeine and trigonelline, hydroxycinnamic acid derivatives including chlorogenic acids, other


acidic compounds, sucrose and diterpenoids. Principal component analysis (PCA) and hierarchical clustering are used to assess the differences in the overall metabolomic profiles between the


three species. METHODS SAMPLE SELECTION Seeds (beans) of 26 coffee accessions were sampled for chemical analysis (Supplementary Table S4). The Arabica samples include five representatives of


indigenous Ethiopian cultigens, and six randomly selected from other cultivated sources (El Salvador, Brazil, Colombia, Rwanda, and Indonesia), four of which are well-known, named


cultivars. The eight robusta samples represent a random selection from cultivated stock, from Brazil, Rwanda, Uganda, India (×2), Indonesia (×2). The seven stenophylla samples were collected


from wild trees in Sierra Leone. The sampling included sun-dried samples for all species, and a few random samples of semi-washed or washed samples for Arabica and robusta. Most green bean


compounds are stable regardless of processing methods although there can be quantitative differences22. LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS Each individual coffee bean was


ground using a pestle and mortar, prior to extraction of the powdered material at a concentration of 100 mg/ml in 80:20 methanol:water at room temperature for 24 h, prior to centrifugation


and transfer of supernatants to LC-MS vials. Three individual beans were analysed for each accession (i.e., analyses were in triplicate per accession). Supernatants were analysed using a


Thermo Scientific LC–MS system consisting of a ‘Vanquish Flex’ U-HPLC-PDA, and an ‘Orbitrap Fusion’ mass spectrometer fitted with an “Ion Max NG” heated electrospray source (Thermo


Scientific, Waltham, MA, USA). Chromatography was performed on 5 µl sample injections onto a 150 mm × 3 mm, 3 µm Luna C-18(2) column (Phenomenex, Torrance, CA, USA) using the following 400 


µl/min mobile phase gradient of H2O/CH3OH/CH3CN + 1% HCOOH: 90:0:10 (0 min), 0:90:10 (60 min), 0:90:10 (70 min), 90:0:10 (71 min), 90:0:10 (75 min). Solvents were obtained from Fisher


Scientific (OPTIMA LC-MS grade). The heated ESI source was operated under the manufacturer’s default conditions for the flow rate employed and the mass spectrometer was set to record high


resolution (60 k resolution) MS1 spectra (_m/z_ 125–1800) in both positive and negative modes using the orbitrap; and data dependent MS2 and MS3 spectra in both modes using the linear ion


trap. Detected compounds were assigned using the approach described by Schymanski et al.32 and were by comparison of accurate mass (ppm) and interpretation of available MSn and UV spectra,


with reference to Kew’s in-house libraries of ion trap MS and UV spectra, in addition to comparison with published data33,34,35,36. The assignments of trigonelline, caffeine, (Sigma-Aldrich)


and theacrine (PhytoLab, PhytoProof grade) were also by comparison with reference standards. Quality control samples (consisting of the pooled coffee extracts analysed) were analysed every


ten samples to monitor and determine LC–MS performance and stability throughout the analysis. DATA PROCESSING AND CHEMOMETRICS LC–MS data were processed with Compound Discover v3.1 (Thermo


Scientific, USA) to obtain peak areas for each chromatogram. Chromatographic features were grouped into compounds if they had the same retention time and grouped feature areas were summed to


give compound peak areas for statistical analyses. Peak areas were used as a measure of the relative levels of the compounds detected in the species analysed. To verify differences between


species for each of the 37 assigned compounds, pairwise statistical tests were used. Where both samples passed normality tests (either _N_ > 30 or _p_ > 0.05 in the Shapiro–Wilk


test37) Welch’s two sample _t_-test38 was used, otherwise the non-parametric Mann–Whitney _U_ test was used39. To show equivalence for a given compound and pair of species, the two one-sided


test (TOST) procedure was used, with an effect size of 25% of the mean of the given samples. Again, where the samples pass normality tests we used Welch’s _t_-test as the one-sided test,


else we used a one-sided Mann–Whitney _U_ test. In both cases, Hochberg’s step-up procedure40 was implemented to correct for the family-wise error rate associated with multiple tests.


Statistical tests were carried out using the scipy41 and statsmodels42 Python libraries. The PCA was implemented using scikit-learn43 after scaling the values by removing the mean and


scaling to unit variance. PERMANOVA44, implemented in scikit-bio45, was used to verify the distinctions seen between species in the PCAs. Euclidean distance was used to generate the relevant


dissimilarity matrices, and 1000 permutations were used to assess statistical significance. The Logistic Regression models trained on the principal components and the evaluation procedure


were implemented using scikit-learn. For the hierarchical clustering heatmaps, values were first scaled by removing the mean and scaling to unit variance. The heatmap was implemented and


plotted in seaborn46, using Pearson correlation to measure distance and the complete linkage method to assign clusters. RESULTS COMPARISON OF COMPOUNDS BETWEEN STENOPHYLLA, ARABICA AND


ROBUSTA Representative base peak chromatograms (positive and negative ionisation modes) for each of the three species are shown in Fig. 1, to illustrate their chemical profiles. In total, 37


compounds were assigned across the three species (Fig. 1; Supplementary Tables S1–S3); the detection of these compounds is discussed below, with a focus on compounds associated with coffee


flavour. ALKALOIDS Caffeine was one of the major compounds detected by LC–MS analysis (peak 9, Fig. 1). Analysis of the relative quantities of caffeine in all three species (Fig. 2) show a


significantly higher occurrence in robusta coffee than in both Arabica (_P_ < 0.001), as previously observed47,48, and stenophylla (_P_ < 0.001). The caffeine contents of Arabica and


stenophylla were found to be similar (TOST _P_ = 0.077). Less intraspecific variation in caffeine was observed in stenophylla, which is perhaps due to the lack of variation in geographical


origin of the samples compared to the other two species. In addition to caffeine, stenophylla was found to contain the related alkaloid theacrine (7), which was not detected in any samples


of Arabica or robusta (_P_ < 0.001) (Fig. 2) and has not been reported in coffee beans previously. Comparison of the trigonelline (1) content showed no statistically significant


differences between the three species (Fig. 2), but Arabica and stenophylla were found to be similar through TOST (_P_ = 0.096). HYDROXYCINNAMIC ACIDS AND DERIVATIVES The chlorogenic acids


(3-, 4-, and 5-_O_-caffeoylquinic acids, peaks 6, 8, & 10) were amongst the main compounds detected in the three species analysed by LC–MS (Fig. 1); Fig. 2 shows the comparison of the


latter across the three species. No significant differences in the levels of these chlorogenic acids were found between Arabica and stenophylla (_P_ = 0.33, 0.99, and 0.58, respectively),


although robusta had a greater content of 3-_O_-caffeoylquinic acid than Arabica (_P_ < 0.001). 5-_O_-Feruloylquinic acid (13) was detected at higher levels in robusta than in the other


two species (_P_ < 0.001 for both). Though a difference in levels between Arabica and stenophylla was similarly observed (_P_ < 0.001), the magnitude of the difference was smaller by


comparison, Arabica only accumulating slightly more than stenophylla. Caffeoyl- and feruloyl-quinic acids have previously been reported to occur at higher levels in robusta, compared with


Arabica24,49. The amount of caffeic acid (11) was not found to differ significantly between Arabica and stenophylla (_P_ = 0.082, >0.1 following Hochberg correction), but were higher in


robusta (_P_ < 0.001 compared to both Arabica and stenophylla). The amount of 5-_O_-coumaroylquinic acid (12) in stenophylla was found to be significantly lower than in Arabica (_P_ < 


0.001) and robusta (_P_ < 0.001). A number of doubly-esterified quinic acids were also detected by LC–MS: 3,4- and 4,5-di-_O_-caffeoylquinic acid (22 & 24),


4-_O_-caffeoyl-3-_O_-feruloylquinic acid (25), caffeoylvaleroylquinic acid (29), two compounds assigned as dimethoy-cinnamoylcaffeoylquinic acids (28 & 32), and one assigned as


dimethoxy-cinnamoylferuloylquinic acid (35). Of these, 4,5-di-_O_-caffeoylquinic acid and 4-_O_-caffeoyl-3-_O_-feruloylquinic acid (Fig. 2) were detected in higher amounts in robusta


compared to Arabica (_P_ < 0.001 for both) and stenophylla (_P_ < 0.001 for both). A statistically significant difference in levels of these compounds between Arabica and stenophylla


was not observed. However, a lower content of 3,4-di-_O_-caffeoylquinic acid was observed in stenophylla, compared to the other species analysed (_P_ < 0.001 for both). A significant


difference in levels of caffeoylvaleroylquinic acid was not found across any pairwise comparisons. The doubly-esterified quinic acids assigned as dimethoxy-cinnamoyl derivatives were


detected at much higher levels in stenophylla than in the other two species (_P_ < 0.001 for all) (Fig. 2; Supplementary Fig. S2). OTHER ACIDS Lower levels of both quinic (3) and malic


acids (4) were observed in samples of stenophylla than in Arabica (_P_ < 0.001 for both), although no such difference was observed for citric acid (5, see Fig. 2). Comparison through TOST


found levels of citric acid in Arabica and stenophylla to be similar (_P_ = 0.018). DITERPENOIDS Of all the classes of compounds detected in the three species, the diterpenoids showed the


most interspecific variation. Only trace amounts of diterpenoids were detected in the robusta samples, contrasting with a range of diterpenoids detected in stenophylla and Arabica.


Interestingly, many of these were only detected in either one species or the other, rather than both; previous reports have described certain coffee diterpenoids as highly variable in


content and profile between species, including stenophylla50. Several atractyloside derivatives were assigned in samples of Arabica and stenophylla. Of these, compounds assigned as CATR II


(17) and ATR V (34) were observed in the Arabica samples at higher levels, compared to the trace amounts detected in robusta and stenophylla. The compounds assigned as CATR I (26), CATR III


(30), and 2-_O_-glucopyranosyl-deoxyhexopyranosyl-carboxyatractyligenin (20) were detected in both Arabica and stenophylla. A compound assigned as the furokaurane glycoside, mozambioside


(19), was detected in all samples of Arabica, but not in those of stenophylla or robusta (_P_ < 0.001 for both) (Fig. 2). A compound assigned as bengalensol (23), first isolated from the


leaves of _Coffea benghalensis_51, was detected in samples of Arabica and stenophylla, though the content was significantly higher in stenophylla (_P_ < 0.001). Other compounds assigned


as diterpenoid glycosides included a diterpenoid dihexoside (15) only detected in stenophylla, and two compounds with the molecular formula C26H42O10 (14 & 16), consistent with that of


cofaryloside or isomers52, both were only detected in samples of Arabica. OTHER COMPOUNDS The levels of sucrose (2) in Arabica and stenophylla were similar (TOST _P_ < 0.001, see Fig. 2);


in contrast, the level of sucrose in robusta was significantly lower than Arabica (_P_ = 0.0017). Compounds assigned as tyrosine and tryptophan derivatives were detected at higher levels in


robusta, compared to Arabica and stenophylla (_P_ < 0.001 for all assigned compounds); these detected compounds were assigned as _N_-caffeoyltyrosine (18), _N_-caffeoyl-, _N_-feruloyl-,


and _N_-hydroxycoumaroyl-tryptophan (27, 33, & 31, see Supplementary Fig. S2). This finding is in accordance with previous reports, in which caffeoyltyrosine and related compounds were


considered to be chemotaxonomic markers of robusta coffee53. Compounds assigned as the serotonin derivatives _N_-eicosanoylserotonin (36) and _N_-docosanoylserotonin (37) were detected in


all three species; stenophylla had significantly lower levels of the latter than Arabica (_P_ < 0.001), but no such difference was observed for _N_-eicosanoylserotonin (Fig. 2).


METABOLOMIC ANALYSES PCA of the overall metabolomes shows a clear distinction between the three species (Fig. 3), which is verified through PERMANOVA tests on the four pictured principal


components, the first 16 components which explain 80% of the variance, and all the components (_P_ < 0.001 in each case). When Logistic Regression models are trained to classify species


based on the principal components, if only PC1 is used the model achieves 88% accuracy in leave-one-out cross-validation. Using PC1, PC2, …, PCj for 1 < _j_ ≤ _N_, the model achieves 100%


accuracy—confirming that these species can be reliably delineated based on their chemical profiles. Considering the loadings generated in the PCA for each of the 37 assigned compounds (Fig.


S3), some distinct groupings are observed. This is clearest in the case of stenophylla where the samples appear to form a dense group loading negatively on PC1 and PC2, associated with high


values for compounds 7, 15, 20, 21, 32, and 35. Similarly, robusta samples are associated with high values for 6, 9, 10, 11, 13, 18, 24, 25, 27, 29, 31, and 33. For Arabica, though the plot


appears to identify two distinct groups, in general the samples are associated with positive values in PC2, related strongly to compounds 17 and 19. These findings align with the plots


presented in Fig. 2 and Supplementary Fig. S2. Hierarchical clustering heatmaps of the metabolomes of each sample analysed (Fig. 4) identify several characteristic regions of compounds for


the three species, similar to those identified in the PCA loading plot (Fig. S3). Group A shows high contents for robusta that are not present in stenophylla, or most Arabica samples,


although there is some overlap with group D. Group A also highlights some within-species variation for robusta where contents are lower outside of A, with some exceptions in group B. Group C


shows a distinctive region of high contents in stenophylla that are mostly not found in the other species, similarly for Group E and Arabica. DISCUSSION The similarity in caffeine content


between stenophylla and Arabica is highly relevant, since the stimulant and nootropic effects of caffeine are a contributing factor to the coffee experience and its market success26.


Furthermore, caffeine is linked to bitterness in coffee, and thus its characteristic flavour profile. Trigonelline, observed at similar levels in Arabica and stenophylla, may also contribute


to bitterness, although more indirectly, since trigonelline content is reduced during roasting and as a result, bitterness is linked to the formation of nicotinic acid54. Indeed, the


resultant nicotinic acid is strongly associated with bitterness in roasted coffee55. Detection of theacrine in stenophylla coffee is particularly interesting because until this study,


theacrine had not been reported in coffee beans. Theacrine was first isolated from plants as crystals in the residues left over after de-caffeinating large quantities of tea56. Since then it


has been identified as a constituent of certain varieties of tea57,58 and detected as a minor metabolite in species of _Ilex_59, _Theobroma_60, and in the leaves of certain other species of


_Coffea_, such as _C. liberica_61. Whilst the central nervous system stimulating and cognitive performance enhancing effects of caffeine are well-documented62,63, theacrine has not been


studied as extensively as caffeine, though some studies have associated theacrine with improving cognitive performance without habituation64. Other studies suggest theacrine is sedative and


hypnotic in vivo via non-selective adenosine receptor agonism65, thus contrasting with the stimulatory action of caffeine associated with adenosine A2A receptor antagonism62. Considering


that theacrine attenuated caffeine-induced insomnia in vivo65, the stimulatory effect of stenophylla coffee (which contains both caffeine and theacrine) would be of particular interest to


evaluate. The theacrine content of stenophylla brewed as a beverage is likely to vary depending on the brewing method, considering that theacrine may leach into water significantly more


slowly than caffeine, as has been observed with certain varieties of tea, thus requiring a much longer brewing time57. In “_kucha”_ tea, theacrine has been positively correlated with


bitterness66. Taste tests have shown theacrine to have a significantly lower threshold of recognition than caffeine, making it likely to contribute disproportionately more to bitterness than


caffeine, relative to its content57. The biosynthetic pathway leading to theacrine production has been elucidated in tea67,68. A similar metabolic pathway has been proposed in _C.


liberica_, which accumulates small amounts of theacrine in the leaves61, although in other _Coffea_ species, the majority of caffeine is instead catabolised to xanthine by way of


theophylline69. Considering all the hydroxycinnamic acids and derivatives together, their content in stenophylla was overall similar to Arabica, suggesting they may contribute to the


similarity in flavour between these two species. The profile of these compounds observed in robusta was different (Supplementary Fig. S2). A high level of intraspecific variation in levels


of some of these compounds was observed, which might be partially explained by differences in growing conditions, as has been previously reported in coffee70. Chlorogenic acid derivatives


have been considered as chemical drivers of coffee quality, modulating coffee flavour to significantly increase coffee cup score71. These chemicals are thought to contribute to the bitter


taste, acidity, and astringent flavour of coffee, even though levels may reduce by around 50% after roasting24. The hydroxycinnamic (chlorogenic) acids, 3- and 4-_O_-caffeoylquinic acids in


particular, have been linked to the sensation of ‘mouth-coating’ (or residual oiliness in the mouth after drinking) in coffee72. Interestingly, this has been proposed to show an inverse


correlation, so the increased levels in robusta (Fig. S2) may result in a reduced perception of mouth-coating. The overall effect of doubly-esterified quinic acid derivatives on coffee


flavour is linked to bitterness and astringency73,74. Although the content of these compounds may be reduced on roasting, many of the resultant products formed have been identified as highly


bitter, especially esterified quinic acid lactones74. As such, the higher levels of many of these compounds found in robusta than in the two other species (Fig. 1) may contribute to


differences in flavour. If the dimethoxycinnamoyl quinic acid derivatives detected in stenophylla act akin to other doubly-esterified quinic acids, their roasting products might also


contribute to bitterness74. As these compounds were not detected in Arabica or robusta, they may be potential markers to distinguish stenophylla from Arabica and robusta (Fig. 1). The


content of certain small organic acids in unroasted coffee beans has been associated with sensory attributes in roasted coffee, including sourness, acidity, fruitiness, astringency, and


bitterness75. The lower levels of quinic and malic acids observed in stenophylla (Supplementary Fig. S2) may contribute to observed differences in acidity and ‘fruity’ notes in sensory


tasting compared to some Arabica samples3,9. However, the comparable citric acid content of both Arabica and stenophylla is of particular interest for this compound’s relevance to overall


coffee flavour. Recent studies have shown that citric acid is the only small organic acid present in coffee which has a threshold of detection below the concentrations typically found in


brewed coffee23. As such, it is much more likely to be involved in sensory attributes than other acids. Certain atractyloside derivatives have previously been linked to a reduction in


apparent bitterness in brewed coffee76, therefore their detection in the coffee species analysed, and notably the detection of atractylosides common to both stenophylla and Arabica, could be


a contributing factor to explain the similarity in the flavour of Arabica and stenophylla. Detection of a compound assigned as mozambioside in Arabica, but not in robusta (Fig. 2), is


consistent with previous reports which describe only trace quantities in robusta77. Mozambioside has a bitter taste recognition threshold about ten times more potent than caffeine78, and


some of its degradation products formed on roasting are even more bitter79, so it has been considered likely to be a major contributor to the bitter taste of Arabica. That it was not


detected in stenophylla suggests that other compounds contribute to their similarity in flavour. Mozambioside may be a useful marker for Arabica coffee, since it was detected in all Arabica


samples but not in the other species analysed. The apparent lack of mozambioside in stenophylla might perhaps be partially compensated for by the presence of other bitter-tasting


diterpenoids, such as the compound assigned as bengalensol, which is associated with bitterness in coffee beans78. Sucrose, the most abundant carbohydrate in green coffee beans, has been


considered an important contributor to coffee taste24,80,81. It is also a precursor to various roasting products, including small organic acids such as formic, acetic, and lactic acids,


which can influence coffee flavour82. The similarity in sucrose content of Arabica and stenophylla is therefore likely to be an important contributor to their similarity in flavour,


particularly given the importance of caramelisation reactions during roasting80. However, the sucrose content of green coffee beans is, by itself, considered to be a poor predictor of coffee


quality26. Direct comparison of the sucrose content between species is likely to be further complicated by variations in post-harvest processing, as sucrose is amongst the compounds whose


concentration is most impacted by processing and storage conditions22. The influence of other compound classes detected in the coffee species analysed, including those assigned as


tryptophan, tyrosine, or serotonin derivatives, on coffee taste has not previously been explored but their occurrence merits further investigation in relation to coffee flavour. The PCAs


(Fig. 3) and hierarchical clustering heatmap (Fig. 4) show that it is possible to distinguish the three coffee species using metabolomic analysis, based on green bean chemistry. This could


be useful in quality control approaches, should stenophylla, or hybrids involving this species, reach the market; particularly since a niche product commanding a higher price, such as


stenophylla coffee, could be vulnerable to adulteration or mislabelling. Chemical means of detecting coffee adulteration have previously been explored, including for the identification of


non-coffee adulterants such as chicory or barley83, distinguishing Arabica and robusta coffee84, or to indicate geographic origin of coffee samples53. Robust approaches for coffee


identification combined with traceability are highly desirable in the coffee sector. To support this, our study suggests that the chemical profiles (especially mozambioside and theacrine


contents) of green beans could be potentially useful to distinguish stenophylla from Arabica and robusta. In conclusion, this is the first report to show that certain compounds considered to


influence coffee quality and flavour occur in unroasted seeds (green coffee beans) of stenophylla coffee (_C. stenophylla_). We also evaluate the potential chemical basis for the similarity


in flavour between stenophylla and Arabica coffee. We reveal that a range of compounds associated with coffee flavour can be detected in both stenophylla and Arabica, with similar levels of


caffeine, chlorogenic acids, trigonelline, sucrose, and citric acid being observed, and that their occurrence is less similar to robusta (Fig. 1, Fig. 2, Supplementary Fig. S2). These


results provide some explanation for observations that the flavour of stenophylla and Arabica coffees are similar, and that the flavour of stenophylla is Arabica-like3,9. Similarities in the


occurrence of these compounds in both stenophylla and Arabica is compelling, given the lack of relatedness (phylogenetic distance), morphological dissimilarity (e.g., black versus red


fruits, respectively), geographical separation, and environmental differences3 between these species (as elaborated in the Introduction). Despite the chemical similarities, numerous


differences between stenophylla and Arabica were also observed (Fig. 1, Fig. 2, Supplementary Fig. S2). Notably, we detected theacrine in stenophylla (and not in Arabica), which is the first


report of this alkaloid in the beans of a coffee species. The longer half-life of theacrine, combined with reports that it does not have the same stimulant effects as caffeine65,85, may


provide opportunities for the development of new coffee beverages with different properties to Arabica and robusta. Another key difference between stenophylla and Arabica is the negligible


occurrence of the compound assigned as mozambioiside in stenophylla (and robusta), compared to Arabica, suggesting this compound could be useful as a chemical marker for Arabica and


particularly to distinguish it from stenophylla and robusta. In addition, our metabolomic analyses demonstrate that the three coffee species (stenophylla, Arabica, robusta) can be reliably


distinguished and characterised based on green bean chemistry, even considering the intra-specific variation observed with Arabica and robusta. While this study highlights the similarities


in green bean chemistry between stenophylla and Arabica, we also report clear dissimilarities between the two species. Given similarities in flavour perception, yet differences in green bean


chemistry, our study may be useful for gaining a better understanding of the chemical basis of coffee flavour. It may also offer opportunities for sensory diversification and thus coffee


market differentiation, against a background of a changing climate and the need to sustain global coffee supplies for the future. DATA AVAILABILITY Data is provided within the paper or


supplementary information files. Further data are available upon request to the authors. CODE AVAILABILITY Scripts used to analyse data can be found at


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Google Scholar  Download references ACKNOWLEDGEMENTS We thank Union Hand-Roasted Coffee (UHRC) and DR Wakefield for supplying several samples of unroasted Arabica and robusta coffee beans


used in this study. This study was supported by the Amar-Franses & Foster-Jenkins Trust, the Sucafina coffee company and the Calleva Foundation. AUTHOR INFORMATION Author notes * These


authors contributed equally: Eliot Jan-Smith, Harley Downes. AUTHORS AND AFFILIATIONS * Royal Botanic Gardens Kew, Richmond, UK Eliot Jan-Smith, Harley Downes, Aaron P. Davis, Adam


Richard-Bollans, Geoffrey C. Kite & Melanie-Jayne R. Howes * Pharmaron UK Ltd, West Hill Innovation Park, Hertfordshire, UK Harley Downes * Department of Agriculture, Health and


Environment, Natural Resources Institute, University of Greenwich, Medway, UK Jeremy Haggar * Coffee Culture, Kenema, Sierra Leone Daniel Sarmu * Institute of Pharmaceutical Science, King’s


College London, London, UK Melanie-Jayne R. Howes Authors * Eliot Jan-Smith View author publications You can also search for this author inPubMed Google Scholar * Harley Downes View author


publications You can also search for this author inPubMed Google Scholar * Aaron P. Davis View author publications You can also search for this author inPubMed Google Scholar * Adam


Richard-Bollans View author publications You can also search for this author inPubMed Google Scholar * Jeremy Haggar View author publications You can also search for this author inPubMed 


Google Scholar * Daniel Sarmu View author publications You can also search for this author inPubMed Google Scholar * Geoffrey C. Kite View author publications You can also search for this


author inPubMed Google Scholar * Melanie-Jayne R. Howes View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS M.-J.R.H. and A.P.D. conceived the


study. H.D. prepared the extracts for LC–MS analysis and undertook data analyses; G.C.K. acquired the LC–MS data for analysis; E.J.-S., and M.-J.R.H. carried out supporting analyses.


E.J.-S., A.R.-B. and H.D. analysed the data, with A.R.-B. performing the statistical analyses. E.J.-S., M.-J.R.H. and A.P.D. wrote the paper. D.S., J.H and A.P.D undertook project


management, fieldwork and sample collection. All authors contributed to the critical review of the paper. CORRESPONDING AUTHOR Correspondence to Melanie-Jayne R. Howes. ETHICS DECLARATIONS


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Arabica-like flavour of stenophylla coffee and the chemistry of quality coffee. _npj Sci Food_ 9, 33 (2025). https://doi.org/10.1038/s41538-025-00398-8 Download citation * Received: 30


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