ABSTRACT Identification of biomaterials that support appropriate cellular attachment, proliferation and gene expression patterns is critical for tissue engineering and cell therapy. Here we

describe an approach for rapid, nanoliter-scale synthesis of biomaterials and characterization of their interactions with cells. We simultaneously characterize over 1,700 human embryonic

stem cell–material interactions and identify a host of unexpected materials effects that offer new levels of control over human embryonic stem cell behavior. Access through your institution

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ENGINEERING PROGRAMMABLE MATERIAL-TO-CELL PATHWAYS VIA SYNTHETIC NOTCH RECEPTORS TO SPATIALLY CONTROL DIFFERENTIATION IN MULTICELLULAR CONSTRUCTS Article Open access 13 July 2024 REFERENCES

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Google Scholar  Download references ACKNOWLEDGEMENTS The authors would like to thank David Putnam, David Lynn, David Lavan and Daniel Kohane for helpful advice, Mara Macdonald for help with

cell culture and Joseph Itskovitz-Eldor for assistance and cooperation in conducting this research. We would also like to thank Sean Milton and the BioMicro Center for help with slide

analysis. This work was supported by the National Science Foundation (through the MIT Biotechnology Process and Engineering Center) and the National Institutes of Health (grant no. HL60435).

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, Massachusetts, USA Daniel G Anderson, Shulamit

Levenberg & Robert Langer Authors * Daniel G Anderson View author publications You can also search for this author inPubMed Google Scholar * Shulamit Levenberg View author publications

You can also search for this author inPubMed Google Scholar * Robert Langer View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR

Correspondence to Robert Langer. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG. 1 C2C12 cells

grown on polymer arrays. (PDF 104 kb) SUPPLEMENTARY FIG. 2 Design of “hit” Polymer arrays (PDF 214 kb) SUPPLEMENTARY FIG. 3 Standard deviation of Cell coverage on Different Materials (PDF

118 kb) SUPPLEMENTARY FIG. 4 Cells per spot with RA, on day one (PDF 82 kb) SUPPLEMENTARY FIG. 5 Cells per spot with RA, on day six (PDF 81 kb) SUPPLEMENTARY TABLE 1 Percent cells

cytokeratin 7 positive on different materials over time (PDF 26 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Anderson, D., Levenberg, S. &

Langer, R. Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells. _Nat Biotechnol_ 22, 863–866 (2004). https://doi.org/10.1038/nbt981 Download

citation * Received: 20 October 2003 * Accepted: 16 April 2004 * Published: 13 June 2004 * Issue Date: 01 July 2004 * DOI: https://doi.org/10.1038/nbt981 SHARE THIS ARTICLE Anyone you share

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