ABSTRACT Determining the structure of biological macromolecules by X-ray crystallography involves a series of steps: selection of the target molecule; cloning, expression, purification and

crystallization; collection of diffraction data and determination of atomic positions. However, even when pure soluble protein is available, producing high-quality crystals remains a major

bottleneck in structure determination. Here we present a guide for the non-expert to screen for appropriate crystallization conditions and optimize diffraction-quality crystal growth. Access

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SIMILAR CONTENT BEING VIEWED BY OTHERS ACCURATE COMPUTATIONAL DESIGN OF THREE-DIMENSIONAL PROTEIN CRYSTALS Article 16 October 2023 COMPREHENSIVE MICROCRYSTAL ELECTRON DIFFRACTION SAMPLE

PREPARATION FOR CRYO-EM Article 20 December 2024 MICROED FOR THE STUDY OF PROTEIN–LIGAND INTERACTIONS AND THE POTENTIAL FOR DRUG DISCOVERY Article 27 October 2021 REFERENCES * McPherson, A.

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Research Council UK (EP/D501113/1) and the European Commission OptiCryst project LSHG-CT-2006-037793. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Naomi E. Chayen is in the Department of

Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, London SW7 2AZ,

UK., Naomi E Chayen * Emmanuel Saridakis is at the Laboratory of Structural and Supramolecular Chemistry, Institute of Physical Chemistry, National Centre for Scientific Research

“Demokritos”, Ag. Paraskevi, 15310 Athens, Greece., Emmanuel Saridakis Authors * Naomi E Chayen View author publications You can also search for this author inPubMed Google Scholar *

Emmanuel Saridakis View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Naomi E Chayen. SUPPLEMENTARY INFORMATION

SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–2, Supplementary Note 1, Supplementary Protocols 1–2 (PDF 514 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE

CITE THIS ARTICLE Chayen, N., Saridakis, E. Protein crystallization: from purified protein to diffraction-quality crystal. _Nat Methods_ 5, 147–153 (2008).

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