1002 quantitative protection studies in a suckling pat model of group b streptccoccal sepsis

1002 quantitative protection studies in a suckling pat model of group b streptccoccal sepsis

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ABSTRACT Group B streptococci (GBS) are a major cause of sepsis and meningitis in the newborn. Neonatal susceptibility to GBS infection appears to be associated with a deficiency of opsonic antibody to GBS. The present studies were designed to determine if human antibodies protective for one type III GBS strain provide uniform protection for other type III strains. IgG was affinity purified from pooled human immunoglobulin using a staphylococcal protein A immunoabsorbant. The relative titers of IgG antibodies to group B and type III (native and core) antigens were determined. To evaluate protection, suckling rats were challenged S.Q. with each of 4 different clinical isolates of GBS type III followed immediately by various concentrations of purified IgG given IP. With 5 or 10 μg of IgG significant survival occurred in animals challenged with one strain (17/20, 85%), whereas this amount of antibody afforded no protection against the other 3 strains (0/27). Administration of 20 μg of IgG provided some protection for 2 of these strains (7/23, 3/9), but uniform lethality occurred with the fourth strain even when more than 40 μg IgG was given. These studies demonstrate that four type III GBS strains require different amounts of human IgG for protection. Strain variability must be examined in future investigations of vaccine induced or passively acquired IgG for protection against GBS disease. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, Uniformed Services University of the Health Sciences, Bethesda, 20014, MD. Gerald W Fischer, Samuel R Wilson, Kenneth W Hunter & Val G Hemming Authors * Gerald W Fischer View author publications You can also search for this author inPubMed Google Scholar * Samuel R Wilson View author publications You can also search for this author inPubMed Google Scholar * Kenneth W Hunter View author publications You can also search for this author inPubMed Google Scholar * Val G Hemming View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fischer, G., Wilson, S., Hunter, K. _et al._ 1002 QUANTITATIVE PROTECTION STUDIES IN A SUCKLING PAT MODEL OF GROUP B STREPTCCOCCAL SEPSIS. _Pediatr Res_ 15 (Suppl 4), 609 (1981). https://doi.org/10.1203/00006450-198104001-01028 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01028 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT Group B streptococci (GBS) are a major cause of sepsis and meningitis in the newborn. Neonatal susceptibility to GBS infection appears to be associated with a deficiency of opsonic


antibody to GBS. The present studies were designed to determine if human antibodies protective for one type III GBS strain provide uniform protection for other type III strains. IgG was


affinity purified from pooled human immunoglobulin using a staphylococcal protein A immunoabsorbant. The relative titers of IgG antibodies to group B and type III (native and core) antigens


were determined. To evaluate protection, suckling rats were challenged S.Q. with each of 4 different clinical isolates of GBS type III followed immediately by various concentrations of


purified IgG given IP. With 5 or 10 μg of IgG significant survival occurred in animals challenged with one strain (17/20, 85%), whereas this amount of antibody afforded no protection against


the other 3 strains (0/27). Administration of 20 μg of IgG provided some protection for 2 of these strains (7/23, 3/9), but uniform lethality occurred with the fourth strain even when more


than 40 μg IgG was given. These studies demonstrate that four type III GBS strains require different amounts of human IgG for protection. Strain variability must be examined in future


investigations of vaccine induced or passively acquired IgG for protection against GBS disease. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, Uniformed


Services University of the Health Sciences, Bethesda, 20014, MD. Gerald W Fischer, Samuel R Wilson, Kenneth W Hunter & Val G Hemming Authors * Gerald W Fischer View author publications


You can also search for this author inPubMed Google Scholar * Samuel R Wilson View author publications You can also search for this author inPubMed Google Scholar * Kenneth W Hunter View


author publications You can also search for this author inPubMed Google Scholar * Val G Hemming View author publications You can also search for this author inPubMed Google Scholar RIGHTS


AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fischer, G., Wilson, S., Hunter, K. _et al._ 1002 QUANTITATIVE PROTECTION STUDIES IN A SUCKLING PAT MODEL OF


GROUP B STREPTCCOCCAL SEPSIS. _Pediatr Res_ 15 (Suppl 4), 609 (1981). https://doi.org/10.1203/00006450-198104001-01028 Download citation * Issue Date: 01 April 1981 * DOI:


https://doi.org/10.1203/00006450-198104001-01028 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is


not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative