ABSTRACT The rice blast fungus _Magnaporthe oryzae_ uses a pressurized infection cell called an appressorium to drive a rigid penetration peg through the leaf cuticle. The vast internal

pressure of an appressorium is very challenging to investigate, leaving our understanding of the cellular mechanics of plant infection incomplete. Here, using fluorescence lifetime imaging

of a membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension in _M. oryzae_. We show that extreme pressure in the appressorium leads to large-scale spatial

heterogeneities in membrane mechanics, much greater than those observed in any cell type previously. By contrast, non-pathogenic melanin-deficient mutants, exhibit low spatially homogeneous

hosts, offering the potential for new disease intervention strategies. SIMILAR CONTENT BEING VIEWED BY OTHERS REVEALING REAL-TIME 3D IN VIVO PATHOGEN DYNAMICS IN PLANTS BY LABEL-FREE OPTICAL

COHERENCE TOMOGRAPHY Article Open access 27 September 2024 DISSECTING THE MEMBRANE-MICROTUBULE SENSOR IN GRAPEVINE DEFENCE Article Open access 01 December 2021 CARBOTAG: A MODULAR APPROACH

FOR LIVE AND FUNCTIONAL IMAGING OF PLANT CELL WALLS Article Open access 01 May 2025 MAIN Many plant pathogens use specialized infection cells called appressoria to infect their hosts1,2,3.

Appressoria facilitate pathogen entry into host tissue to cause disease, and the famous ‘gold leaf’ experiment demonstrated the capacity of some fungal appressoria to puncture the leaf

surface using force generation rather than enzymatic activity2,4. Appressoria of the rice blast fungus _Magnaporthe oryzae_ (synonym of _Pyricularia oryzae_)5—a major threat to global food

security6,7,8—breach the tough surface of rice leaves and, remarkably, other hard synthetic surfaces by generating turgor of up to 8.0 MPa (~40 times the pressure of a car tyre9). This

generates force at the base of the appressorium, measured in a related pathogen _Colletotrichum graminicola_ using an optical waveguide, of 17 µN (ref. 10). By contrast, pressures measured

in fungal or oomycete hyphae seldom exceed 0.8 MPa (ref. 11). _M. oryzae_ appressoria have a melanin-lined cell wall impermeable to glycerol, but freely permeable to water, which rapidly

enters the cell, generating hydrostatic pressure. Mutation of the melanin biosynthetic enzyme-encoding genes _ALB1, RSY1_ and _BUF1_ causes loss of appressorium melanization, which enables

movement of solutes and water through the appressorium cell wall, leading to loss of turgor generation and the ability to cause disease12,13. Directly measuring appressorium turgor has been

challenging because of the enormous pressure generated, which preclude the use of pressure probes. Instead, researchers have used indirect methods such as the incipient cytorrhysis assay,

which records the rate of cell collapse when appressoria are incubated in hyperosmotic solutions9,14,15. However, melanin-deficient mutants undergo plasmolysis rather than cell collapse when

exposed to high concentrations of glycerol limiting the use of this assay9,14. The Flipper-TR probe, which contains a membrane-targeted twisted push–pull fluorophore is sensitive to

mechanical forces acting on the plasma membrane. Previous reports have suggested the fluorescence lifetime of the probe changes linearly with plasma membrane tension in both yeast and

mammalian cells16. In _M. oryzae_, the probe has been used to measure plasma membrane tension in hyphae of Guy11 and a _∆vast1_ mutant, which affects TOR17 signalling, and is implicated in

the cAMP response, cell integrity and control of autophagy18,19,20,21,22. But, while the probe suggests that the _∆vast1_ mutant has increased tension, experiments were performed only in

hyphae23. Here we set out to explore whether we could visualize turgor generation in appressoria directly. Recently, a set of chemically modified molecular rotors were developed to yield

complete microviscosity maps of cells and tissues in the cytosol, vacuole, plasma membrane and wall of plant cells24. These boron-dipyrromethene-based molecular rotors are rigidochromic,

meaning their fluorescence lifetime depends on the mechanics of their surroundings, such as viscosity or membrane tension. The N+-BDP plasma membrane probe has, for instance, revealed

differences in membrane mechanics between the plant root cap and the meristem. Fluorescence lifetime imaging microscopy (FLIM) revealed the plant meristem to undergo continuous growth and

cell division, resulting in constant tension in the plasma membrane24. The tension increases spacing between lipids, leading to significant reduction in membrane rotor lifetime compared to

the relaxed plasma membranes of root cap cells25. Furthermore, closer examination of the plasma membrane revealed distinct lipid microdomains within a single bilayer. Likewise, in root hairs

the fluorescence lifetime was lower at the growing tip (3.6 ± 0.8 ns), compared with the non-growing epidermal cell plasma membrane (4.3 ± 0.6 ns). The change in lifetime corresponds to the

increase in tension in the growing root hair tip, where membrane curvature is greatest. Plasmolysis assays in rotor-stained root hairs confirmed the probe’s responsiveness to changes in

tension within _Arabidopsis_ root tissues, as the fluorescence lifetime within root hair tips significantly increased upon exposure to hyperosmotic stress and a drop in membrane tension. In

this Article, we report that the mechanosensor N+-BDP plasma membrane rotor probe can detect spatial variations in membrane tension in _M. oryzae_ appressoria. We reveal how changes in

microviscosity correlate with appressorium-mediated turgor-driven plant cell infection and show that, under the extreme pressures of an appressorium, the plasma membrane exhibits spatial

heterogeneity in tension, a phenomenon not previously observed in living cells. We provide experimental validation of the requirement of melanin biosynthesis for appressorium turgor

generation in _M. oryzae_ and show that the Sln1 turgor sensor kinase26,27 is necessary for controlling both the rate of turgor generation and its modulation before plant infection. The

N+-BDP mechanosensor therefore provides a direct, quantitative measurement of the average tension at the appressorium membrane enabling new insight into the behaviour of eukaryotic cells

under extreme pressure and the mechanobiology of plant infection by the blast fungus. RESULTS N+-BDP REVEALS SPATIAL VARIATIONS IN PLASMA MEMBRANE TENSION IN _M. ORYZAE_ We first determined

whether the mechanoprobe N+-BDP could reveal changes in appressorium-specific membrane tension during a time course of infection-related development of the wild-type _M. oryzae_ strain

Guy11. During initial stages of appressorium development, 4 h after conidia have germinated on hydrophobic glass coverslips, incipient appressoria are not melanized and have not yet

generated turgor. By contrast, 24 h after inoculation, appressoria are mature and fully melanized, and generate high levels of turgor, deployed as mechanical force as appressoria are tightly

bound to the hydrophobic surface, creating a tight seal necessary for appressorium function8,28,29 (Fig. 1a). The chemical structure of the mechanoprobe N+-BDP is based on a modified

phenyl-substituted boron-dipyrromethene molecular rotor, in which the phenyl ring carries an aliphatic tail with two permanent cationic charges, creating a positive charge and thereby

targeting the negatively charged phospholipid bilayer24 (Fig. 1b). Upon staining, the probe is positioned between the tails of the bilayer, with its aliphatic tail facing towards

phospholipid heads (Fig. 1c). Previous work using rotor-stained giant unilamellar vesicles (GUVs) composed of sphingomyelin, 1,2-dioleoyl-_sn_-glycero-3-phosphocholine and cholesterol

(0.56:0.24:0.20) has enabled study of lipid phase transition. The lipid phase separation in GUVs creates an inhomogeneous biological membrane composed of different lipid microdomains,

similar to formation of lipid microdomains in biological membranes by immiscibility of different lipids24,30,31,32. Upon staining different GUVs, the N+-BDP mechanoprobe demonstrated a

stronger mechanical restriction for rotations imposed by the tightly packed and solid-like sphingomyelin-rich gel-like ordered phase, generating longer average fluorescence lifetimes

compared with the less tightly packed liquid-like phase enriched in 1,2-dioleoyl-_sn_-glycero-3-phosphocholine. When considering an appressorium, we hypothesized that early-stage (4 h)

appressoria would display a more compact membrane as a result of being under little or no tension, thereby causing mechanical restriction of the rotor probe upon photoexcitation and longer

average fluorescence lifetimes (Fig. 1c). In a 24 h appressorium meanwhile exhibiting high appressorium turgor and high membrane tension, the membrane would become stretched and disordered,

allowing free rotation of the probe and consequently shorter average fluorescence lifetimes (Fig. 1d). To test this hypothesis, we used the N+-BDP mechanoprobe to stain 4 h and 24 h

appressoria of _M. oryzae_ Guy11 to observe the spatial variations in membrane tension (Fig. 1e). Strikingly, when we selected the appressorium membrane for analysis (Extended Data Fig. 1)

we observed that 4 h appressoria displayed a homogeneous high average rotor lifetime of 3.98 ± 0.084 ns (Fig. 1f and Supplementary Video 1), in contrast to mature appressoria (7.5–24 h)

which displayed spatial heterogeneity and a significantly lower average rotor lifetime of 2.79 ± 0.026 ns (Fig. 1f and Supplementary Video 2). Intriguingly, in contrast to the low

fluorescence lifetimes we consistently observed in appressoria, we observed consistent and uniform high fluorescence lifetimes in _M. oryzae_ germ tubes (Extended Data Fig. 2). Considering

the primary function of the germ tube is to deliver the contents of the conidium to the developing appressorium for maturation, these cells have no requirement for extreme turgor generation,

which is corroborated by the rotor probe. We next tested whether artificially lowering the turgor of appressoria by incubation in hyperosmotic concentrations of glycerol, would

independently corroborate probe responsiveness to changes in membrane tension within an appressorium. Under hyperosmotic conditions, the fluorescence lifetime in a 24 h appressorium

significantly increased from 2.79 ± 0.041 ns to 3.10 ± 0.067 ns upon addition of exogenous 1 M glycerol (Extended Data Fig. 3). This change is consistent with a drop in tension as water

exits the appressorium by osmosis13,26. Considering melanin biosynthesis and deposition occur between 4 h and 8 h post-inoculation (hpi) on a glass coverslip8, we reasoned that this would

provide a suitable time to capture changes in local membrane tension and turgor. A real-time movie of a Guy11 appressorium stained with the N+-BDP rotor probe was captured during a 3 h

period, in which spatial variations in membrane tension were apparent and the overall fluorescence lifetime decreased (Fig. 1g, Supplementary Video 3 and Extended Data Fig. 4). Our initial

analyses of turgor generation were carried out on unyielding glass coverslips, so we were interested in observing appressorium turgor on surfaces that can be penetrated. As the N+-BDP

mechanoprobe stains all membranes it encounters, imaging appressoria on a leaf surface is technically challenging. We therefore inoculated sterile onion epidermis, pre-treated with

chloroform before washing in sterile water (Extended Data Fig. 5a), which can be readily penetrated by the fungus. We found that appressorial membranes displayed consistent spatial

heterogeneity (Extended Data Fig. 5b) and a very similar lifetime compared with those on an unyielding coverslip surface at 2.69 ± 0.083 ns (Extended Data Fig. 5c). We conclude that the

dynamics of appressorium turgor generation are similar on both yielding and unyielding surfaces. To determine whether the N+-BDP mechanoprobe can be applied to other appressorium-forming

fungal pathogens, we tested appressoria from the hemibiotrophic ascomycete fungus _Colletotrichum higginsianum_, which causes anthracnose disease on _Arabidopsis_33. During the initial

stages of appressorium development, once conidia have germinated on hydrophobic glass coverslips, incipient appressoria of _C. higginsianum_ are not melanized and have not yet generated

turgor. By contrast, 24 h after inoculation, appressoria are mature and fully melanized (Fig. 2a). We used the N+-BDP mechanoprobe to stain early-stage (non-melanized) (Fig. 2b) and

late-stage (melanized) (Fig. 2c) appressoria of _C. higginsianum_, to observe spatial variations in membrane tension. Early-stage appressoria displayed homogeneous high average rotor

lifetime of 4.301 ± 0.043 ns (Fig. 2d), in contrast to late-stage appressoria which displayed a significantly lower average rotor lifetime of 3.72 ± 0.085 ns, consistent with our findings

for _M. oryzae_. We conclude that the N+-BDP mechanoprobe can be widely deployed for generating membrane tension maps in appressorium-forming pathogens. To confirm that the surprising large

inhomogeneties observed in _M. oryzae_ 24 h appressoria are due to changes in membrane tension and not the result of formation of compositional membrane microdomains or dye interaction with

the hydrophobic melanin layer, we performed control experiments using a plasma membrane sensor NR12S, a solvatochromic Nile red-based probe34. In the same way as the N+-BDP probe, the Nile

red chromophore is functionalized with a lipid-like alkyl tail for membrane targeting (Extended Data Fig. 6a). This probe, however, is not sensitive to membrane tension, but rather exhibits

a shift in emission wavelength in response to changes in local chemical polarity, such as lipid and sterol composition of the membrane. Ratiometric imaging, in which the total emission of

the dye is split into two channels, provides a non-lifetime-based readout for the probe. Changes in membrane chemical composition and lipid phase affect the chemical polarity of the probe

microenvironment, initiating a change in the intensity ratio between the blue and red channels35 (Extended Data Fig. 6b,c). Previously, this probe was used for mapping spatial variation in

plasma membrane chemical polarity of _Phytophthora infestans_ germlings35. We observed that chemical polarity of appressoria becomes globally lower during appressorium maturation. In early 4

 h appressoria, ratiometric imaging of the appressoria plasma membrane appears blue, indicating the membrane has a high chemical polarity that is is less ordered. (Extended Data Fig. 6d–j).

However, in 24 h appressoria, the plasma membrane appears yellow and red, indicating the membrane has a low polarity indicative of a higher lipid packing order. (Extended Data Fig. 6k–p).

Importantly, the magnified sections of the plasma membrane for both 4 h appressoria (Extended Data Fig. 6e,f,h,i) and 24 h appressoria (Extended Data Fig. 6l,m,o,p) shows the probe NR12S

displaying either homogeneous polarity, or polarity variations whose pattern is not consistent with the larger changes in tension we observe with the 4 h and 24 h appressoria stained with

the N+-BDP rotor (for comparison, see Extended Data Fig. 6). We conclude that N+-BDP reveals spatial changes in appressorium-specific tension during infection-related development of _M.

oryzae_ and that the observed patterns are not caused by concurrent inhomogeneities in membrane composition. MELANIN IS CRITICAL FOR _M. ORYZAE_ APPRESSORIUM TURGOR The synthesis of

dihydroxy-naphthalene (DHN) melanin in _M. oryzae_ has long been reported to be essential for appressorium-specific turgor-driven plant infection7,13. A layer of melanin is located between

the appressorium membrane and cell wall where it acts as a structural barrier to efflux of solutes from the appressorium, essential for turgor generation and pathogenicity7,13. Mutation of

genes encoding enzymes required for DHN-melanin biosynthesis, _ALB1_, _RSY1_ and _BUF1_, causes impairment in appressorium and hyphal melanization12. Consequently, melanin-deficient mutants

fail to infect intact host plants12. We determined whether N+-BDP could detect a reduction in membrane tension in _alb1__-_ and _buf1__-_ mutants compared with the isogenic wild-type Guy11.

Both _alb1__-_ (Supplementary Video 4) and _buf1__-_ mutants displayed homogeneous high fluorescence lifetimes of 3.23 ± 0.063 ns and 3.20 ± 0.056 ns, respectively, similar to values for

non-melanized 4 h appressoria in Guy11 (Fig. 3a,b,e). Furthermore, when Guy11 was exposed to the melanin biosynthesis inhibitor tricyclazole at 3 h compared to an untreated control, we

observed a high fluorescence lifetime of 3.18 ± 0.031 ns, consistent with lifetimes of the melanin-deficient mutants and low appressorium tension (Fig. 3c–e). The mechanoprobe N+-BDP

therefore demonstrates that _alb1__-_ and _buf1__-_ mutants do not generate appressorium turgor, and furthermore, that tension in the appressorial membrane of melanin mutants and

tricyclazole-treated Guy11 is universally low. We also tested whether N+-BDP could detect an increase in membrane tension in non-melanized germ tube tips (Fig. 4a) during polarized growth

compared with the wild type (Fig. 4b). Interestingly, the tips and points of curvature of germ tubes exhibited a lower fluorescence lifetime of 3.84 ± 0.078 ns compared with the subapical

walls of the germ tube that displayed an average higher lifetime of 4.20 ± 0.0773 ns (Fig. 4b). Considering that melanin deposition has not begun at 3 hpi, with enzymes involved in melanin

biosynthesis peaking in expression between 6 hpi and 8 hpi (_ALB1_ MGG_07219, _RSY1_ MGG_05059 and _BUF1_ MGG_02252) (ref. 36) (Fig. 4c), this experiment effectively decouples melanization

from membrane tension, showing that turgor generation in actively growing non-melanized hyphal tips can be revealed by the mechanosensor. INVESTIGATING THE GENETIC CONTROL OF APPRESSORIUM

TURGOR GENERATION WITH N+-BDP To test whether N+-BDP could provide new insight into turgor control in _M. oryzae_, we tested mutants impaired in appressorium function in which the effect on

turgor generation is not known. Septins are required for pathogenicity of _M. oryzae_, regulating F-actin organization in the appressorium, and acting as lateral diffusion barriers for

proteins involved in penetration peg emergence and elongation37. A total of six septins are present in _M. oryzae_, four of which share similarity to core septins identified in yeast, Sep3,

Sep4, Sep5 and Sep6. Recently, very long chain fatty acid biosynthesis has been shown to regulate phosphatidylinositol-phosphate-mediated septin ring formation by recruiting septins to

curved plasma membranes, initiating septin ring formation and subsequent penetration peg emergence38. Septin 5 is a core septin of _M. oryzae_ that forms part of the heteropolymeric septin

ring in the appressorium pore and is essential for plant infection37. We stained a _∆sep5_ mutant with N+-BDP to see whether turgor generation is affected by absence of the septin. This

revealed no significant change in membrane tension and appressorium turgor (2.90 ± 0.059 ns) compared with Guy11 (2.79 ± 0.046 ns) (Fig. 5a,b,d), providing evidence that absence of Sep5 has

no effect on appressorium turgor generation, but instead impairs re-polarization, separating the two processes and demonstrating more clearly the precise function of septins in _M.

oryzae_37. We were also curious to test the _∆nox2_ mutant, because previous work has shown that in the absence of _NOX2_, septins and F-actin do not assemble at the appressorium pore39. In

addition to playing an important role in septin-mediated cytoskeletal re-organization, Nox enzymes are implicated in the chemiosmotic generation of turgor pressure, particularly in mammalian

cells40. Staining the _∆nox2_ mutant with the N+-BDP rotor probe revealed a significant reduction in membrane tension (3.12 ± 0.041 ns) compared with the wild-type Guy11 (2.79 ± 0.046 ns)

(Fig. 5a,c,d), suggesting that absence of the Nox2 NADPH oxidase catalytic subunit does affect turgor generation in the appressorium. We conclude that the mechanoprobe N+-BDP is effective as

a means of screening mutants impaired in appressorium function for a role in turgor pressure generation. THE SLN1 KINASE CONTROLS TURGOR THROUGHOUT APPRESSORIUM DEVELOPMENT The Sln1

histidine-aspartate kinase in _M. oryzae_ acts as a sensor to detect when a critical threshold of turgor has been reached in the appressorium to enable host penetration26. As a consequence

Δ_sln1_ mutants are unable to sense turgor and their appressoria are predicted to have excess turgor pressure and are hyper-melanized26,27. We therefore tested whether the N+-BDP

mechanoprobe could detect aberrant turgor generation in a Δ_sln1_ mutant. First, we used septin–green fluorescent protein (GFP) localization to determine the time at which maximum turgor is

achieved, when a septin ring is formed in the appressorium pore to facilitate re-polarization (Fig. 6a and Supplementary Video 5)8,37,41. F-actin and septin ring recruitment occurs in a

pressure-dependent-manner26,42 while in a melanin-deficient _buf1__-_ mutant12, septin and F-actin localization are disordered26,41,42. The hyper-melanized Δ_sln1_ mutant also displays

aberrant septin and actin localization patterns (Fig. 6c and Supplementary Video 6)26. To investigate whether the N+-BDP could detect the predicted abnormal turgor of Δ_sln1_ mutants we

carried out staining of a time course of infection-related development and determined the average fluorescence lifetime at each developmental stage. In Guy11 appressoria at 4 hpi, an average

lifetime of 3.95 ± 0.091 ns was observed, which significantly reduced to 3.11 ± 0.061 ns at 6 hpi, consistent with initiation of melanin synthesis and onset of turgor generation. By 8 hpi

the average lifetime had significantly reduced again to 2.81 ± 0.079 ns, and by 24 hpi the average lifetime was 2.73 ± 0.042 ns. The average lifetime of N+-BDP fluorescence did not

significantly change between 8 hpi and 24 hpi, suggesting either that membrane tension remains constant after 8 hpi or that the rotor probe is saturated and unable to resolve higher tensions

(Fig. 6b,e). The commitment point for septin ring organization is between 8 h and 10 h (refs. 8,37). Previously, septin ring formation was shown to be impaired after lowering appressorium

turgor by application of exogenous glycerol, or treatment with tricyclazole when applied up to 16 hpi (ref. 26). This suggests that appressorium turgor reaches a critical threshold before

septin ring assembly, and its modulation and maintenance through action of the Sln1-turgor-sensing-complex helps to stabilize conditions required for preserving septin ring organization. In

the Δ_sln1_ mutant we observed significantly higher membrane tension particularly between 4 hpi and 6 hpi, averaging 3.70 ± 0.057 ns and 2.89 ± 0.110 ns, respectively. By 8 hpi the average

lifetime significantly reduced to 2.55 ± 0.057 ns, and by 24 hpi the average lifetime observed was 2.51 ± 0.062 ns. Once again, the lifetime of the N+-BDP rotor probe remained constant

between 8 hpi and 24 hpi, but the lifetime was significantly lower at 4 hpi, 8 hpi and 24 hpi compared with Guy11 (Fig. 6d,e and Supplementary Video 7). N+-BDP therefore reveals that Sln1 is

necessary for controlling the temporal dynamics of turgor generation throughout appressorium development, because the Δ_sln1_ mutant exhibits excess turgor even at 4 hpi. In the current

model for the action of Sln1, it is proposed to act as a negative regulator of turgor once a threshold of turgor has been achieved. The model is therefore likely to be incorrect, or at least

incomplete because it appears likely that the Sln1 kinase orchestrates turgor generation throughout appressorium development, controlling both the rate of turgor build-up and when

sufficient pressure has been generated to facilitate the switch to polarized growth. Live cell imaging with the N+-BDP rotor probe therefore reveals new insight into the regulation of

appressorium turgor generation. DISCUSSION Many fungal pathogens develop infection cells to breach the tough external barrier of a plant or animal1,2. These cells include appressoria,

hyphopodia and infection cushions2,3,28,43,44,45. Appressoria are, however, the most studied infection structure and essential for many of the most destructive plant diseases46. Economically

important pathogens such as the powdery mildew pathogen _Blumeria graminis_, the corn smut fungus _Ustilago maydis_ and soybean rust fungus _Phakopsora pachyrhizi_, for example, all

elaborate appressoria. Oomycete pathogens, such as _Phytophthora_ and _Pythium_ species also develop functional appressoria2, and recently it was shown that the late blight pathogen

_Phytophthora infestans_ enters its host at a diagonal angle, using a specific ‘naifu’ cutting action to break the host leaf surface47. The devastating rice blast fungus _M. oryzae_ uses

appressoria to breach the tough rice leaf cuticle, by generating enormous turgor of up to 8.0 MPa (ref. 13). In this study we applied a plasma membrane targeting rigidochromic molecular

rotor to generate complete mechanical maps of wild-type and mutant appressoria of _M. oryzae_. These have revealed how the mechanics of the plasma membrane are adaptively modulated to

accommodate appressorium growth and turgor generation. Creating detailed tension maps of appressoria during different stages of infection-related development has allowed us to observe

real-time changes in turgor generation. Previous studies have suggested that changes in mechanical tension of a composite lipid membrane are facilitated through formation of bulges and

protrusions of membrane domains48. In addition, other studies have suggested how mechanical stress on a membrane can increase the line tension between a microdomain and the rest of the lipid

bilayer49, which can in turn lead to microdomain growth50. How membranes deal with such extreme tensions, however, is unknown, because all membrane studies have been performed on cells with

much lower internal pressures. By contrast, our study used fungal appressoria that generate pressures that are an order of magnitude higher than any previously analysed. This revealed that

the appressorium membrane develops different microdomains when subject to extreme levels of mechanical stress. Control experiments with a probe sensitive to chemical polarity of membranes

that can reveal compositional inhomogeneities, but that is tension insensitive, showed that the unexpected tension inhomogeneities do not correlate with formation of lipid microdomains or

melanin binding of the probe. This is further corroborated by the fact that N+-BDP can detect turgor changes in the tips of non-melanized hyphae. Our study provides new information regarding

how membrane tension is distributed under extreme turgor in eukaryotic cells, revealing much larger membrane spatial heterogeneities than previously reported. While previous studies in

unwalled cells and free-standing in vitro membranes have linked tension to changes in membrane homogeneity48,50, these are not pressurized to the same extent as membranes enclosed in

appressoria. The findings presented here are therefore the first report of membrane heterogeneity under enormous pressures, and the results highlight how increases in tension can give rise

to regimes in membrane physics, which are unknown and probably reflect the extreme mechanics of an appressorium. One potential explanation for the large tension inhomogeneities is that

tension on the plasma membrane is very sensitive to the state of its anchoring to the cell wall in which it is enclosed, suggesting processes that couple wall and membrane mechanics. There

are numerous proteins, including a variety of receptors, for example, that feature both transmembrane and extracellular domains and that mechanically link the membrane to the cell wall,

potentially in a non-homogeneous manner that may be reflected in the tension patterns revealed. To test the efficacy of the N+-BDP mechanosensor, we first validated the well-known role of

melanin in appressorium turgor generation. Here the rotor dye was able to reveal the severe impairment in appressorium turgor generation and the lack of membrane heterogeneity that

accompanied reduced pressure of _M. oryzae_ melanin-deficient mutants. We also showed that mutants in which turgor dynamics have not been investigated can be readily studied using N+-BDP.

While septin assembly is necessary for appressorium re-polarization and penetration peg emergence, our analysis revealed that a Δ_sep5_ mutant did not show a significant reduction in turgor,

based on FLIM analysis. This is consistent with previous reports that have shown that septin assembly is turgor dependent and occurs only once a threshold of pressure has been generated in

the appressorium26,41. By contrast, the Δ_nox2_ mutant showed a reduction in appressorium turgor. The Nox2 NAPDH oxidase catalytic subunit is necessary for appressorium function, including

septin assembly and penetration peg formation39. Our analysis suggests that Nox2 may act upstream of septin assembly, serving a wider role in appressorium maturation than hitherto

appreciated, including ensuring that sufficient turgor is generated. To investigate whether this is a direct result of inhibiting reactive oxygen species generation, chemical inhibition with

ascorbic acid or the flavocytochrome inhibitor diphenylene iodonium could be carried out. It would also be valuable to investigate the function of the regulatory subunit NoxR in

conditioning the ability of Nox2 to regulate appressorium turgor. Finally, we tested whether N+-BDP could reveal perturbations in appressorium turgor associated with the ∆_sln1_ mutant26.

Sln1 has been proposed to act as a turgor sensor kinase and is necessary to enable a mature appressorium to re-polarize and cause infection26. A mathematical model of appressorium-mediated

plant infection predicted that a mutant lacking such a sensor would be unable to modulate appressorium turgor and therefore display excess pressure, but would be unable to re-polarize26. The

∆_sln1_ mutant displays these phenotypes, but until now its excess turgor was only predicted using the incipient cytorrhysis assay. Here we observed that ∆_sln1_ mutants do show excess

appressorium membrane tension revealed by the N+-BDP rotor. Even though we are clearly operating close to the limit of resolution of the rotor dye based on our calibration curve, because of

the enormous pressures being measured, which are well beyond the scope of pressure probes for instance, a significant difference in turgor can be determined in mature appressoria of ∆_sln1_

mutants compared with Guy11. This provides direct evidence that Sln1 does act as a sensor of appressorium turgor as predicted26, but also shows that the kinase must act earlier than

anticipated because it clearly modulates turgor during the initial stages of appressorium formation. Sln1 is therefore likely to control the rate of turgor generation, in addition to the

switch to polarized growth and septin-dependent plant infection, playing a key role in the orchestration of plant infection. When considered together, these experiments highlight the utility

of the N+-BDP mechanosensor as a direct means of analysing membrane tension in a living appressorium. Fungal mechanobiology is a new and exciting approach for studying the mechanics of the

plasma membrane, with scope to explore other compartments including the fungal cell wall, vacuoles and cytosol24. Future experiments employing the use of the molecular rotor may provide new

quantifiable insights to spatial variations in microviscosity at the point of penetration, and at crossing points during cell-to-cell movement, where it is possible that transpressoria–

which form specifically at cell junctions –generate turgor to successfully breach neighbouring cells3,51. Furthermore, a combination of surface-deformation imaging, rotor probe staining and

mathematical modelling could help to establish the precise mode of entry and the forces translated at the host leaf surface which will prove invaluable in the search for effective blast

disease control strategies47. METHODS FUNGAL STRAINS AND GROWTH CONDITIONS Growth and maintenance of _M. oryzae_ was performed as described previously52. All strains used in the study are

stored in the laboratory of N.J.T. and are freely available upon request. TWO-DIMENSIONAL FLIM IMAGING Appressorium development was induced in vitro on borosilicate 18 × 22 mm glass

coverslips (Thermo Fisher Scientific), as described previously53. For onion epidermis experiments, epidermal layers were incubated in chloroform for 30 min, washed five times in sterile

dH2O, before mounting onto a coverslip for inoculation. A 50 μl aliquot of conidial suspension (5 × 104 ml−1) was placed on a coverslip/onion epidermis and incubated at 24 °C. The aqueous

phase of the droplet from Guy11 2–3 h germ tubes, 4 h, 24 h and _C. higginsianum_ early and late appressoria was replaced with a 50 μl droplet of 10 μmol l−1 N+-BDP probe dissolved in

sterile distilled H2O. Staining was performed at room temperature for 20 min for the hyper-melanized Δ_sln1_ mutant and for 5 min for the wild-type Guy11 and all other mutants, after which

unbound dye was removed by replacing 50 μl of the droplet five times with water. For calibration of the probe in appressoria, the aqueous phase of the droplet was removed and replaced with a

50 μl droplet of glycerol (0.2 M, 0.4 M, 0.75 M or 1 M). FLIM imaging was performed on a Leica TCS SP8X upright scanning confocal microscope coupled to a PicoHarp 300 TCSPC module

(PicoQuant GmbH). Samples were excited with 488 nm output of a pulsed SuperK EXTREME supercontinuum white light laser (NKT Photonics) working at a repetition rate of 20 MHz. The full width

at half maximum of the laser pulse was ~170 ps, as determined from instrument response functions recorded using Erythrosin B (Sigma-Aldrich, >95% purity) in KI-saturated (Sigma-Aldrich)

water. Fluorescence emission was captured in the 510–530 nm range using a Leica HyD SMD detector. The objective lens was an HC Plan Apo 63×/numerical aperture 1.20 water immersion objective

(Leica Microsystems). SymPhoTime 64 (version 2.4, PicoQuant GmbH) was used to draw by hand in the 2D image a region of interest (ROI) that closely followed the membrane of the appressorium

and did not include signal arising from any other part of the sample54. The same software was used to fit the overall fluorescence decay curve of the ROI with a three-component exponential

decay function. Fits were deemed acceptable only if residuals were evenly distributed around zero and the _χ_2 values were within 0.70–1.30 range. The average fluorescence lifetimes reported

in this work are the intensity-weighted average lifetimes, which have been calculated as $$< \tau > =\frac{\sum _{i}{\alpha }_{i}{\tau }_{i}^{2}}{\sum _{i}{\alpha }_{i}{\tau }_{i}}$$

where _a__i_ and _t__i_ are the amplitude and the lifetime of species _i_, respectively55. Images are reported in a false-colour scale that represents the weighted average of fluorescence

decay for each pixel. For multi-exponential decays, the weighted average of fluorescence decay is equivalent to the intensity-weighted average fluorescence lifetime. Each datapoint in our

analysis corresponds to the weighted average fluorescence lifetime obtained for the ROI of a single 2D image of an individual appressorium. FLIM TIME SERIES EXPERIMENTS Conidia were

collected and inoculated onto glass coverslips. Early appressoria at 4 hpi were stained, washed and mounted onto glass slides, as previously described. FLIM imaging was performed on a

Stellaris 8 Falcon upright scanning confocal microscope (Leica Microsystems). Samples were excited at 488 nm using a pulsed SuperK Fianium FIB-12 PP laser source (NK Photonics) working at a

20 MHz repetition rate. The full width at half maximum of the laser pulse was ~190 ps, as determined from instrument response functions obtained using Erythrosin B solution. Detection range

and objective lens were the same as described above. A Leica HyD X detector was used and images acquired at 0 (4.5 h appressoria), 13, 31, 48, 65, 83, 109, 125, 138 and 146 min (7.5 h

appressoria). The images were processed and analysed in LAS X (version 4.2, Leica Microsystems) and movies generated using Python distributed under the GNU public licence (Anaconda Ver.

3.8.10., https://anaconda.com). Python libraries used to generate the movie were NumPy56, Scikit-image57, pystackreg58 and OpenCV59. The Python script can be found at

https://github.com/SergioGabrielLopez/movie_script. The fluorescence lifetime for each frame was obtained by selecting an ROI and then fitting the overall fluorescence decay of the ROI with

a four-exponential decay function. The fit was judged according to the previously mentioned criteria. THREE-DIMENSIONAL LIFETIME IMAGING _M. oryzae_ conidia were inoculated onto glass

coverslips, stained with N+-BDP and imaged at desired times. Images were acquired on a Stellaris 8 FALCON upright scanning confocal microscope (Leica Microsystems). All imaging parameters

were identical to those described for acquisition of the FLIM time series. The _z_-stacks had a length in the _z_-direction of ~12–15 mm, took 3–7 min to complete and were acquired in

compliance with the Nyquist-Shannon sampling theorem. The 3D rendering of the _z_-stacks was carried out in LAS X (version 4.2, Leica Microsystems). 2D IMAGING OF APPRESSORIA USING NR12S

CHEMICAL POLARITY PROBE To image plasma membrane polarity in _M. oryzae_ appressoria using the chemical polarity probe NR12S, a portion of the aqueous phase of the droplet (50 μl), was

replaced with a solution of NR12S, dissolved at 10 μmol l−1 in water. Staining was performed for 7 min, after which any unbound dye was removed by replacing 50 μl of the droplet five times

with water. Two-dimensional ratiometric imaging with NR12S was performed on a Leica TCS SP8X upright scanning confocal microscope. Samples were excited with 514 nm output of a SuperK EXTREME

supercontinuum white light laser (NKT Photonics) working at repetition rate of 80 MHz. Fluorescence was detected at 529–585 (‘blue channel’) and 610–700 nm (‘red channel’) using Leica HyD

SMD detectors. Ratiometric images obtained with NR12S staining were constructed from recorded intensity images using a custom MATLAB routine that divides photon count in each pixel of the

blue channel image, by photon count in the corresponding pixel in the red channel image. Resulting images are reported in a false-colour scale that represents the intensity ratio for each

pixel. REPORTING SUMMARY Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article. DATA AVAILABILITY The _M. oryzae_ genome

database used in this study was http://fungi.ensembl.org/Magnaporthe_oryzae/Info/Index. All _M. oryzae_ strains used in this study are freely available upon request from the corresponding

authors. Detailed protocols for synthesis of both the N+-BDP rotor probe and the chemical polarity probe NR125 are available on request from J.S. Source data are provided with this paper.

CODE AVAILABILITY The Python script used to produce the _M. oryzae_ FLIM movie has been publicly deposited in GitHub at https://github.com/SergioGabrielLopez/movie_script. REFERENCES *

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BB/V016342/1 and by the Gatsby Charitable Foundation. We thank C. Faulkner for providing us with _C. higginsianum_ strain IMI 349061. We thank C. Wood for critical reading of the manuscript.

AUTHOR INFORMATION Author notes * These authors contributed equally: Sergio G. Lopez, Lucile Michels. AUTHORS AND AFFILIATIONS * The Sainsbury Laboratory, University of East Anglia, Norwich

Research Park, Norwich, UK Lauren S. Ryder, Alice B. Eseola, Weibin Ma & Nicholas J. Talbot * Cell and Developmental Biology, The John Innes Centre, Norwich Research Park, Norwich, UK

Sergio G. Lopez * Laboratory of Biochemistry, Wageningen University & Research, Wageningen, the Netherlands Lucile Michels & Joris Sprakel Authors * Lauren S. Ryder View author

publications You can also search for this author inPubMed Google Scholar * Sergio G. Lopez View author publications You can also search for this author inPubMed Google Scholar * Lucile

Michels View author publications You can also search for this author inPubMed Google Scholar * Alice B. Eseola View author publications You can also search for this author inPubMed Google

Scholar * Joris Sprakel View author publications You can also search for this author inPubMed Google Scholar * Weibin Ma View author publications You can also search for this author inPubMed

 Google Scholar * Nicholas J. Talbot View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS L.S.R, N.J.T. and J.S. conceptualized the project.

Experimental analyses were carried out by L.S.R, S.G.L, L.M, A.B.E and W.M. The paper was written by L.S.R, J.S and N.J.T. CORRESPONDING AUTHOR Correspondence to Nicholas J. Talbot. ETHICS

DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. PEER REVIEW PEER REVIEW INFORMATION _Nature Microbiology_ thanks Ashley Garrill, Bart Thomma and the other,

anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available. ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature remains neutral

with regard to jurisdictional claims in published maps and institutional affiliations. EXTENDED DATA EXTENDED DATA FIG. 1 REGION OF INTEREST SELECTION IN TWO DIMENSIONAL FLIM IMAGES. A,

Representative FLIM image of a wild type Guy11 appressorium stained with the N+-BDP probe at 24 h. B, Image a showing the region of interest in colour with all other pixels in grayscale.

EXTENDED DATA FIG. 2 SPATIAL VARIATIONS IN MEMBRANE TENSION IN GERM TUBES AND APPRESSORIA OF _M. ORYZAE_. Representative FLIM images of wild type Guy11 24 h rotor stained appressoria and

germ tubes. Images are representative of _n_ = _3_ independent repeats of the experiment. Scale bar= 10 μm. EXTENDED DATA FIG. 3 ROTOR DYE N+-BDP CALIBRATION IN _M. ORYZAE_ APPRESSORIA

INCUBATED IN GLYCEROL. Line graph showing the average fluorescence lifetime of N+-BDP stained appressoria of Guy11 incubated in different molar concentrations of glycerol. Values are means ±

 SEM for 3 biological replicates of the experiment, total observations _n_ = 129 appressoria in 3 biological replicates; typical range 22–34 appressoria; data are presented as mean ± SEM.

***_P_ < 0.0002 as determined by one-way analysis (ANOVA) with Dunnett’s multiple comparisons test. Source data EXTENDED DATA FIG. 4 MEMBRANE TENSION OF THE APPRESSORIUM DECREASES DURING

MATURATION. Guy11 appressoria were stained with the rotor probe N+-BDP at 4.5 hpi (0 min) and FLIM images captured for 3 h and fluorescence lifetimes plotted. Source data EXTENDED DATA FIG.

5 THE MECHANOSENSOR N+-BDP REVEALS SPATIAL VARIATIONS IN MEMBRANE TENSION IN _M. ORYZAE_ APPRESSORIA ON A YIELDING PLANT SURFACE. A, Time course of _M. oryzae_ infection-related-development

on onion epidermis treated with chloroform. Images are representative of _n_ = _3_ independent repeats of the experiment. B, FLIM micrographs of Guy11 at 24 h germinated on onion epidermis

treated with chloroform and stained with the rotor probe N+-BDP. The colour corresponds to the fluorescence lifetime values expressed in nanoseconds, as shown in the key 2–4 ns. C, Dot plot

showing the average fluorescence lifetime for 24hpi Guy11 appressoria developed on onion epidermis. Each dot corresponds to the average fluorescence lifetime obtained for an ROI drawn around

the membrane of an individual appressorium in a 2D FLIM image. Total observations _n_ = 21 appressoria in 3 biological replicates; each biological replicate is colour coded (R1: replicate

1, R2: replicate 2, R3: replicate 3); data are presented as mean ± SEM. Source data EXTENDED DATA FIG. 6 MAPPING SPATIAL VARIATION IN CHEMICAL POLARITY OF THE PLASMA MEMBRANE IN _M. ORYZAE_

APPRESSORIA USING THE SOLVATOCHROMIC PROBE NR12S. A, Chemical structure of NR12S. B,C Schematic illustrations showing the mechanism by which NR12S reports changes in chemical polarity and

lipid phase. D-J, Intensity ratio chemical polarity maps in _M. oryzae_ wild type strain Guy11 4 h appressoria. Images E, F, H and I are magnified areas of 4 h appressoria. The colour scale

translates the intensity ratio values (_n_ = 31 three independent repetitions of the experiment were performed). N+-BDP 4 h and N+-BDP 24 h FLIM images included for comparison. K-P,

Intensity ratio chemical polarity maps of _M. oryzae_ wild type strain Guy11 24 h appressoria. Images I, M, O and P are zoomed in areas of 24 h appressoria, Total observations _n_ = 34

appressoria in 3 biological replicates. Images are representative of _n_ = _3_ independent repeats of the experiment. Images D, G, J, K and N, scale bars = 5 μm, images E, F, H, I, L, M, O

and P scale bars= 1 μm. Image created with BioRender.com. SUPPLEMENTARY INFORMATION REPORTING SUMMARY PEER REVIEW FILE SUPPLEMENTARY VIDEO 1 THREE-DIMENSIONAL FLIM ROTATIONAL MOVIE OF GUY11

4 H APPRESSORIUM. Three-dimensional FLIM of N+-BDP stained 4 h incipient appressoria of Guy11 was performed on a Stellaris 8 FALCON upright scanning confocal microscope. Conidia were

collected from the _M. oryzae_ wild-type Guy11 and inoculated onto glass coverslips. SUPPLEMENTARY VIDEO 2 THREE-DIMENSIONAL FLIM ROTATIONAL MOVIE OF GUY11 7.5 H APPRESSORIUM.

Three-dimensional FLIM of N+-BDP stained Guy11 appressoria at 7.5 h was performed on a Stellaris 8 FALCON upright scanning confocal microscope. Conidia were collected from the _M. oryzae_

wild-type strain Guy11 and inoculated onto glass coverslips. SUPPLEMENTARY VIDEO 3 LIVE CELL IMAGING OF TURGOR GENERATION IN A DEVELOPING GUY11 APPRESSORIUM STAINED WITH THE ROTOR PROBE

N+-BDP 4.5 H 7 HPI. Conidia were collected from a _M. oryzae_ Guy11 and inoculated on glass coverslips. The 4 h appressoria were incubated with 80 µL of N+-BDP rotor probe at 10 µmol−1 in

water for 5 min, washed five times and imaged. SUPPLEMENTARY VIDEO 4 THREE-DIMENSIONAL FLIM ROTATIONAL MOVIE OF A 24 H APPRESSORIUM OF THE _ALB1_ MELANIN-DEFICIENT MUTANT. Three-dimensional

FLIM of _alb1_ 24 h appressoria stained with N+-BDP performed on a Stellaris 8 FALCON upright scanning confocal microscope. Conidia were collected from a _M. oryzae_ wild-type Guy11 and

inoculated onto glass coverslips. SUPPLEMENTARY VIDEO 5 DYNAMIC ASSEMBLY OF A SEPTIN RING IN GUY11 APPRESSORIA. Live cell imaging of septin dynamics during appressorium development in _M.

oryzae_. Movie shows Guy11 expressing Sep5–GFP during infection-related development on a hydrophobic glass coverslip. The movie was captured using a Leica SP8 laser confocal microscope 0–24 

h. The movie is a maximum projection _z_-stack. Frames were captured every 5 min and are displayed at 15 frames s−1. Time scale is in hours:minutes:seconds. Scale bar, 5 μm. SUPPLEMENTARY

VIDEO 6 ABERRANT SEPTIN RING AGGREGATION AND HYPER-MELANIZATION IN THE _∆SLN1_ MUTANT. Live cell imaging of aberrant septin dynamics during appressorium development in _M. oryzae_. Movie

shows the _∆sln1_ mutant expressing Sep5–GFP during infection-related development on hydrophobic glass coverslips. The movie was captured using a Leica SP8 laser confocal microscope 0–24 

hpi. The movie is a maximum projection _z_-stack. Frames were captured every 5 min and are displayed at 15 frames s−1. Time scale is in hours:minutes:seconds. Scale bar, 10 μm. SUPPLEMENTARY

VIDEO 7 THREE-DIMENSIONAL FLIM ROTATIONAL MOVIE OF _∆SLN1_ 24 H APPRESSORIUM Three-dimensional FLIM of N+-BDP stained _∆sln1_ 24 h appressoria was performed on a Stellaris 8 FALCON upright

scanning confocal microscope. Conidia were collected from _M. oryzae_ wild-type Guy11 and inoculated onto glass coverslips. SOURCE DATA SOURCE DATA FIG. 1 Source Data Fig. 1. SOURCE DATA

FIG. 2 Source Data Fig. 2. SOURCE DATA FIG. 3 Source Data Fig. 3. SOURCE DATA FIG. 4 Source Data Fig. 4. SOURCE DATA FIG. 5 Source Data Fig. 5. SOURCE DATA FIG. 6 Source Data Fig. 6. SOURCE

DATA EXTENDED DATA FIG. 3 Source Data Extended Data Fig. 3. SOURCE DATA EXTENDED DATA FIG. 4 Source Data Extended Data Fig. 4. SOURCE DATA EXTENDED DATA FIG. 5 Source Data Extended Data Fig.

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ARTICLE Ryder, L.S., Lopez, S.G., Michels, L. _et al._ A molecular mechanosensor for real-time visualization of appressorium membrane tension in _Magnaporthe oryzae_. _Nat Microbiol_ 8,

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