ABSTRACT BACKGROUND: Prostate cancer (PCa) harbors a myriad of genomic and epigenetic defects. Cytosine methylation of CpG-rich promoter DNA is an important mechanism of epigenetic gene

inactivation in PCa. There is considerable amount of data to suggest that DNA methylation-based biomarkers may be useful for the early detection and diagnosis of PCa. In addition, candidate

gene-based studies have shown an association between specific gene methylation and alterations and clinicopathologic indicators of poor prognosis in PCa. METHODS: To more comprehensively

identify DNA methylation alterations in PCa initiation and progression, we examined the methylation status of 485 577 CpG sites from regions with a broad spectrum of CpG densities,

<0.05; _β_-value ⩾0.2; fold change >1.5). Of the 33 genes, hierarchical clustering analysis demonstrated _BNC_1, _FZD_1, _RPL_39L, _SYN_2, _LMX_1B, _CXXC_5, _ZNF_783 and _CYB_5R2 as

top candidate novel genes that are frequently methylated and whose methylation was associated with inactivation of gene expression in PCa cell lines. Pathway analysis of the genes with

altered methylation patterns identified the involvement of a cancer-related network of genes whose activity may be regulated by _TP_53, _MYC_, _TNF_, _IL_1 and 6, _IFN_-γ and FOS in prostate

pathogenesis. CONCLUSION: Our genome-wide methylation profile shows epigenetic dysregulation of important regulatory signals in prostate carcinogenesis. Access through your institution Buy

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AN INTEGRATIVE MULTI-OMICS ANALYSIS TO IDENTIFY CANDIDATE DNA METHYLATION BIOMARKERS RELATED TO PROSTATE CANCER RISK Article Open access 06 August 2020 THE DNA METHYLATION LANDSCAPE OF

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Program Idea Award; PC101996 to Bernard Kwabi-Addo. This work is also supported by the use of facilities at Howard University Department of Biochemistry and Molecular Biology, and Children’s

National Medical Center. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Children’s National Medical Center, Center for Genetic Medicine Research, Washington, DC, USA J M Devaney & S

Funda * Cancer Center, Howard University, Washington, DC, USA S Wang, J Long, D J Taghipour, P Furbert-Harris & B Kwabi-Addo * Department of Biochemistry and Molecular Biology, Howard

University, Washington, DC, USA S Wang, R Tbaishat & B Kwabi-Addo * Department of Microbiology, Howard University, Washington, DC, USA P Furbert-Harris * Department of Pathology and

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permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Devaney, J., Wang, S., Funda, S. _et al._ Identification of novel DNA-methylated genes that correlate with human prostate cancer and

high-grade prostatic intraepithelial neoplasia. _Prostate Cancer Prostatic Dis_ 16, 292–300 (2013). https://doi.org/10.1038/pcan.2013.21 Download citation * Received: 07 March 2013 *

Revised: 24 June 2013 * Accepted: 27 June 2013 * Published: 30 July 2013 * Issue Date: December 2013 * DOI: https://doi.org/10.1038/pcan.2013.21 SHARE THIS ARTICLE Anyone you share the

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Nature SharedIt content-sharing initiative KEYWORDS * genome-wide DNA methylation analysis * pyrosequencing * gene expression