Access through your institution Buy or subscribe Recent findings illustrate that diseases are often accompanied by changes in the numbers or function of `fine' lymphocyte subsets, even

if changes in the bulk lymphocyte populations are not evident. In some cases, such changes may provide powerful prognostic and diagnostic information. For example, Giorgi _et al_.1 showed

that the number of activated CD8 T cells (CD38+/HLA-DR+), but not the number of total CD8 T cells, was a better predictor of progression to AIDS than the commonly-used CD4 T-cell count. And,

by accurately dividing T cells into naive and memory, we demonstrated that progression of HIV disease was accompanied by the loss of both CD4 and CD8 naive T cells2,3—a finding that could

(absolute number or percentage) of fine subsets of lymphocytes are also correlated with disease. Identifying such changes has been difficult because traditional three-color flow cytometric

analyses can only resolve major lineages (B, T, natural killer, monocyte or neutrophil), or, at best, resolve a few subsets of any single lineage. This is a preview of subscription content,

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OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Giorgi, J.V. et al. Elevated levels of CD38+ CD8+ T cells in HIV infection

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Scholar  Download references ACKNOWLEDGEMENTS This work was supported by grants from the NIH (CA-42509-14, CA-81543-02), and from the University of California University-wide AIDS Research

Program (F97-ST-044). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland, USA Stephen C. De Rosa &

 Mario Roederer * Department of Genetics, Stanford University School of Medicine, Stanford, California, USA Leonard A. Herzenberg & Leonore A. Herzenberg Authors * Stephen C. De Rosa

View author publications You can also search for this author inPubMed Google Scholar * Leonard A. Herzenberg View author publications You can also search for this author inPubMed Google

Scholar * Leonore A. Herzenberg View author publications You can also search for this author inPubMed Google Scholar * Mario Roederer View author publications You can also search for this

author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Mario Roederer. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE De Rosa, S.,

Herzenberg, L., Herzenberg, L. _et al._ 11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity. _Nat Med_ 7,

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