ABSTRACT Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an _in_ternal pro_tein_ (intein).

Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external pro_tein_ (extein) and an intein. Most, but not all inteins,

contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein

found in the gyrase A subunit from _Mycobacterium xenopi_ at 2.2 Å resolution. The structure contains an unusual β-fold with the catalytic splice junctions at the ends of two adjacent

in a pre-spliced state. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS AN ALTERNATIVE DOMAIN-SWAPPED STRUCTURE OF THE _PYROCOCCUS HORIKOSHII_ POLII MINI-INTEIN Article Open access 03 June 2021

CONDITIONAL PROTEIN SPLICING OF THE _MYCOBACTERIUM TUBERCULOSIS_ RECA INTEIN IN ITS NATIVE HOST Article Open access 05 September 2024 INOSITOL HEXAKISPHOSPHATE IS REQUIRED FOR INTEGRATOR

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homology between hedgehog and self-splicing proteins. _Cell_ 91, 85–97 (1997). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Biochemistry and Biophysics, Texas A&M University, College Station, Texas, 77843-2128, USA Thomas Klabunde, Sujata Sharma & James C. Sacchettini * Institute for Medical Microbiology,

University of Bern, 3010, Bern, Switzerland Amalio Telenti * Departments of Microbiology and Immunology and Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New

York, 10461, USA William R. Jacobs Jr. Authors * Thomas Klabunde View author publications You can also search for this author inPubMed Google Scholar * Sujata Sharma View author publications

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View author publications You can also search for this author inPubMed Google Scholar * James C. Sacchettini View author publications You can also search for this author inPubMed Google

Scholar CORRESPONDING AUTHOR Correspondence to James C. Sacchettini. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Klabunde, T., Sharma, S., Telenti,

A. _et al._ Crystal structure of GyrA intein from _Mycobacterium xenopi_ reveals structural basis of protein splicing. _Nat Struct Mol Biol_ 5, 31–36 (1998).

https://doi.org/10.1038/nsb0198-31 Download citation * Received: 28 September 1997 * Accepted: 07 November 1997 * Issue Date: 01 January 1998 * DOI: https://doi.org/10.1038/nsb0198-31 SHARE

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