ABSTRACT Recent studies have suggested that lincRNA-ROR is involved in the tumorigenesis of different types of cancers. However, the role of lincRNA-ROR in retinoblastoma has not been

determined. We investigated lincRNA-ROR levels in 58 retinoblastoma and adjacent non-tumor tissues by quantitative reverse transcription PCR. Recurrence-free survival was analyzed using Cox

regression analyses. Cell migration and invasion abilities were detected by wound-healing, Transwell invasion, and bioluminescence imaging assays. Western blotting was performed to detect

epithelial–mesenchymal transition markers. Interactions between lincRNA-ROR, miR-32-5p, and Notch1 were confirmed by Luciferase, RNA pull-down, and RIP assays. Histone acetylation was

in vitro. Further, we demonstrated that lincRNA-ROR activates the Notch signaling pathway by acting as a sponge of miR-32-5p. Upregulation of lincRNA-ROR was attributed to the CBP-mediated

H3K27 acetylation at the promoter region. Our results reveal a potential competing endogenous RNA regulatory pathway, in which lincRNA-ROR modulates the epithelial–mesenchymal transition

program by competitively binding to endogenous miR-32-5p and regulating Notch signaling pathway activity in retinoblastoma cells, which may provide new insights into novel molecular

therapeutic targets for retinoblastoma. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS MIR-889-3P TARGETING BMPR2 PROMOTES THE DEVELOPMENT OF RETINOBLASTOMA VIA JNK/MAPK/ERK SIGNALING Article Open

access 27 March 2024 LINC01152 UPREGULATES MAML2 EXPRESSION TO MODULATE THE PROGRESSION OF GLIOBLASTOMA MULTIFORME VIA NOTCH SIGNALING PATHWAY Article Open access 22 January 2021 LNCRNA

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squamous cell carcinoma. Int J Oncol. 2019;54:1183–94. CAS  PubMed  PubMed Central  Google Scholar  Download references FUNDING The Project Supported by Shenzhen Science and Technology Plan

Project (Grant no. JCYJ20170307095222274), Natural Science Foundation of Guangdong Province (Grant no. 2019A1515010412), National Natural Science Foundation of China (Grant no. 81902751).

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Ophthalmology, The Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen, China Yali Gao & 

Xiaoling Luo * Department of Obstetrics and Gynecology, The Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen, China Jun Zhang Authors * Yali Gao View

author publications You can also search for this author inPubMed Google Scholar * Xiaoling Luo View author publications You can also search for this author inPubMed Google Scholar * Jun

Zhang View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Jun Zhang. ETHICS DECLARATIONS CONFLICT OF INTEREST The

authors declare that they have no conflict of interest. ETHICS STATEMENT All procedures performed in studies involving human participants and animals were in accordance with the ethical

standards of the ethic committee of the Shenzhen People’s Hospital. ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature remains neutral with regard to jurisdictional claims in published

maps and institutional affiliations. SUPPLEMENTARY INFORMATION TABLE S1 TABLE S2 RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Gao, Y., Luo, X. &

Zhang, J. LincRNA-ROR is activated by H3K27 acetylation and induces EMT in retinoblastoma by acting as a sponge of miR-32 to activate the Notch signaling pathway. _Cancer Gene Ther_ 28,

42–54 (2021). https://doi.org/10.1038/s41417-020-0181-z Download citation * Received: 23 February 2020 * Revised: 06 May 2020 * Accepted: 07 May 2020 * Published: 22 May 2020 * Issue Date:

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