1718 culture of type 2 cells from fetal rabbit lungs

1718 culture of type 2 cells from fetal rabbit lungs

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ABSTRACT Type 2 alveolar epithelial cells synthesize a surfactant which is important for maintaining lung stability. To isolate these cells, fetal rabbit lungs (27-29 days gestation) were digested enzymatically, and differential adhesion to plastic substrate was used to obtain a population enriched in epithelial cells. Cells were cultured using selective medium (with D-valine instead of L-valine) to suppress growth of contaminating fibroblasts; after 4-5 days, the cultures were confluent. Cells were examined at electron microscope level; in a typical culture from 29 day fetuses, approx. 10-40% were identified as “definite” type 2 (cuboidal or elliptical, surface microvilli, cytoplasmic lamellar bodies), and 50-60% were identified as “probable” type 2 (same criteria as for “definite”, except no lamellar bodies). The cells incorporated (14C)-choline into disaturated phosphatidylcholine (dspc), a major component of lung surfactant. Specific activities of the acyl transferase system were compared using palmitoyl-CoA and oleyl-CoA as donor substrates, and 1-sat-2-lyso-pc as receptor substrate. At 27 days gestation, the ratio (palmitoyl-CoA/oleyl-CoA) was 0.39, while at 29 days the ratio was 3.5. These data suggest that changes in acyl transferase donor specificity are important in regulating synthesis of dspc. We conclude that this culture system is a useful model for studying metabolism of lung surfactant. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Albert Einstein College of Medicine and Department of Pathology, Pediatric Pulmonary Div., St. Lukes-Roosevelt Hospital Medical Center, New York, New York Gary L Sanford, Suck K Lee, Stephen F Ryan, Michael F Frosolono, Robert S Bienkowski & Emile M Scarpelli Authors * Gary L Sanford View author publications You can also search for this author inPubMed Google Scholar * Suck K Lee View author publications You can also search for this author inPubMed Google Scholar * Stephen F Ryan View author publications You can also search for this author inPubMed Google Scholar * Michael F Frosolono View author publications You can also search for this author inPubMed Google Scholar * Robert S Bienkowski View author publications You can also search for this author inPubMed Google Scholar * Emile M Scarpelli View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sanford, G., Lee, S., Ryan, S. _et al._ 1718 CULTURE OF TYPE 2 CELLS FROM FETAL RABBIT LUNGS. _Pediatr Res_ 15 (Suppl 4), 730 (1981). https://doi.org/10.1203/00006450-198104001-01737 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01737 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT Type 2 alveolar epithelial cells synthesize a surfactant which is important for maintaining lung stability. To isolate these cells, fetal rabbit lungs (27-29 days gestation) were


digested enzymatically, and differential adhesion to plastic substrate was used to obtain a population enriched in epithelial cells. Cells were cultured using selective medium (with D-valine


instead of L-valine) to suppress growth of contaminating fibroblasts; after 4-5 days, the cultures were confluent. Cells were examined at electron microscope level; in a typical culture


from 29 day fetuses, approx. 10-40% were identified as “definite” type 2 (cuboidal or elliptical, surface microvilli, cytoplasmic lamellar bodies), and 50-60% were identified as “probable”


type 2 (same criteria as for “definite”, except no lamellar bodies). The cells incorporated (14C)-choline into disaturated phosphatidylcholine (dspc), a major component of lung surfactant.


Specific activities of the acyl transferase system were compared using palmitoyl-CoA and oleyl-CoA as donor substrates, and 1-sat-2-lyso-pc as receptor substrate. At 27 days gestation, the


ratio (palmitoyl-CoA/oleyl-CoA) was 0.39, while at 29 days the ratio was 3.5. These data suggest that changes in acyl transferase donor specificity are important in regulating synthesis of


dspc. We conclude that this culture system is a useful model for studying metabolism of lung surfactant. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Albert Einstein College of


Medicine and Department of Pathology, Pediatric Pulmonary Div., St. Lukes-Roosevelt Hospital Medical Center, New York, New York Gary L Sanford, Suck K Lee, Stephen F Ryan, Michael F


Frosolono, Robert S Bienkowski & Emile M Scarpelli Authors * Gary L Sanford View author publications You can also search for this author inPubMed Google Scholar * Suck K Lee View author


publications You can also search for this author inPubMed Google Scholar * Stephen F Ryan View author publications You can also search for this author inPubMed Google Scholar * Michael F


Frosolono View author publications You can also search for this author inPubMed Google Scholar * Robert S Bienkowski View author publications You can also search for this author inPubMed 


Google Scholar * Emile M Scarpelli View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE


CITE THIS ARTICLE Sanford, G., Lee, S., Ryan, S. _et al._ 1718 CULTURE OF TYPE 2 CELLS FROM FETAL RABBIT LUNGS. _Pediatr Res_ 15 (Suppl 4), 730 (1981).


https://doi.org/10.1203/00006450-198104001-01737 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01737 SHARE THIS ARTICLE Anyone you share the


following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer


Nature SharedIt content-sharing initiative