ABSTRACT It is over a decade since the first demonstration that mouse embryonic stem cells could be used to transfer a predetermined genetic modification to a whole animal1. The extension of

this technique to other mammalian species, particularly livestock, might bring numerous biomedical benefits, for example, ablation of xenoreactive transplantation antigens, inactivation of

genes responsible for neuropathogenic disease and precise placement of transgenes designed to produce proteins for human therapy. Gene targeting has not yet been achieved in mammals other

than mice, however, because functional embryonic stem cells have not been derived. Nuclear transfer from cultured somatic cells provides an alternative means of cell-mediated

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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS AN OPTIMIZED CULTURE SYSTEM FOR EFFICIENT DERIVATION OF PORCINE EXPANDED POTENTIAL STEM CELLS

FROM PREIMPLANTATION EMBRYOS AND BY REPROGRAMMING SOMATIC CELLS Article 20 March 2024 BLASTOCYST COMPLEMENTATION USING _PRDM14_-DEFICIENT RATS ENABLES EFFICIENT GERMLINE TRANSMISSION AND

GENERATION OF FUNCTIONAL MOUSE SPERMATIDS IN RATS Article Open access 26 February 2021 EFFICIENT MARMOSET GENOME ENGINEERING BY AUTOLOGOUS EMBRYO TRANSFER AND CRISPR/CAS9 TECHNOLOGY Article

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references ACKNOWLEDGEMENTS We would like to acknowledge the contributions of Y. Gibson and K. Mycock for embryo manipulation; E. Emslie and L. Hutchison for molecular biology technical

assistance; T. Johnston for protein analysis; and the PPL Therapeutics large animal team for animal husbandry and veterinary procedures. We thank I. Garner and D. Ayares for useful

discussions. AUTHOR INFORMATION Author notes * K. H. S. Campbell Present address: Division of Animal Physiology, University of Nottingham, School of Biological Sciences, Loughborough, LE12

5RD, UK * Correspondence and requests for materials should be addressed to A.J.K. AUTHORS AND AFFILIATIONS * PPL Therapeutics Ltd, Roslin, Edinburgh, EH25 9PP, UK K. J. McCreath, J.

Howcroft, K. H. S. Campbell, A. Colman, A. E. Schnieke & A.J. Kind Authors * K. J. McCreath View author publications You can also search for this author inPubMed Google Scholar * J.

Howcroft View author publications You can also search for this author inPubMed Google Scholar * K. H. S. Campbell View author publications You can also search for this author inPubMed Google

Scholar * A. Colman View author publications You can also search for this author inPubMed Google Scholar * A. E. Schnieke View author publications You can also search for this author

inPubMed Google Scholar * A.J. Kind View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to A.J. Kind. RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE McCreath, K., Howcroft, J., Campbell, K. _et al._ Production of gene-targeted sheep by nuclear transfer from

cultured somatic cells. _Nature_ 405, 1066–1069 (2000). https://doi.org/10.1038/35016604 Download citation * Received: 28 January 2000 * Accepted: 05 May 2000 * Issue Date: 29 June 2000 *

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