ABSTRACT Efficient transfection conditions for a number of human, rat and rabbit primary cells and established lines of vascular origin have been determined using a complex of a commercially

available cationic lipid transfection agent (Tfx™-50) and luciferase reporter plasmid constructs. The optimised conditions have also been successfully applied to rabbit carotid arteries in

vivo and a series of human arteries in vitro. The most critical factors influencing the efficiency of gene transfection with this protocol are: DNA concentration; ratio of lipid reagent to

DNA; transfection time and the presence or absence of serum. Immunohistochemical analysis shows that a high percentage of cells (approximately 30–80% dependent on lineage) were transfected

lipid complex will be valuable for molecular studies of genes implicated in cardiovascular diseases and as a possible method of gene delivery with therapeutic intent. Access through your

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access 26 February 2024 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Thrombosis Research Institute, Emmanuel Kaye Building, London, UK M-C Keogh, D Chen, F Lupu, N Shaper, JF Schmitt, VV

Kakkar & NR Lemoine * Imperial Cancer Research Fund, Molecular Oncology Unit, Hammersmith Hospital, London, UK NR Lemoine Authors * M-C Keogh View author publications You can also search

for this author inPubMed Google Scholar * D Chen View author publications You can also search for this author inPubMed Google Scholar * F Lupu View author publications You can also search

for this author inPubMed Google Scholar * N Shaper View author publications You can also search for this author inPubMed Google Scholar * JF Schmitt View author publications You can also

search for this author inPubMed Google Scholar * VV Kakkar View author publications You can also search for this author inPubMed Google Scholar * NR Lemoine View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Keogh, MC., Chen, D., Lupu, F. _et al._ High

efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid–DNA complex. _Gene Ther_ 4, 162–171 (1997). https://doi.org/10.1038/sj.gt.3300374

Download citation * Received: 19 August 1996 * Accepted: 05 November 1996 * Issue Date: 01 February 1997 * DOI: https://doi.org/10.1038/sj.gt.3300374 SHARE THIS ARTICLE Anyone you share the

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Nature SharedIt content-sharing initiative KEYWORDS * gene therapy * cationic liposomes * luciferase * Tfx™-50 * transfection * vascular tissues