ABSTRACT Spontaneous discharges of individual neurons in the suprachiasmatic nucleus (SCN) of _Clock_ mutant mice were recorded for over 5 days in organotypic slice cultures and dispersed

cell cultures using a multi-electrode dish. Circadian rhythms with periods of about 27 hours were detected in 77% of slice cultures and 15% of dispersed cell cultures derived from

_Clock/Clock_ homozygotes. These findings indicate that the _Clock_ mutation lengthens the circadian period but does not abolish the circadian oscillation, and suggest an important role of

intercellular communication in the expression of circadian rhythm in the SCN. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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2 NEURONS OF THE SUPRACHIASMATIC NUCLEUS Article Open access 09 October 2023 SUPRACHIASMATIC VIP NEURONS ARE REQUIRED FOR NORMAL CIRCADIAN RHYTHMICITY AND COMPRISED OF MOLECULARLY DISTINCT

SUBPOPULATIONS Article Open access 02 September 2020 INTEGRITY OF THE CIRCADIAN CLOCK DETERMINES REGULARITY OF HIGH-FREQUENCY AND DIURNAL LFP RHYTHMS WITHIN AND BETWEEN BRAIN AREAS Article

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  Google Scholar  Download references ACKNOWLEDGEMENTS We thank J. S. Takahashi and N. Ishida for generous gifts of Clock mutant mice, and T. Yasuda for technical assistance. This work was

supported in part by a Grant-in-Aid for scientific research from the Ministry of Education, Science, Culture, Sports and Technology of Japan (nos. 11233201 and 12557005). AUTHOR INFORMATION

AUTHORS AND AFFILIATIONS * Department of Physiology, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan Wataru Nakamura, Sato Honma & Ken-ichi Honma * Department

of Oral Functional Science, Hokkaido University Graduate School of Dentistry, Sapporo, 060-8586, Japan Wataru Nakamura & Tetsuo Shirakawa Authors * Wataru Nakamura View author

publications You can also search for this author inPubMed Google Scholar * Sato Honma View author publications You can also search for this author inPubMed Google Scholar * Tetsuo Shirakawa

View author publications You can also search for this author inPubMed Google Scholar * Ken-ichi Honma View author publications You can also search for this author inPubMed Google Scholar

CORRESPONDING AUTHOR Correspondence to Sato Honma. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG.

1. Representative wheel-running activities of _Clock_ mutant and wild-type mice. Wheel-running activities were measured under DD after recording under light/dark (LD) cycle for 10 d. White

and black bars indicated times of lights-on and lights-off, respectively. Detection of a circadian rhythm and calculation of a period were done by a χ-square periodogram using a record of

every 10 d under DD. The circadian periods of these mice in the first, second and third 10-d periods mice were 24.1, 24.5 and 24.9 h for _Clock_/+ and 23.5, 23.6 and 23.5 h for +/+,

respectively. _Clock/Clock_ showed significant circadian rhythm with a period of 28.0 h in the first 10 d under DD and then became arrhythmic. (GIF 38 kb) SUPPLEMENTARY FIG. 2. A

phase-contrast photomicrograph of a cultured SCN slice on a multi-electrode dish after 14 d in culture. A pair of the SCN was recognized as densely packed neuronal areas located immediately

above the degenerated optic chiasm together with electrodes. The black squares indicated the 64 electrodes with a size of 20 µm square arranged in an 8 × 8 pattern with 100 µm separation. V,

third ventricle; OC, optic chiasm; SCN, suprachiasmatic nucleus. Scale bar, 150 µm (GIF 184 kb) SUPPLEMENTARY METHODS (PDF 27 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS

ARTICLE CITE THIS ARTICLE Nakamura, W., Honma, S., Shirakawa, T. _et al._ _Clock_ mutation lengthens the circadian period without damping rhythms in individual SCN neurons. _Nat Neurosci_ 5,

399–400 (2002). https://doi.org/10.1038/nn843 Download citation * Received: 02 January 2002 * Accepted: 25 February 2002 * Published: 15 April 2002 * Issue Date: 01 May 2002 * DOI:

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