ABSTRACT BACKGROUND/OBJECTIVES: The aim was to examine the association of genetic risk scores (GRSs) of vitamin B12 and folate-associated variants with blood pressure and lipids.

SUBJECTS/METHODS: The study included 12 532 adults from three population-based studies (Inter99, Health2006 and Dan-MONICA10) conducted in Denmark. GRSs were calculated by summarising the

number of vitamin B12 and folate increasing alleles. Weighted GRSs were calculated as the sum of weights for each allele corresponding to genetic effects sizes. RESULTS: GRSs for serum

vitamin B12 and folate were associated with serum vitamin B12 and folate, respectively. The β coefficients (95% confidence interval (CI), _P-_value) for regression of log-transformed serum

concentrations (β coefficient (95% CI, _P_-value) for regression of log-transformed HDL on the weighted GRSs, 0.081 (0.015, 0.148), _P_=0.017), but not with blood pressure, triglyceride, and

low-density lipoprotein and total cholesterol levels. CONCLUSIONS: GRSs were not associated with blood pressure and lipid levels, except for an association between the GRS for folate and

HDL cholesterol. Further studies are needed to determine whether a causal association between folate and HDL cholesterol exists. Access through your institution Buy or subscribe This is a

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understanding environmental determinants of disease? _Int J Epidemiol_ 2003; 32: 1–22. Article  Google Scholar  Download references ACKNOWLEDGEMENTS The present study was supported by grants

from the Health Insurance Foundation (grant No. 2010 B 131), the Danish Agency for Science Technology and Innovation (grant No. 2101-06-0065), the Lundbeck Foundation Center for Applied

Medical Genomics in Personalised Disease Prediction, Prevention and Care (LuCamp; http://www.lucamp.org) and The Novo Nordisk Foundation Center for Basic Metabolic Research, which is an

independent research centre at the University of Copenhagen partially funded by an unrestricted donation from the Novo Nordisk Foundation (http://www.metabol.ku.dk). Tea Skaaby was supported

by a grant from the Lundbeck Foundation (Grant number R165-2013-15410). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Research Centre for Prevention and Health, The Capital Region of

Denmark, Glostrup University Hospital, Glostrup, Denmark L L N Husemoen, T Skaaby, B H Thuesen & A Linneberg * The Novo Nordisk Foundation Center for Basic Metabolic Research, University

of Copenhagen, Copenhagen, Denmark N Grarup, C H Sandholt, T Hansen & O Pedersen * University of Southern Denmark, Odense, Denmark T Hansen * University of Copenhagen, Copenhagen,

Denmark A Linneberg * Department of Clinical Experimental Research, Rigshospitalet, Glostrup, Copenhagen, Denmark A Linneberg Authors * L L N Husemoen View author publications You can also

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AUTHOR Correspondence to L L N Husemoen. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no conflict of interest. ADDITIONAL INFORMATION Supplementary Information accompanies

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SUPPLEMENTARY TABLE 4 (DOC 31 KB) SUPPLEMENTARY TABLE 5 (DOC 30 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Husemoen, L., Skaaby, T., Thuesen, B.

_et al._ Mendelian randomisation study of the associations of vitamin B12 and folate genetic risk scores with blood pressure and fasting serum lipid levels in three Danish population-based

studies. _Eur J Clin Nutr_ 70, 613–619 (2016). https://doi.org/10.1038/ejcn.2016.5 Download citation * Received: 04 May 2015 * Revised: 28 December 2015 * Accepted: 27 January 2016 *

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