ABSTRACT Despite successes in animals, cytokine gene expression selectively in human tumors is difficult to achieve owing to lack of efficient delivery methods. Since interleukin

(IL)-2-activated natural killer (A-NK) and phytohemagglutinin and IL-2 activated killer T (T-LAK) cells, as previously demonstrated, localize and accumulate in murine lung tumor metastases

following adoptive transfer, we transduced them to test their ability to deliver products of genes selectively to tumors. Assessments of transduction efficiency _in vitro_ demonstrated that

adenoviral transduction consistently resulted in high (>60%) transduction rates and substantial expression of transgenes such as GFP, Red2, luciferase, _β_-galactosidase and mIL-12 for at

significantly greater antitumor response than non-transduced A-NK cells. Thus, adoptive transfer of A-NK and T-LAK cells represents an efficient method for targeting products of genes to

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customer support SIMILAR CONTENT BEING VIEWED BY OTHERS REDIRECTING NK CELLS TO THE LYMPH NODES TO AUGMENT THEIR LYMPHOMA-TARGETING CAPACITY Article Open access 20 May 2024 AN ONCOLYTIC

VIRUS–T CELL CHIMERA FOR CANCER IMMUNOTHERAPY Article 09 February 2024 ONCOLYTIC VIRUS PROMOTES TUMOR-REACTIVE INFILTRATING LYMPHOCYTES FOR ADOPTIVE CELL THERAPY Article 07 July 2020

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  PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS This study was supported by grants from the US-NIH (Grants No. R01CA104560 and RO1CA87672) and the American Cancer Society

(Grant No. RPG-00-221-01-CDD). We thank Ms Patricia Rice and Mrs. Lisa Bailey for excellent technical assistance. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Immunology,

University of Pittsburgh, Pittsburgh, PA, USA S Goding, Q Yang & P H Basse * University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA S Goding, Q Yang, P

D Robbins & P H Basse * Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Z Mi & P D Robbins * Pittsburgh, PA, USA Z Mi & P D Robbins Authors * S

Goding View author publications You can also search for this author inPubMed Google Scholar * Q Yang View author publications You can also search for this author inPubMed Google Scholar * Z

Mi View author publications You can also search for this author inPubMed Google Scholar * P D Robbins View author publications You can also search for this author inPubMed Google Scholar * P

H Basse View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to P H Basse. RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Goding, S., Yang, Q., Mi, Z. _et al._ Targeting of products of genes to tumor sites using adoptively transferred A-NK and T-LAK cells.

_Cancer Gene Ther_ 14, 441–450 (2007). https://doi.org/10.1038/sj.cgt.7701019 Download citation * Received: 12 November 2005 * Revised: 02 September 2006 * Accepted: 08 October 2006 *

Published: 02 February 2007 * Issue Date: May 2007 * DOI: https://doi.org/10.1038/sj.cgt.7701019 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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KEYWORDS * adenovirus * A-NK cell * T-LAK cell * tumor localization * B16 melanoma