Play all audios:
ABSTRACT Cytotoxic effect of either cisplatin or p53 gene transfection of lung cancer cells may be different depending on the p53 status of cells. We investigated cytotoxic effects on the
combined treatment of cisplatin and adenovirus mediated p53 gene transfer (Avp53) in both H460 and H1299 cells in vitro. The results showed the highest numbers of apoptotic cells in both
H460 and H1299 cells following the combined treatment regardless of p53 status in comparison with either cisplatin or Avp53 alone. The expression levels of p53, p21, Bax and ICE were
examined to understand a possible cellular signal path of the combined treatment. In western analyses, the patterns of phosphorylated p53 protein were different between Avp53 and combined
treatment. The expressions of p21 and Bax were increased in combined treatment, whereas the cleaved form of ICE (20 kD) was not detected. These results suggest that cisplatin induced p53
protein phosphorylation and may activate the downstream of p53 gene expression such as p21 and Bax. The enhanced apoptosis of lung cancer cells by the combined treatment may be useful in the
development of clinical therapeutic modality of lung tumors. SIMILAR CONTENT BEING VIEWED BY OTHERS AN MDM2 INHIBITOR ACHIEVES SYNERGISTIC CYTOTOXIC EFFECTS WITH ADENOVIRUSES LACKING
_E1B55KDA_ GENE ON MESOTHELIOMA WITH THE WILD-TYPE _P53_ THROUGH AUGMENTING NFI EXPRESSION Article Open access 02 July 2021 ONCOLYTIC ADENOVIRUS ENCODING LHPP EXERTS POTENT ANTITUMOR EFFECT
IN LUNG CANCER Article Open access 07 June 2024 PB01 SUPPRESSES RADIO-RESISTANCE BY REGULATING ATR SIGNALING IN HUMAN NON-SMALL-CELL LUNG CANCER CELLS Article Open access 08 June 2021
ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, Korea Ji Hyung Choi Authors * Ji Hyung Choi View
author publications You can also search for this author inPubMed Google Scholar * Kwang Sung Ahn View author publications You can also search for this author inPubMed Google Scholar *
Jhingook Kim View author publications You can also search for this author inPubMed Google Scholar * Young Sook Hong View author publications You can also search for this author inPubMed
Google Scholar RIGHTS AND PERMISSIONS This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License
(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Choi, J., Ahn, K., Kim, J. _et al._ Enhanced induction of Bax gene expression in H460 and H1299 cells with the combined
treatment of cisplatin and adenovirus mediated wt-p53 gene transfer. _Exp Mol Med_ 32, 23–28 (2000). https://doi.org/10.1038/emm.2000.5 Download citation * Published: 01 March 2000 * Issue
Date: 01 March 2000 * DOI: https://doi.org/10.1038/emm.2000.5 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a
shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * apoptosis * Avp53 * Bax *
cisplatin * combined treatment