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ABSTRACT Evaluation of immunotherapy strategies in mouse models of carcinoma is hampered by the limited number of known murine tumor antigens (Ags). Although tumor Ags can be identified
based on cytotoxic T-cell activation, this approach is not readily accomplished for many tumor types. We applied an alternative strategy based on a humoral immune response, SEREX, to the
identification of tumor Ags in the murine colon adenocarcinoma cell line MC38. Immunization of syngeneic C57BL/6 mice with MC38 cells by three different methods induced a protective immune
response with concomitant production of anti-MC38 antibodies. Immunoscreening of an MC38-derived expression library resulted in the identification of the endogenous ecotropic leukemia virus
envelope (env) protein and the murine ATRX protein as candidate tumor Ags. Northern blot analysis demonstrated high levels of expression of the _env_ transcript in MC38 cells and in several
other murine tumor cell lines, whereas expression in normal colonic epithelium was absent. _ATRX_ was found to be variably expressed in tumor cell lines and in normal tissue. Further
analysis of the expressed _env_ sequence indicated that it represents a nonmutated tumor Ag. Polynucleotide immunization with DNA encoding the env polypeptide resulted in strong and specific
antibody responses to this self Ag in all immunized mice. Thus, SEREX offers a rapid means of identifying tumor Ags in murine cancer models. Access through your institution Buy or subscribe
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Open access 11 May 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Gene Therapy Center, Birmingham, 35294, Alabama Tracy A Hampton, David T Curiel & Theresa V Strong * Division of
Hematology/Oncology, Department of Medicine, Birmingham, 35294, Alabama Tracy A Hampton, Robert M Conry, M B Khazaeli, Denise R Shaw, David T Curiel, Albert F LoBuglio & Theresa V Strong
* Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, 35294, Alabama Tracy A Hampton & Theresa V Strong Authors * Tracy A Hampton View
author publications You can also search for this author inPubMed Google Scholar * Robert M Conry View author publications You can also search for this author inPubMed Google Scholar * M B
Khazaeli View author publications You can also search for this author inPubMed Google Scholar * Denise R Shaw View author publications You can also search for this author inPubMed Google
Scholar * David T Curiel View author publications You can also search for this author inPubMed Google Scholar * Albert F LoBuglio View author publications You can also search for this author
inPubMed Google Scholar * Theresa V Strong View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Theresa V Strong.
RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hampton, T., Conry, R., Khazaeli, M. _et al._ SEREX analysis for tumor antigen identification in a mouse
model of adenocarcinoma. _Cancer Gene Ther_ 7, 446–455 (2000). https://doi.org/10.1038/sj.cgt.7700124 Download citation * Received: 13 April 1999 * Accepted: 31 May 1999 * Published: 24 May
2000 * Issue Date: 01 March 2000 * DOI: https://doi.org/10.1038/sj.cgt.7700124 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable
link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * Tumor antigen *
endogenous retrovirus * antibody * adenocarcinoma * ATRX.