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ABSTRACT Type 2 alveolar epithelial cells synthesize a surfactant which is important for maintaining lung stability. To isolate these cells, fetal rabbit lungs (27-29 days gestation) were
digested enzymatically, and differential adhesion to plastic substrate was used to obtain a population enriched in epithelial cells. Cells were cultured using selective medium (with D-valine
instead of L-valine) to suppress growth of contaminating fibroblasts; after 4-5 days, the cultures were confluent. Cells were examined at electron microscope level; in a typical culture
from 29 day fetuses, approx. 10-40% were identified as “definite” type 2 (cuboidal or elliptical, surface microvilli, cytoplasmic lamellar bodies), and 50-60% were identified as “probable”
type 2 (same criteria as for “definite”, except no lamellar bodies). The cells incorporated (14C)-choline into disaturated phosphatidylcholine (dspc), a major component of lung surfactant.
Specific activities of the acyl transferase system were compared using palmitoyl-CoA and oleyl-CoA as donor substrates, and 1-sat-2-lyso-pc as receptor substrate. At 27 days gestation, the
ratio (palmitoyl-CoA/oleyl-CoA) was 0.39, while at 29 days the ratio was 3.5. These data suggest that changes in acyl transferase donor specificity are important in regulating synthesis of
dspc. We conclude that this culture system is a useful model for studying metabolism of lung surfactant. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Albert Einstein College of
Medicine and Department of Pathology, Pediatric Pulmonary Div., St. Lukes-Roosevelt Hospital Medical Center, New York, New York Gary L Sanford, Suck K Lee, Stephen F Ryan, Michael F
Frosolono, Robert S Bienkowski & Emile M Scarpelli Authors * Gary L Sanford View author publications You can also search for this author inPubMed Google Scholar * Suck K Lee View author
publications You can also search for this author inPubMed Google Scholar * Stephen F Ryan View author publications You can also search for this author inPubMed Google Scholar * Michael F
Frosolono View author publications You can also search for this author inPubMed Google Scholar * Robert S Bienkowski View author publications You can also search for this author inPubMed
Google Scholar * Emile M Scarpelli View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE
CITE THIS ARTICLE Sanford, G., Lee, S., Ryan, S. _et al._ 1718 CULTURE OF TYPE 2 CELLS FROM FETAL RABBIT LUNGS. _Pediatr Res_ 15 (Suppl 4), 730 (1981).
https://doi.org/10.1203/00006450-198104001-01737 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01737 SHARE THIS ARTICLE Anyone you share the
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