Author correction: effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate

Author correction: effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate

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Correction to: _Scientific Reports_ https://doi.org/10.1038/s41598-020-60622-1, published online 26 February 2020 The Article contains several typographical errors and omitted References in the Materials and Methods section under the subheading ‘PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufA’ where: “PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515 f (F), 5′-GTGCCAGCMGCCGCGGTAA-3′ and 806r (R), 5′-GGACTACHVHHHTWTCTAAT-3′] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGT-TATCCCTAGAG-3′ primers for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” should read: “PCR amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515f (F), 5′-GTGYCAGCMGCCGCGGTAA-3′1 and 806r (R), 5′-GGACTACNVGGGTWTCTAAT-3′2] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers3 for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGTTATCCCTAGAG-3′ primers4 for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” The omitted References are listed below as References 1–4 respectively. REFERENCES * Parada, A. E., Needham, D. M. & Fuhrman, J. A. Every base matters: Assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. _Environ. Microbiol._ 18(5), 1403–1414 (2016). Article  CAS  Google Scholar  * Apprill, A., McNally, S., Parsons, R. & Weber, L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. _Aquat. Microb. Ecol._ 75(2), 129–137 (2015). Article  Google Scholar  * Stoeck, T. _et al._ Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. _Mol. Ecol._ 19, 21–31 (2010). Article  CAS  Google Scholar  * Sherwood, A. R. & Presting, G. G. Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria 1. _J. Phycol._ 43(3), 605–608 (2007). Article  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Environmental Engineering, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey Hande Ermis & Mahmut Altinbas * PHI Tech Bioinformatics R&D Inc., 41400, Gebze, Kocaeli, Turkey Unzile Guven-Gulhan * Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey Tunahan Cakir Authors * Hande Ermis View author publications You can also search for this author inPubMed Google Scholar * Unzile Guven-Gulhan View author publications You can also search for this author inPubMed Google Scholar * Tunahan Cakir View author publications You can also search for this author inPubMed Google Scholar * Mahmut Altinbas View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Hande Ermis. RIGHTS AND PERMISSIONS OPEN ACCESS This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Ermis, H., Guven-Gulhan, U., Cakir, T. _et al._ Author Correction: Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate. _Sci Rep_ 10, 11963 (2020). https://doi.org/10.1038/s41598-020-69043-6 Download citation * Published: 15 July 2020 * DOI: https://doi.org/10.1038/s41598-020-69043-6 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

Correction to: _Scientific Reports_ https://doi.org/10.1038/s41598-020-60622-1, published online 26 February 2020 The Article contains several typographical errors and omitted References in


the Materials and Methods section under the subheading ‘PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufA’ where: “PCR amplification and sequence analyses of


16 S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different


mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region


primers [515 f (F), 5′-GTGCCAGCMGCCGCGGTAA-3′ and 806r (R), 5′-GGACTACHVHHHTWTCTAAT-3′] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′


primers for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGT-TATCCCTAGAG-3′ primers for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R)


5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” should read: “PCR


amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA


marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were


constructed with universal V4 region primers [515f (F), 5′-GTGYCAGCMGCCGCGGTAA-3′1 and 806r (R), 5′-GGACTACNVGGGTWTCTAAT-3′2] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and


TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers3 for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGTTATCCCTAGAG-3′ primers4 for 23S rDNA, (F)


5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300


paired-end reads).” The omitted References are listed below as References 1–4 respectively. REFERENCES * Parada, A. E., Needham, D. M. & Fuhrman, J. A. Every base matters: Assessing


small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. _Environ. Microbiol._ 18(5), 1403–1414 (2016). Article  CAS  Google Scholar  *


Apprill, A., McNally, S., Parsons, R. & Weber, L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. _Aquat. Microb. Ecol._


75(2), 129–137 (2015). Article  Google Scholar  * Stoeck, T. _et al._ Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic


water. _Mol. Ecol._ 19, 21–31 (2010). Article  CAS  Google Scholar  * Sherwood, A. R. & Presting, G. G. Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and


cyanobacteria 1. _J. Phycol._ 43(3), 605–608 (2007). Article  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Environmental Engineering,


Istanbul Technical University, 34469, Maslak, Istanbul, Turkey Hande Ermis & Mahmut Altinbas * PHI Tech Bioinformatics R&D Inc., 41400, Gebze, Kocaeli, Turkey Unzile Guven-Gulhan *


Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey Tunahan Cakir Authors * Hande Ermis View author publications You can also search for this author


inPubMed Google Scholar * Unzile Guven-Gulhan View author publications You can also search for this author inPubMed Google Scholar * Tunahan Cakir View author publications You can also


search for this author inPubMed Google Scholar * Mahmut Altinbas View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to


Hande Ermis. RIGHTS AND PERMISSIONS OPEN ACCESS This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution


and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if


changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the


material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to


obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and permissions ABOUT THIS ARTICLE CITE THIS


ARTICLE Ermis, H., Guven-Gulhan, U., Cakir, T. _et al._ Author Correction: Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in


anaerobic liquid digestate. _Sci Rep_ 10, 11963 (2020). https://doi.org/10.1038/s41598-020-69043-6 Download citation * Published: 15 July 2020 * DOI:


https://doi.org/10.1038/s41598-020-69043-6 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not


currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative