Hla class i gene expression on human primary tumours and autologous metastases: demonstration of selective losses of hla antigens on colorectal, gastric and laryngeal carcinomas

Hla class i gene expression on human primary tumours and autologous metastases: demonstration of selective losses of hla antigens on colorectal, gastric and laryngeal carcinomas

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ABSTRACT The expression of HLA class I antigens was studied in 99 primary tumour (colorectal, gastric and laryngeal carcinomas) and 57 autologous metastases using immunohistological techniques and monoclonal antibodies against class I monomorphic determinants, HLA-B isotypic determinants and HLA polymorphic determinants. Fourteen per cent of colorectal, 9.6% of gastric and 20% of laryngeal carcinomas completely lacked class I molecules. Selective losses of HLA-B antigens were also detected in 8.8, 3.4 and 5.8% of these tumours respectively. Taking into account complete and selective loss of HLA-B the average alteration in the class I molecules expression totalled 21%. The comparison of class I expression between primary tumours and autologous metastases showed differences in 24% of the patients. These differences consisted mainly in a decrease of class I expression by metastases. Nevertheless, four types of divergence were detected in laryngeal carcinomas, namely: +/-, +/+, -/+, -/-. In addition, a clear correlation between degree of differentiation and class I expression was observed in laryngeal tumours. Finally, when class I gene RFLPs were compared with DNA from 15 tumours and autologous normal mucosa or peripheral lymphocytes, no differences were detected between these samples. SIMILAR CONTENT BEING VIEWED BY OTHERS HLA CLASS I LOSS IN COLORECTAL CANCER: IMPLICATIONS FOR IMMUNE ESCAPE AND IMMUNOTHERAPY Article 20 January 2021 MHC CLASS I LOSS IN ENDOMETRIAL CARCINOMA: A POTENTIAL RESISTANCE MECHANISM TO IMMUNE CHECKPOINT INHIBITION Article 03 October 2020 GENETIC IMMUNE ESCAPE LANDSCAPE IN PRIMARY AND METASTATIC CANCER Article Open access 10 May 2023 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada, Spain MA López-Nevot Authors * MA López-Nevot View author publications You can also search for this author inPubMed Google Scholar * F Esteban View author publications You can also search for this author inPubMed Google Scholar * A Ferrón View author publications You can also search for this author inPubMed Google Scholar * J Gutiérrez View author publications You can also search for this author inPubMed Google Scholar * MR Oliva View author publications You can also search for this author inPubMed Google Scholar * C Romero View author publications You can also search for this author inPubMed Google Scholar * C Huelin View author publications You can also search for this author inPubMed Google Scholar * F Ruiz-Cabello View author publications You can also search for this author inPubMed Google Scholar * F Garrido View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE López-Nevot, M., Esteban, F., Ferrón, A. _et al._ HLA class I gene expression on human primary tumours and autologous metastases: demonstration of selective losses of HLA antigens on colorectal, gastric and laryngeal carcinomas. _Br J Cancer_ 59, 221–226 (1989). https://doi.org/10.1038/bjc.1989.45 Download citation * Issue Date: 01 February 1989 * DOI: https://doi.org/10.1038/bjc.1989.45 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT The expression of HLA class I antigens was studied in 99 primary tumour (colorectal, gastric and laryngeal carcinomas) and 57 autologous metastases using immunohistological


techniques and monoclonal antibodies against class I monomorphic determinants, HLA-B isotypic determinants and HLA polymorphic determinants. Fourteen per cent of colorectal, 9.6% of gastric


and 20% of laryngeal carcinomas completely lacked class I molecules. Selective losses of HLA-B antigens were also detected in 8.8, 3.4 and 5.8% of these tumours respectively. Taking into


account complete and selective loss of HLA-B the average alteration in the class I molecules expression totalled 21%. The comparison of class I expression between primary tumours and


autologous metastases showed differences in 24% of the patients. These differences consisted mainly in a decrease of class I expression by metastases. Nevertheless, four types of divergence


were detected in laryngeal carcinomas, namely: +/-, +/+, -/+, -/-. In addition, a clear correlation between degree of differentiation and class I expression was observed in laryngeal


tumours. Finally, when class I gene RFLPs were compared with DNA from 15 tumours and autologous normal mucosa or peripheral lymphocytes, no differences were detected between these samples.


SIMILAR CONTENT BEING VIEWED BY OTHERS HLA CLASS I LOSS IN COLORECTAL CANCER: IMPLICATIONS FOR IMMUNE ESCAPE AND IMMUNOTHERAPY Article 20 January 2021 MHC CLASS I LOSS IN ENDOMETRIAL


CARCINOMA: A POTENTIAL RESISTANCE MECHANISM TO IMMUNE CHECKPOINT INHIBITION Article 03 October 2020 GENETIC IMMUNE ESCAPE LANDSCAPE IN PRIMARY AND METASTATIC CANCER Article Open access 10


May 2023 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada, Spain MA López-Nevot Authors * MA López-Nevot View


author publications You can also search for this author inPubMed Google Scholar * F Esteban View author publications You can also search for this author inPubMed Google Scholar * A Ferrón


View author publications You can also search for this author inPubMed Google Scholar * J Gutiérrez View author publications You can also search for this author inPubMed Google Scholar * MR


Oliva View author publications You can also search for this author inPubMed Google Scholar * C Romero View author publications You can also search for this author inPubMed Google Scholar * C


Huelin View author publications You can also search for this author inPubMed Google Scholar * F Ruiz-Cabello View author publications You can also search for this author inPubMed Google


Scholar * F Garrido View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE


López-Nevot, M., Esteban, F., Ferrón, A. _et al._ HLA class I gene expression on human primary tumours and autologous metastases: demonstration of selective losses of HLA antigens on


colorectal, gastric and laryngeal carcinomas. _Br J Cancer_ 59, 221–226 (1989). https://doi.org/10.1038/bjc.1989.45 Download citation * Issue Date: 01 February 1989 * DOI:


https://doi.org/10.1038/bjc.1989.45 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative