ABSTRACT Cell division must be coordinated with chromosome replication and segregation to ensure the faithful transmission of genetic information during proliferation. In most bacteria,

assembly of the division apparatus, the divisome, starts with the polymerization of a tubulin homologue, FtsZ, into a ring-like structure at mid-cell, the Z-ring1. It typically occurs at

half of the cell cycle when most of the replication and segregation cycle of the unique chromosome they generally harbour is achieved2. The chromosome itself participates in the regulation

of cell division, at least in part because it serves as a scaffold to position FtsZ polymerization antagonists3. However, about 10% of bacteria have more than one chromosome4, which raises

which helps accurately position the _V. cholerae_ divisome at mid-cell and postpones its assembly to the very end of the cell cycle. Access through your institution Buy or subscribe This is

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CCRZ IS A PNEUMOCOCCAL SPATIOTEMPORAL CELL CYCLE REGULATOR THAT INTERACTS WITH FTSZ AND CONTROLS DNA REPLICATION BY MODULATING THE ACTIVITY OF DNAA Article Open access 09 August 2021

DYNAMIC TRANSITIONS OF INITIATOR BINDING COORDINATE THE REPLICATION OF THE TWO CHROMOSOMES IN _VIBRIO CHOLERAE_ Article Open access 08 January 2025 MATP LOCAL ENRICHMENT DELAYS SEGREGATION

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Google Scholar  Download references ACKNOWLEDGEMENTS The authors thank M.-E. Val and D. Mazel for the gift of strain MCH1 and N. Dubarry and the other team members for insightful comments.

The authors acknowledge financial support from the European Research Council under the European Community's Seventh Framework Programme (FP7/2007-2013 grant agreement no. 281590), the

Fondation Bettencourt Schueller (2012 Coup d'Elan award) and the ‘Lidex-Biologie Intégrative des Génomes’ project of the Paris-Saclay IDEX (ANR-11-IDEX-0003-02). The light microscopy

facility of Imagerie-Gif is a member of the Infrastructures en Biologie Santé et Agronomie (IBiSA), and is supported by the French national Research Agency under Investments for the Future

programmes ‘France-BioImaging’ and the Labex ‘Saclay Plant Science’ (ANR-10-INSB-04-01 and ANR-11-IDEX-0003-02, respectively). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institute for

Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris Sud, 1 avenue de la Terrasse, 91198 Gif sur Yvette, France Elisa Galli, Mickaël Poidevin, Romain

Le Bars, Jean-Michel Desfontaines, Leila Muresan, Evelyne Paly, Yoshiharu Yamaichi & François-Xavier Barre Authors * Elisa Galli View author publications You can also search for this

author inPubMed Google Scholar * Mickaël Poidevin View author publications You can also search for this author inPubMed Google Scholar * Romain Le Bars View author publications You can also

search for this author inPubMed Google Scholar * Jean-Michel Desfontaines View author publications You can also search for this author inPubMed Google Scholar * Leila Muresan View author

publications You can also search for this author inPubMed Google Scholar * Evelyne Paly View author publications You can also search for this author inPubMed Google Scholar * Yoshiharu

Yamaichi View author publications You can also search for this author inPubMed Google Scholar * François-Xavier Barre View author publications You can also search for this author inPubMed 

Google Scholar CONTRIBUTIONS E.G., M.P., Y.Y. and F.X.B. conceived and designed the experiments. E.G., M.P., R.L.B. and E.P. performed the experiments. E.G., M.P., Y.Y., J.-M.D. and F.X.B.

analysed the data. E.G., J.-M.D. and L.M. contributed reagents/materials/analysis tools. E.G., M.P., Y.Y. and F.X.B. wrote the paper. CORRESPONDING AUTHOR Correspondence to François-Xavier

Barre. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Supplementary Figures 1-10,

Supplementary Tables 1-5 and Supplementary References. (PDF 7635 kb) SUPPLEMENTARY VIDEO 1 3D FtsZ-mEos2 structures throughout the _V. cholerae_ cell cycle observed using PALM. Cells grown

in M9 minimal medium supplemented with 0.2% fructose and 1 µg ml−1 thiamine. (MP4 6440 kb) SUPPLEMENTARY VIDEO 2 Time-lapse analysis of MinD-YGFP localization in _V. cholerae_ cells. One

frame was taken every 20 seconds. Cells grown in M9 minimal medium supplemented with 0.2% fructose and 1 µg ml−1 thiamine (AVI 190 kb) SUPPLEMENTARY VIDEO 3 Time-lapse analysis of MinD-YGFP

and FtsZ-RFPT localization in _V. cholerae_ cells. One frame was taken every 20 seconds. Cells grown in M9 minimal medium supplemented with 0.2% fructose and 1 µg ml−1 thiamine. (AVI 207 kb)

SUPPLEMENTARY VIDEO 4 Mini-cell formation in MCH1. One frame was taken every 3 minutes. Cells were grown in LB (AVI 115 kb) SUPPLEMENTARY VIDEO 5 Formation of an anucleate cell after a

highly asymmetric division in MCH1. Genomic DNA is labelled with a HUα-RFPT fusion. One frame was taken every 3 minutes. Cells grown in M9 minimal medium supplemented with 0.2% fructose and

1 µg ml−1 thiamine. (AVI 55 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Galli, E., Poidevin, M., Le Bars, R. _et al._ Cell division licensing in

the multi-chromosomal _Vibrio cholerae_ bacterium. _Nat Microbiol_ 1, 16094 (2016). https://doi.org/10.1038/nmicrobiol.2016.94 Download citation * Received: 21 December 2015 * Accepted: 20

May 2016 * Published: 27 June 2016 * DOI: https://doi.org/10.1038/nmicrobiol.2016.94 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

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