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ABSTRACT RECENTLY, Carroll1 advocated the use of the product of retention time (_t__R_) and peak height (_h_) for the quantitative estimation of relative peak areas. This method2, based on
the assumption of a proportionality of peak width (_w_) and retention time (_t__R_) or volume (_V__R_), gives satisfactory results with respect to the reproducibility. In fact, we have found
that the reproducibility (under equal operating conditions) of this method is better than that of any other ‘manual’ estimation of peak areas. However, the method is not commonly used in
our Laboratory, as the results obtained with it are erratic. This is due to the fact that in most cases the proportionality between _w_ and _V__R_ is only approximate. Differences between
the relative areas, as found by the triangulation method and from the product of _h_ and _V__R_, can amount to several per cent relative. In principle the non-linearity of _w_ and _V__R_ can
be taken into account when calibrating the detector. However, changing the column or altering the carrier gas velocity will change the calibration factors. SIMILAR CONTENT BEING VIEWED BY
OTHERS REAL-TIME GAS MASS SPECTROSCOPY BY MULTIVARIATE ANALYSIS Article Open access 13 April 2023 COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY Article 16 November 2023 COMPREHENSIVE
TWO-DIMENSIONAL GAS CHROMATOGRAPHY–MASS SPECTROMETRY Article 06 February 2025 ARTICLE PDF REFERENCES * Carroll, K. K., _Nature_, 191, 377 (1961). Article ADS CAS Google Scholar *
Phillips, C., _Gas Chromatography_, 63 (Butterworth, London, 1956). Google Scholar * Keulemans, A. I. M., _Gas Chromatography_, 127 (Reinhold Pub., New York, 1959). Google Scholar * van
Deemter, J. J., Zuiderweg, F. J., and Klinkenberg, A., _Chem. Eng. Sci._, 5, 271 (1956). Article CAS Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *
Central Laboratory, Staatsmijnen in Limburg, Geleen, The Netherlands A. DIJKSTRA Authors * A. DIJKSTRA View author publications You can also search for this author inPubMed Google Scholar
RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE DIJKSTRA, A. Quantitative Estimation of Peak Areas in Gas-Liquid Chromatography. _Nature_ 192, 965
(1961). https://doi.org/10.1038/192965a0 Download citation * Issue Date: 09 December 1961 * DOI: https://doi.org/10.1038/192965a0 SHARE THIS ARTICLE Anyone you share the following link with
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