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The recent advent of genome-scale imaging has enabled single-cell omics analysis in a spatially resolved manner in intact cells and tissues. These advances allow gene expression profiling of
individual cells, and hence in situ identification and spatial mapping of cell types, in complex tissues. The high spatial resolution of these approaches further allows determination of the
spatial organizations of the genome and transcriptome inside cells, both of which are key regulatory mechanisms for gene expression. Access through your institution Buy or subscribe This is
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I am grateful to J. Moffitt for suggestions and comments on the paper, and to J. Moffitt, B. Bintu and J. Fan for help with figures. This work is supported in part by the National
Institutes of Health. X.Z. is a Howard Hughes Medical Institute investigator. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Howard Hughes Medical Institute, Cambridge, MA, USA Xiaowei Zhuang
* Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA Xiaowei Zhuang * Department of Physics, Harvard University, Cambridge, MA, USA Xiaowei Zhuang Authors
* Xiaowei Zhuang View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Xiaowei Zhuang. ETHICS DECLARATIONS COMPETING
INTERESTS X.Z. is a cofounder and consultant of Vizgen and an inventor on patents applied for by Harvard University related to MERFISH. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT
THIS ARTICLE CITE THIS ARTICLE Zhuang, X. Spatially resolved single-cell genomics and transcriptomics by imaging. _Nat Methods_ 18, 18–22 (2021). https://doi.org/10.1038/s41592-020-01037-8
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