ABSTRACT CtBPs form NADH-sensitive chromatin-modifying complexes, which link cellular metabolism to gene transcription. They also function in the cytoplasm to regulate Golgi fissioning;

their inhibition can consequently cause a Golgi-dependent checkpoint in G2. We have recently identified a novel role of CtBPs in the maintenance of mitotic fidelity; inhibition of CtBP

synthesis resulting in reduced association of aurora B with mitotic chromatin and aberrant segregation of chromosomes. Here, we demonstrate that it is the interaction of CtBPs with

transcriptional regulators and/or chromatin-modifying enzymes in the cell nucleus, rather than their role in Golgi fission, which is critical for the maintenance of mitotic fidelity. Access

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references ACKNOWLEDGEMENTS We are grateful to Matthew Darley for the generation of plasmid reagents, Patrick Duriez for the preparation of GST fusion proteins for microinjection, and the

Southampton School of Medicine Bioimaging Unit for assistance with live cell imaging. Funding for the work was from the UK Breast Cancer Campaign. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS

* Southampton Cancer Research UK Centre, University of Southampton School of Medicine, Southampton, Hampshire, UK C N Birts & J P Blaydes * Medicines Toxicology Evaluation Section,

Office of Prescription Medicines, Therapeutic Goods Administration, Woden, Australian Capital Territory, Australia L M Bergman Authors * C N Birts View author publications You can also

search for this author inPubMed Google Scholar * L M Bergman View author publications You can also search for this author inPubMed Google Scholar * J P Blaydes View author publications You

can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to J P Blaydes. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no conflict of

interest ADDITIONAL INFORMATION Supplementary Information accompanies the paper on the Oncogene website SUPPLEMENTARY INFORMATION SUPPLEMENTAL VIDEO 1 (MOV 347 KB) SUPPLEMENTAL VIDEO 2 (MOV

367 KB) SUPPLEMENTAL VIDEO 3 (MOV 338 KB) SUPPLEMENTAL VIDEO LEGENDS (DOC 33 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Birts, C., Bergman, L.

& Blaydes, J. CtBPs promote mitotic fidelity through their activities in the cell nucleus. _Oncogene_ 30, 1272–1280 (2011). https://doi.org/10.1038/onc.2010.507 Download citation *

Received: 21 January 2010 * Revised: 20 July 2010 * Accepted: 30 September 2010 * Published: 08 November 2010 * Issue Date: 17 March 2011 * DOI: https://doi.org/10.1038/onc.2010.507 SHARE

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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * aurora B * CtBP proteins * BARS * mitosis * transcriptional repressor.