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ABSTRACT In most plants flower pigments derive from the flavonoid biosynthesis pathway. Consistent with this pathway in _Petunia hybrida_ the key enzyme in flavonoid synthesis, chalcone
synthase, is synthesized in the flower corolla, tube and anthers1. Here we show that constitutive expression of an 'anti-sense' chalcone synthase gene in transgenic petunia and
tobacco plants results, with high frequency, in an altered flower pigmentation due to a reduction in levels of both the messenger RNA for the enzyme and the enzyme itself. The pattern of
pigmentation varies among flowers of different transgenic plants, indicating that the activity of the anti-sense gene is influenced by DNA sequences that border its site of insertion in both
a quantitative and a qualitative way. Backcrossing experiments show that the different pigmentation phenotypes resulting from the expression of anti-sense chalcone synthese gene(s) are
stably inherited. These data establish that secondary metabolism in plants can be manipulated using transgenic plants that constitutively synthesize anti-sense RNA. Access through your
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BEING VIEWED BY OTHERS SUPPRESSION OF CHORISMATE SYNTHASE, WHICH IS LOCALIZED IN CHLOROPLASTS AND PEROXISOMES, RESULTS IN ABNORMAL FLOWER DEVELOPMENT AND ANTHOCYANIN REDUCTION IN PETUNIA
Article Open access 02 July 2020 THE PLANT ALCR-_PALCA_ ETHANOL-INDUCIBLE SYSTEM DISPLAYS GROSS GROWTH ARTEFACTS INDEPENDENTLY OF DOWNSTREAM _PALCA-_REGULATED INDUCIBLE CONSTRUCTS Article
Open access 25 January 2021 CRISPR/CAS9-MEDIATED TARGETED MUTATION REVEALS A ROLE FOR _AN4_ RATHER THAN _DPL_ IN REGULATING VENATION FORMATION IN THE COROLLA TUBE OF _PETUNIA HYBRIDA_
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AUTHORS AND AFFILIATIONS * Department of Applied Genetics, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands Alexander R. van der Krol, Peter E. Lenting, Jetty
Veenstra, Ingrid M. van der Meer, Ronald E. Koes, Anton G. M. Gerats, Joseph N. M. Mol & Antoine R. Stuitje Authors * Alexander R. van der Krol View author publications You can also
search for this author inPubMed Google Scholar * Peter E. Lenting View author publications You can also search for this author inPubMed Google Scholar * Jetty Veenstra View author
publications You can also search for this author inPubMed Google Scholar * Ingrid M. van der Meer View author publications You can also search for this author inPubMed Google Scholar *
Ronald E. Koes View author publications You can also search for this author inPubMed Google Scholar * Anton G. M. Gerats View author publications You can also search for this author inPubMed
Google Scholar * Joseph N. M. Mol View author publications You can also search for this author inPubMed Google Scholar * Antoine R. Stuitje View author publications You can also search for
this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE van der Krol, A., Lenting, P., Veenstra, J. _et al._ An anti-sense
chalcone synthase gene in transgenic plants inhibits flower pigmentation. _Nature_ 333, 866–869 (1988). https://doi.org/10.1038/333866a0 Download citation * Received: 25 January 1988 *
Accepted: 04 May 1988 * Issue Date: 30 June 1988 * DOI: https://doi.org/10.1038/333866a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get
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