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ABSTRACT Several methods have been introduced for the assessment of chemotactic activity of mononuclear and polymorphonuclear leucocytes /PMKs/. These techniques mostly base on the original
assay of Boyden. We have recently devised a simple migration test modifying a commercially available Huclepore filter holder /EFH/ and filter membrane /pore diameter 5 μm, pore length 10 μm,
Huclepore Corporation, Pleasanton, USA/. The upper compartment of the NFH is loaded with 100 μl of isolated human PMKs suspended in Hanks' balanced salt solution. The lower compartment
contains 400 μl of the same suspension medium with or without chemoattractive agent. The loaded NFH is incubated at 37 C for 45 minutes. Then, the content of lower compartment is aspirated
and the concentration of PMNs is counted in this suspension. Preliminary experiments show the usefulness of the method which is highly sensitive to distinguishing random and directed
migrations /mean factor 3.2±0.5/. The coefficient of variation was between 12 and 18% using same suspensions. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Dept. of Anaesth. and
Intensive Care, Med. School of Pécs, Szigeti 12, 7643, Hungary L Bogar, Zs Molnar & M Tekeres Authors * L Bogar View author publications You can also search for this author inPubMed
Google Scholar * Zs Molnar View author publications You can also search for this author inPubMed Google Scholar * M Tekeres View author publications You can also search for this author
inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bogar, L., Molnar, Z. & Tekeres, M. THE NUCLEPORE FILTER HOLDER: A NEW METHOD
FOR CELL MIGRATION TEST. _Pediatr Res_ 22, 220 (1987). https://doi.org/10.1203/00006450-198708000-00042 Download citation * Issue Date: 01 August 1987 * DOI:
https://doi.org/10.1203/00006450-198708000-00042 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is
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