Mechanosensitive channels transduce osmosensitivity in supraoptic neurons

Mechanosensitive channels transduce osmosensitivity in supraoptic neurons

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ABSTRACT VASOPRESSIN is a peptide hormone synthesized by neurons of the supraoptic and paraventricular nuclei, which project axon terminals to the neurohypophysis. Consistent with its antidiuretic properties, vasopressin release rises as a function of plasma osmolality1–3, a response that results from accelerated action potential discharge4–6. Previous studies have shown that increases in fluid osmolality depolarize supraoptic neurons in the absence of synaptic transmission7–9, suggesting that these cells behave as intrinsic osmoreceptors. The mechanism by which changes in osmolality are transduced into an electrical signal is unknown, however. Here we report that changes in cell volume accompany physiological variations in fluid osmolality and that these modulate the activity of mechanosensitive cation channels in a way that is consistent with the macroscopic regulation of membrane voltage and action potential discharge. These findings define a function for stretch-inactivated channels in mammalian central neurons. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SODIUM REGULATES CLOCK TIME AND OUTPUT VIA AN EXCITATORY GABAERGIC PATHWAY Article 08 July 2020 ANOCTAMIN-1 IS A CORE COMPONENT OF A MECHANOSENSORY ANION CHANNEL COMPLEX IN _C. ELEGANS_ Article Open access 16 February 2025 STRUCTURAL, MOLECULAR AND DEVELOPMENTAL EVIDENCE FOR CELL-TYPE DIVERSITY IN CNIDARIAN MECHANOSENSORY NEURONS Article Open access 10 February 2025 REFERENCES * Verney, E. B. _Proc. R. Soc._ B135, 25–106 (1947). ADS  CAS  Google Scholar  * Robertson, G. L., Shelton, R. L. & Athar, S. _Kidney Int._ 10, 25–37 (1976). Article  CAS  Google Scholar  * Dunn, F. L., Brennan, T. J., Nelson, A. E. & Robertson, G. L. _J. clin. Invest._ 52, 3212–3219 (1973). Article  CAS  Google Scholar  * Brimble, M. J. & Dyball, R. E. J. _J. Physiol._ 271, 253–272 (1977). Article  CAS  Google Scholar  * Lawrence, D. & Pittman, Q. J. _Brain Res._ 341, 176–183 (1985). Article  CAS  Google Scholar  * Poulain, D. A. & Wakerley, J. B. _Neuroscience_ 7, 773–808 (1982). Article  CAS  Google Scholar  * Mason, W. T. _Nature_ 287, 154–157 (1980). Article  ADS  CAS  Google Scholar  * Bourque, C. W. _J. Physiol._ 417, 263–277 (1989). Article  CAS  Google Scholar  * Oliet, S. H. R. & Bourque, C. W. _J. Physiol._ 455, 291–306 (1992). Article  CAS  Google Scholar  * Darrow, D. C. & Yannet, H. _J. clin. Invest._ 14, 266–275 (1935). Article  CAS  Google Scholar  * Morris, C. E. _J. Membr. Biol._ 113, 93–107 (1990). Article  CAS  Google Scholar  * Sokabe, M. & Sachs, F. in _Advances in Comparative and Environmental Physiology_ 10 (ed. Ito, F.) 55–77 (Springer, Berlin, 1992). Google Scholar  * Morris, C. E. & Sigurdson, W. J. _Science_ 243, 807–809 (1989). Article  ADS  CAS  Google Scholar  * French, A. S. _A. Rev. Physiol._ 54, 135–152 (1992). Article  CAS  Google Scholar  * Morris, C. E. & Horn, R. _Science_ 251, 1246–1249 (1991). Article  ADS  CAS  Google Scholar  * Leng, G., Mason, W. T. & Dyer, R. G. _Neuroendocrinology_ 34, 75–82 (1982). Article  CAS  Google Scholar  * Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. _Pflügers Archs_ 321, 85–100 (1981). Article  Google Scholar  * Brimble, M. J., Dyball, R. E. J. & Forsling, M. L. _J. Physiol._ 278, 69–78 (1978). Article  CAS  Google Scholar  * Friedman, J. E. & Haddad, G. G. _J. Neurosci._ 13, 63–72 (1993). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Centre for Research in Neuroscience, Montreal General Hospital and McGill University, 1650 Cedar Avenue, Montreal, PQ, H3G 1A4, Canada Stéphane H. R. Oliet & Charles Bourque Authors * Stéphane H. R. Oliet View author publications You can also search for this author inPubMed Google Scholar * Charles Bourque View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Oliet, S., Bourque, C. Mechanosensitive channels transduce osmosensitivity in supraoptic neurons. _Nature_ 364, 341–343 (1993). https://doi.org/10.1038/364341a0 Download citation * Received: 08 March 1993 * Accepted: 13 April 1993 * Issue Date: 22 July 1993 * DOI: https://doi.org/10.1038/364341a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT VASOPRESSIN is a peptide hormone synthesized by neurons of the supraoptic and paraventricular nuclei, which project axon terminals to the neurohypophysis. Consistent with its


antidiuretic properties, vasopressin release rises as a function of plasma osmolality1–3, a response that results from accelerated action potential discharge4–6. Previous studies have shown


that increases in fluid osmolality depolarize supraoptic neurons in the absence of synaptic transmission7–9, suggesting that these cells behave as intrinsic osmoreceptors. The mechanism by


which changes in osmolality are transduced into an electrical signal is unknown, however. Here we report that changes in cell volume accompany physiological variations in fluid osmolality


and that these modulate the activity of mechanosensitive cation channels in a way that is consistent with the macroscopic regulation of membrane voltage and action potential discharge. These


findings define a function for stretch-inactivated channels in mammalian central neurons. Access through your institution Buy or subscribe This is a preview of subscription content, access


via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy


this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: *


Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SODIUM REGULATES CLOCK TIME AND OUTPUT VIA AN EXCITATORY


GABAERGIC PATHWAY Article 08 July 2020 ANOCTAMIN-1 IS A CORE COMPONENT OF A MECHANOSENSORY ANION CHANNEL COMPLEX IN _C. ELEGANS_ Article Open access 16 February 2025 STRUCTURAL, MOLECULAR


AND DEVELOPMENTAL EVIDENCE FOR CELL-TYPE DIVERSITY IN CNIDARIAN MECHANOSENSORY NEURONS Article Open access 10 February 2025 REFERENCES * Verney, E. B. _Proc. R. Soc._ B135, 25–106 (1947).


ADS  CAS  Google Scholar  * Robertson, G. L., Shelton, R. L. & Athar, S. _Kidney Int._ 10, 25–37 (1976). Article  CAS  Google Scholar  * Dunn, F. L., Brennan, T. J., Nelson, A. E. &


Robertson, G. L. _J. clin. Invest._ 52, 3212–3219 (1973). Article  CAS  Google Scholar  * Brimble, M. J. & Dyball, R. E. J. _J. Physiol._ 271, 253–272 (1977). Article  CAS  Google


Scholar  * Lawrence, D. & Pittman, Q. J. _Brain Res._ 341, 176–183 (1985). Article  CAS  Google Scholar  * Poulain, D. A. & Wakerley, J. B. _Neuroscience_ 7, 773–808 (1982). Article


  CAS  Google Scholar  * Mason, W. T. _Nature_ 287, 154–157 (1980). Article  ADS  CAS  Google Scholar  * Bourque, C. W. _J. Physiol._ 417, 263–277 (1989). Article  CAS  Google Scholar  *


Oliet, S. H. R. & Bourque, C. W. _J. Physiol._ 455, 291–306 (1992). Article  CAS  Google Scholar  * Darrow, D. C. & Yannet, H. _J. clin. Invest._ 14, 266–275 (1935). Article  CAS 


Google Scholar  * Morris, C. E. _J. Membr. Biol._ 113, 93–107 (1990). Article  CAS  Google Scholar  * Sokabe, M. & Sachs, F. in _Advances in Comparative and Environmental Physiology_ 10


(ed. Ito, F.) 55–77 (Springer, Berlin, 1992). Google Scholar  * Morris, C. E. & Sigurdson, W. J. _Science_ 243, 807–809 (1989). Article  ADS  CAS  Google Scholar  * French, A. S. _A.


Rev. Physiol._ 54, 135–152 (1992). Article  CAS  Google Scholar  * Morris, C. E. & Horn, R. _Science_ 251, 1246–1249 (1991). Article  ADS  CAS  Google Scholar  * Leng, G., Mason, W. T.


& Dyer, R. G. _Neuroendocrinology_ 34, 75–82 (1982). Article  CAS  Google Scholar  * Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. _Pflügers Archs_ 321, 85–100


(1981). Article  Google Scholar  * Brimble, M. J., Dyball, R. E. J. & Forsling, M. L. _J. Physiol._ 278, 69–78 (1978). Article  CAS  Google Scholar  * Friedman, J. E. & Haddad, G. G.


_J. Neurosci._ 13, 63–72 (1993). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Centre for Research in Neuroscience, Montreal General


Hospital and McGill University, 1650 Cedar Avenue, Montreal, PQ, H3G 1A4, Canada Stéphane H. R. Oliet & Charles Bourque Authors * Stéphane H. R. Oliet View author publications You can


also search for this author inPubMed Google Scholar * Charles Bourque View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and


permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Oliet, S., Bourque, C. Mechanosensitive channels transduce osmosensitivity in supraoptic neurons. _Nature_ 364, 341–343 (1993).


https://doi.org/10.1038/364341a0 Download citation * Received: 08 March 1993 * Accepted: 13 April 1993 * Issue Date: 22 July 1993 * DOI: https://doi.org/10.1038/364341a0 SHARE THIS ARTICLE


Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided


by the Springer Nature SharedIt content-sharing initiative