ABSTRACT Polycomb group (PcG) complexes are multiprotein assemblages that bind to chromatin and establish chromatin states leading to epigenetic silencing1,2. PcG proteins regulate homeotic

genes in flies and vertebrates, but little is known about other PcG targets and the role of the PcG in development, differentiation and disease. Here, we determined the distribution of the

PcG proteins PC, E(Z) and PSC and of trimethylation of histone H3 Lys27 (me3K27) in the _D. melanogaster_ genome. At more than 200 PcG target genes, binding sites for the three PcG proteins

colocalize to presumptive Polycomb response elements (PREs). In contrast, H3 me3K27 forms broad domains including the entire transcription unit and regulatory regions. PcG targets are highly

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support SIMILAR CONTENT BEING VIEWED BY OTHERS THE ROLES OF POLYCOMB REPRESSIVE COMPLEXES IN MAMMALIAN DEVELOPMENT AND CANCER Article 15 March 2021 ADAPTATION OF GENE LOCI TO

HETEROCHROMATIN IN THE COURSE OF _DROSOPHILA_ EVOLUTION IS ASSOCIATED WITH INSULATOR PROTEINS Article Open access 17 July 2020 TRANSIENT LOSS OF POLYCOMB COMPONENTS INDUCES AN EPIGENETIC

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Exp. Morphol._ 27, 353–356 (1972). CAS  PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS We are grateful to T. Jenuwein for the anti-H3 me3K27 antibody; to D. McCabe for polytene

chromosome preparations; to A. Brooks, Q. Wang and V. Patel of the Bionomics Research and Technology Center of the Rutgers Environmental and Occupational Health Sciences Institute for

hybridization and scanning of the microarrays. Particular thanks to M. Eisen for leading the Berkeley Drosophila Transcription Network Project's development of ChIP/chip data analysis

methods and for encouraging this work. Work conducted by the BDTNP is funded by a grant from the US National Institute of General Medical Sciences and the US National Human Genome Research

Institute (GM704403) at Lawrence Berkeley National Laboratory under Department of Energy contract DE-AC02-05CH11231. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Molecular

Biology and Biochemistry, Rutgers University, 604 Allison Road, Piscataway, 08854, New Jersey, USA Yuri B Schwartz, Tatyana G Kahn & Vincenzo Pirrotta * Genomics Division, Berkeley

Drosophila Transcription Network Project, Lawrence Berkeley National Laboratory, Berkeley, 94720, California, USA David A Nix, Xiao-Yong Li & Mark Biggin * Department of Statistics,

University of California, Berkeley, Berkeley, 94720, California, USA Richard Bourgon * Affymetrix, 6550 Vallejo Street, Emeryville, 94608, California, USA David A Nix Authors * Yuri B

Schwartz View author publications You can also search for this author inPubMed Google Scholar * Tatyana G Kahn View author publications You can also search for this author inPubMed Google

Scholar * David A Nix View author publications You can also search for this author inPubMed Google Scholar * Xiao-Yong Li View author publications You can also search for this author

inPubMed Google Scholar * Richard Bourgon View author publications You can also search for this author inPubMed Google Scholar * Mark Biggin View author publications You can also search for

this author inPubMed Google Scholar * Vincenzo Pirrotta View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Vincenzo

Pirrotta. ETHICS DECLARATIONS COMPETING INTERESTS D.A.N. is employed by Affymetrix. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG. 1 Distribution of PcG proteins and H3 me3K27 mark along the X

chromosome. (PDF 81 kb) SUPPLEMENTARY FIG. 2 Distribution of PcG proteins and H3 me3K27 mark along chromosome 4. (PDF 84 kb) SUPPLEMENTARY FIG. 3 Distribution of PcG proteins and H3 me3K27

mark along 2L chromosome. (PDF 83 kb) SUPPLEMENTARY FIG. 4 Distribution of PcG proteins and H3 me3K27 mark along 2R chromosome. (PDF 81 kb) SUPPLEMENTARY FIG. 5 Distribution of PcG proteins

and H3 me3K27 mark along 3L chromosome. (PDF 82 kb) SUPPLEMENTARY FIG. 6 Co-localization between the binding of PSC and other PcG proteins. (PDF 44 kb) SUPPLEMENTARY FIG. 7 Co-localization

between the binding of E(Z) and other PcG proteins. (PDF 42 kb) SUPPLEMENTARY FIG. 8 Comparison of microarray data to the Ringrose _et al_. genome-wide prediction of PREs. (PDF 21 kb)

SUPPLEMENTARY FIG. 9 PcG proteins bind to _ph_ and _Psc-Su(z)2_ polycomb group genes. (PDF 129 kb) SUPPLEMENTARY FIG. 10 Valication of microarray hybridization results by real-time PCR. (PDF

207 kb) SUPPLEMENTARY TABLE 1 List of PcG sites. (PDF 196 kb) SUPPLEMENTARY TABLE 2 General features of PcG sites. (PDF 53 kb) SUPPLEMENTARY TABLE 3 List of PCR primers. (PDF 69 kb)

SUPPLEMENTARY METHODS (PDF 62 KB) SUPPLEMENTARY NOTE (PDF 12 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Schwartz, Y., Kahn, T., Nix, D. _et al._

Genome-wide analysis of Polycomb targets in _Drosophila melanogaster_. _Nat Genet_ 38, 700–705 (2006). https://doi.org/10.1038/ng1817 Download citation * Received: 06 February 2006 *

Accepted: 01 May 2006 * Published: 28 May 2006 * Issue Date: 01 June 2006 * DOI: https://doi.org/10.1038/ng1817 SHARE THIS ARTICLE Anyone you share the following link with will be able to

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