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ABSTRACT IN this laboratory, investigations designed to purify the diabetogenic principle of ox anterior pituitary tissue, the daily administration of which to the intact dog or cat induces
a condition of diabetes mellitus, have been in progress for some time1-3. Methods involving precipitation in the presence or absence of neutral salts have yielded a potent preparation
capable of inducing the appearance of glycosuria in a normal intact cat when administered in a dose of 4 mgm. protein/day (approximately 0.1 mgm. protein N/kgm. body weight/day) over a
period of four days (graph _a_). We have found similar diabetogenic activity to be exhibited both by purified growth hormone prepared in our laboratory by the method of Li, Evans and
Simpson4 and by the crystalline growth hormone obtained by the ethanol-precipitation method of Wilhelmi, Fishman and Russell5 (see graph b). We have also had the opportunity of testing, in
the intact adult cat, the diabeto-genic activity of growth hormone prepared according to the method of Li _et_ al4 by Dr. C. H. Li of the Institute of Experimental Biology, University of
California (to whom we are grateful for his generous gift), and also of crystalline growth hormone prepared in the laboratories of Armour and Co., Chicago, by the method of Wilhelmi _et
al._5. For the gift of the latter preparation we are indebted to Dr. A. E. Wilhelmi, of the Department of Physiological Chemistry, Yale University, and to Dr. I. M. Bunding, of Armour and
Co., Chicago. Both preparations were highly diabetogenic in the adult intact cat (graphs c and d), and, doubtless, similar activity can be demonstrated in the intact adult dog, since in our
experience the cat and the dog are strictly comparable test animals in such experiments. It will be seen from the graphs that under the conditions of our experiments treatment with growth
hormone caused little or no significant change in the body-weight of adult intact cats. Access through your institution Buy or subscribe This is a preview of subscription content, access via
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* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS FORMATION OF THYROID HORMONE REVEALED BY A CRYO-EM STRUCTURE OF
NATIVE BOVINE THYROGLOBULIN Article Open access 02 May 2022 COMPARATIVE ANALYSIS OF NEUROSTEROID LEVELS IN NORMAL AND ADENOMATOUS HUMAN PITUITARY TISSUE Article Open access 16 May 2025
AUTOANTIBODY MIMICRY OF HORMONE ACTION AT THE THYROTROPIN RECEPTOR Article 08 August 2022 REFERENCES * Young, F. G., _Biochem. J._, 32, 524 (1938); _J. Endocrinol._, 1, 339 (1939). Article
CAS Google Scholar * Reid, E., and Young, F. G., _Biochem. J._, 42, 54 (1948); Reid, E., _Biochem. J._ 44 42 (1949). Google Scholar * Cotes, P. M., Reid, E., and Young, F. G., _J.
Endocrinol._ (in the press). * Li, C. H., Evans, H. M., and Simpson, M. E., _J. Biol. Chem._, 159, 353 (1945). CAS Google Scholar * Wilhelmi, A. E., Fishman, J. B., and Russell, J. A., _J.
Biol. Chem._, 176, 735 (1948). CAS PubMed Google Scholar * Milman, A. E., and Russell, J. A., _Fed. Proc._, 8, 111 (1949). Google Scholar * Ingle, D. J., Li, C. H., and Evans, H. M.,
_Endocrinology_, 39, 32 (1946). Article CAS Google Scholar * Bennett, L. L., and Li, C. H., _Amer. J. Physiol._, 150, 400 (1947), see also Bennett, L. L., and Laundrie, B., _Amer. J.
Physiol._, 155; 18 (1948). Article CAS Google Scholar * Conn, J. W., Louis, L. H., and Wheeler, C. E., _J. Lab. Clin. Med._, 33, 651 (1948); Conn, J. W., Louis, L. H., and Johnston, M.
W., _J. Lab. Clin. Med._, 34, 255 (1949). CAS PubMed Google Scholar * Li, C. H., _Ann. Rev. Biochem._, 16, 291 (1947). Article CAS Google Scholar * Marks, H. P., and Young, F. G.,
_Lancet_, 1, 493 (1940). Article Google Scholar * Shipley, R. A., and Long, C. N. H., _Biochem. J._, 32, 2242 (1938). Article CAS Google Scholar * Young, F. G., _Brit. Med. J._, 2, 393
(1939); _Endocrinology_, 26, 345 (1940). Article CAS Google Scholar * Bonsnes, R. W., and White, A., _Endocrinology_, 26, 990 (1940). Article CAS Google Scholar * Young, F. G.,
_Biochem. J._, 39, 515 (1945). Article CAS Google Scholar * Evans, H. M., Meyer, K., Simpson, M. E., and Reichert, F. L., _Proc. Soc. Exp. Biol. N.Y._, 29, 857 (1932). Article Google
Scholar * Young, F. G., _Brit. Med. J._, 2, 897 (1941); 2, 715 (1944). Article CAS Google Scholar * Young, F. G., _Acta. Med. Scand._ (in the press). * Young, F. G., _Biochem. J._, 32,
513 (1938). Article CAS Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry, University College, London, P. M. COTES, E. REID &
F. G. YOUNG F.R.S. Authors * P. M. COTES View author publications You can also search for this author inPubMed Google Scholar * E. REID View author publications You can also search for
this author inPubMed Google Scholar * F. G. YOUNG F.R.S. View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions
ABOUT THIS ARTICLE CITE THIS ARTICLE COTES, P., REID, E. & YOUNG, F. Diabetogenic Action of Pure Anterior Pituitary Growth Hormone. _Nature_ 164, 209–211 (1949).
https://doi.org/10.1038/164209a0 Download citation * Issue Date: 06 August 1949 * DOI: https://doi.org/10.1038/164209a0 SHARE THIS ARTICLE Anyone you share the following link with will be
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