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Access through your institution Buy or subscribe The authors reasoned that CiVDS probably requires a voltage sensor (likely to be found in voltage-gated channels), vesicle fusion machinery
and a molecular means of linking the two. The authors tested the effects of various antagonists of voltage-gated channels on CiVDS — which can be detected as a 'jump' in membrane
capacitance (_C_m) elicited by depolarization in a Ca2+-free solution — in freshly isolated mouse DRG neurons. CiVDS was strongly inhibited by an antagonist of N-type voltage-gated calcium
channels (which contain the subunit CaV2.2). Moreover, short-hairpin RNA (shRNA)-mediated knock down of CaV2.2 in DRG neurons markedly reduced CiVDS, further supporting a role for these
channels in CiVDS. To determine which functions of the CaV2.2 channel are necessary for CiVDS, the authors introduced mutations in the voltage-sensing motif S4 or in the channel-pore region
and expressed these mutant channels in DRG neurons that had been in culture for 3 days and had spontaneously reduced levels of endogenous CaV2.2 and impaired CiVDS compared with freshly
isolated neurons. Whereas overexpression of wild-type or pore-mutant CaV2.2 rescued CiVDS in these cells, overexpression of S4-mutant CaV2.2 did not, implying that the voltage-sensing
function of CaV2.2 is necessary for CiVDS. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Access Nature and 54 other
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are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Chai, Z. et al. CaV2.2
gates calcium-independent but voltage-dependent secretion in mammalian sensory neurons. _Neuron_ http://dx.doi.org/10.1016/j.neuron.2017.10.028 (2017) Download references Authors * Natasha
Bray View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bray, N. A
discrete form of secretion. _Nat Rev Neurosci_ 19, 7 (2018). https://doi.org/10.1038/nrn.2017.162 Download citation * Published: 14 December 2017 * Issue Date: January 2018 * DOI:
https://doi.org/10.1038/nrn.2017.162 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not
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