ABSTRACT Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA

vaccine encoding _env, rev_ and _gag/pol_ in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a

heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated

infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark

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INDUCED LONG-TERM IMMUNITY AGAINST NIPAH VIRUS INFECTION Article Open access 04 November 2023 HETEROLOGOUS VACCINATION REGIMENS WITH SELF-AMPLIFYING RNA AND ADENOVIRAL COVID VACCINES INDUCE

ROBUST IMMUNE RESPONSES IN MICE Article Open access 17 May 2021 INFECTIOUS RNA VACCINE PROTECTS MICE AGAINST CHIKUNGUNYA VIRUS INFECTION Article Open access 03 December 2020 REFERENCES *

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authors contributed equally to this manuscript. AUTHORS AND AFFILIATIONS * Department of Pathology and Laboratory Medicine, University of Pennsylvania, SOS Stellar-Chance Building, 422

Curie Boulevard, Philadelphia, Pennsylvania, 19104, USA Jean D. Boyer, Bin Wang, Michael Agadjanyan, Lori Gilbert, Michael Chattergoon & David B. Weiner * Department of Medical

Microbiology and Immunology, University of South Florida, MDC10 12901 Bruce B. Downs Boulevard, Tampa, Florida, 33612, USA Kenneth E. Ugen * Department of Pediatrics, University of

Pennsylvania, 505 Stellar-Chance Building, 422 Curie Boulevard, Philadelphia, Pennsylvania, 19104, USA Mark L. Bagarazzi * White Sands Research Center, Coulston Foundation, 1300 Lavelle

Road, Alamogordo, New Mexico, 88310, USA Patrice Frost & Ali Javadian * Hospital of the University of Pennsylvania, 912 Stellar-Chance Building, 422 Curie Boulevard, Philadelphia,

Pennsylvania, 19104, USA Williams V. Williams * Department of Pathology, Harvard University, LMRC-5, 221 Longwood Avenue, Boston, Massachusetts, 02115, USA Yosef Refaeli * Apollon Inc., 1

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PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Boyer, J., Ugen, K., Wang, B. _et al._ Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA

vaccination. _Nat Med_ 3, 526–532 (1997). https://doi.org/10.1038/nm0597-526 Download citation * Received: 21 November 1996 * Accepted: 04 March 1997 * Issue Date: 01 May 1997 * DOI:

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