Play all audios:
Prime editing systems hold tremendous promise for the precise correction of pathogenic mutations. We developed a method to tag sequences modified by a prime editor to evaluate its
genome-wide precision for therapeutic applications. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS
Access through your institution Access Nature and 54 other Nature Portfolio journals Get Nature+, our best-value online-access subscription $29.99 / 30 days cancel any time Learn more
Subscribe to this journal Receive 12 print issues and online access $259.00 per year only $21.58 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full
article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs *
Contact customer support REFERENCES * Anzalone, A. V. et al. Search-and-replace genome editing without double-strand breaks or donor DNA. _Nature_ 576, 149–157 (2019). THIS PAPER IS THE
ORIGINAL DESCRIPTION OF A CAS9-BASED PRIME EDITING SYSTEM. Article CAS PubMed PubMed Central Google Scholar * Giannoukos, G. et al. UDiTaS, a genome editing detection method for indels
and genome rearrangements. _BMC Genomics_ 19, 212 (2018). THIS PAPER REPORTS THE UMI-BASED TN5 TAGMENTATION METHODOLOGY. Article PubMed PubMed Central Google Scholar * Tsai, S. Q. et al.
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. _Nat. Biotechnol._ 33, 187–197 (2015). THIS PAPER REPORTS THE GUIDE-SEQ METHOD TO CAPTURE CAS9
NUCLEASE-BASED OFF-TARGET SITES GENOME-WIDE. Article CAS PubMed Google Scholar * Kim, D. Y., Moon, S. B., Ko, J. H., Kim, Y. S. & Kim, D. Unbiased investigation of specificities of
prime editing systems in human cells. _Nucleic Acids Res_ 48, 10576–10589 (2020). THIS PAPER REPORTS A NICKASE-BASED DIGENOME-SEQ (NDIGENOME-SEQ) APPROACH TO IDENTIFY SINGLE-STRAND BREAKS
INDUCED BY CAS9 H840A NICKASE. Article CAS PubMed PubMed Central Google Scholar * Petri, K. et al. CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human
cells. _Nat. Biotechnol._ 40, 189–193 (2021). THIS PAPER REPORTS PRIME EDITING WITH PURIFIED PRIME EDITOR PROTEIN. Article PubMed PubMed Central Google Scholar Download references
ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. THIS IS A SUMMARY OF: Liang,
S.-Q. et al. Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag. _Nat. Methods_ https://doi.org/10.1038/s41592-023-01859-2 (2023) RIGHTS AND PERMISSIONS
Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Capturing prime editor off-target sites within the genome. _Nat Methods_ 20, 801–802 (2023).
https://doi.org/10.1038/s41592-023-01860-9 Download citation * Published: 08 May 2023 * Issue Date: June 2023 * DOI: https://doi.org/10.1038/s41592-023-01860-9 SHARE THIS ARTICLE Anyone you
share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the
Springer Nature SharedIt content-sharing initiative