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ABSTRACT Human milk leukocytes contain large quantities of IgA and other proteins. During prolonged in vitro incubation, the IgA is released. To further examine the potential role of this
process in delivering these proteins to the recepient infant, the effect of phagocytosis upon the release of IgA from colostral leukocytes was investigated. Washed leukocytes were incubated
with live Escherichia coli or heat-killed Candida albicans in the presence or absence of serum opsonins. The degree of phagocytosis was determined microscopically. Controls were incubated
without particles. After 15, 30, and 60 minutes, cells and supernatant fluids were recovered and assayed for total IgA and SIgA by a quantitative immunofluorescence assay. The degree of IgA
release was enhanced (approximately 40%) with cells exposed to opsonized organisms, as compared to preparations containing no particles. The degree of decrease in the IgA level in the cell
lysates was paralleled by an increase in IgA in the supernatant fluids from the cell preparations. In contrast, little release occurred (approximately 4%) with unopsonized organisms. The
enhanced release was evident within 15 minutes. Cells incubated with opsonized organisms at 4°C did not release IgA until warmed. The enhanced release of IgA by colostral leukocytes during
phagocytosis may be part of a mechanism to deliver IgA antibodies to the site of microbial colonization or infection in the infant. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *
The Departments of Microbiology and Pediatrics, University of Texas Medical Branch, Galveston Elizabeth A Weaver, Armond S Goldman, Randall M Goldblum & Charles P Davis Authors *
Elizabeth A Weaver View author publications You can also search for this author inPubMed Google Scholar * Armond S Goldman View author publications You can also search for this author
inPubMed Google Scholar * Randall M Goldblum View author publications You can also search for this author inPubMed Google Scholar * Charles P Davis View author publications You can also
search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Weaver, E., Goldman, A., Goldblum, R. _et al._ 974
COLOSTRAL CELLS: IgA RELKASE STIMULATED DURING PHAGOCYTOSIS. _Pediatr Res_ 15 (Suppl 4), 605 (1981). https://doi.org/10.1203/00006450-198104001-00999 Download citation * Issue Date: 01 April
1981 * DOI: https://doi.org/10.1203/00006450-198104001-00999 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a
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