Play all audios:
Sequencing of proteins is a technically difficult task that typically requires digestion into short peptides before detection and identification. We developed a digestion-free method to
chemically unfold and ‘scan’ full-length proteins through a nanopore, producing electrical fingerprints unique to individual protein molecules that are useful in their identification. Access
through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Access Nature and 54 other
Nature Portfolio journals Get Nature+, our best-value online-access subscription $32.99 / 30 days cancel any time Learn more Subscribe to this journal Receive 12 print issues and online
access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which
are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Alfaro, J. A. et al. The
emerging landscape of single-molecule protein sequencing technologies. _Nat. Methods_ 18, 604–617 (2021). THIS REVIEW ARTICLE PRESENTS THE DEVELOPMENT OF PROTEIN SEQUENCING TECHNOLOGIES.
Article CAS PubMed PubMed Central Google Scholar * Deamer, D., Akeson, M. & Branton, D. Three decades of nanopore sequencing. _Nat. Biotechnol._ 34, 518–524 (2016). THIS REVIEW
ARTICLE SUMMARIZES THREE DECADES OF NANOPORE SEQUENCING. Article CAS PubMed PubMed Central Google Scholar * Yu, L. et al. Stable polymer bilayers for protein channel recordings at high
guanidinium chloride concentrations. _Biophys. J._ 120, 1537–1541 (2021). THIS PAPER REPORTS A SUITABLE POLYMER BILAYER FOR DENATURED PROTEIN SENSING USING A BIOLOGICAL NANOPORE. Article
CAS PubMed PubMed Central Google Scholar * Kang, X., Alibakhshi, M. A. & Wanunu, M. One-pot species release and nanopore detection in a voltage-stable lipid bilayer platform. _Nano
Lett_ 19, 9145–9153 (2019). THIS PAPER REPORTS A WEDGE-ON-PILLAR MEMBRANE SUPPORT FOR NANOPORE EXPERIMENTS. Article CAS PubMed Google Scholar Download references ADDITIONAL INFORMATION
PUBLISHER’S NOTE Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. THIS IS A SUMMARY OF: Yu, L. et al. Unidirectional
single-file transport of full-length proteins through a nanopore. _Nat. Biotechnol_. https://doi.org/10.1038/s41587-022-01598-3 (2023) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT
THIS ARTICLE CITE THIS ARTICLE Protein identification using a digestion-free nanopore approach. _Nat Biotechnol_ 41, 1078–1079 (2023). https://doi.org/10.1038/s41587-022-01599-2 Download
citation * Published: 09 January 2023 * Issue Date: August 2023 * DOI: https://doi.org/10.1038/s41587-022-01599-2 SHARE THIS ARTICLE Anyone you share the following link with will be able to
read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing
initiative